Association of SNPs in exon 2 of the MHC B-F gene with immune traits in two distinct chicken populations: Chinese Beijing-You and White Leghorn

Abstract

Antibody titers raised for vaccinations against avian influenza (AI) and Newcastle disease (ND) were higher in Chinese Beijing-You (BJY) than in White Leghorn (WL) (P < 0.001), but there was no breed difference in titers for sheep red blood cells (SRBC). Genotyping by PCR-SSCP identified seven haplotypes in WL and 17 in BJY. After sequencing PCR products (35 and 85, respectively), 43 (WL) and 47 (BJY) single nucleotide polymorphisms (SNPs) were found in the 264 bp of exon 2. In WL chickens, significant associations were found with antibody responses to AI (two SNPs), ND (six SNPs), and SRBC (one SNP), while in BJY there was association with responses to ND (two SNPs) and SRBC (two SNPs), but none with AI. These results indicate that the genomic region bearing exon 2 of the major histocompatibility complex B-F gene has significant effects on antibody responses to SRBC and vaccination against AI and ND. Different SNPs affected antibody titers for each of the antigens and they differed between these very distinct breeds.

Association between Single Nucleotide Polymorphisms of the Major Histocompatibility Complex Class II Gene and Newcastle Disease Virus Titre and Body Weight in Leung Hang Khao Chickens

“…Significant associations between the SNPs and NDV titre were detected. The results support those of Du et al (2011) , Guo et al (2012) , Li et al (2012) , and Liu et al (2009) , who studied the association between various indigenous chickens. Different SNPs, however, were observed using these different populations.…”

“…We identified seven SNPs in the current study, but only SNP A126T was also detected by Li et al (2012) and Liu et al (2009) , who investigated a population of Chinese indigenous chickens. A comparison of the populations could provide information about the common ancestor of the populations and their allelic diversity.…”

“…The DNA solution was quantified spectrophotometrically and diluted to 10 μg/μL. The genotyping of MHC class II was carried out using polymerase chain reaction (PCR) with primers reported by ( Boonyanuwat et al, 2006 ; Liu et al, 2009 ).…”

Comparative evaluation of the immune responses of seven chicken ecotypes to vaccination against Newcastle disease

“…In general, commercial Bovans breed had a lower HI titer than some Ethiopian local chicken ecotypes and Fayoumi in this study. The commercial White-leg horn breed had a lower HI titer than the local chicken breed (Liu et al, 2009). The same finding was reported between commercial Fayoumi and local breeds (Deist et al, 2017).…”

“…(2-3) corresponds to 70-80 and 35-60% survival rates in infected chickens (Abdi et al, 2016). Chickens of different genetic backgrounds have differences in immune response (antibody titer) following vaccination (Newport et al, 2004;Liu et al, 2009). Geographic location, the season of birth, and the timing of vaccination can make the immune response of the host strong or weak during immunization (Hur et al, 2014).…”