Association of nymphs and adults of Ephemeroptera (Insecta) using the amplified fragment length polymorphism (AFLP) technique

Abstract

-Many species of Ephemeroptera have been described based on either nymphs or adults. Only 10% of Ephemeroptera species in South America are known in both life stages. The current research was carried out in order to molecularly associate nymphs and adults of Ephemeroptera, using the amplified fragment length polymorphism (AFLP) technique. We obtained molecular fingerprints by the AFLP of five species: Leptohyphes albipennis, Leptohyphes coconuco, Leptohyphes ecuador, Baetodes levis and Prebaetodes sitesi. The association between nymphs and adults of L. albipennis and L. coconuco by this analysis confirmed the effectiveness of the technique. The molecular fingerprints of B. levis and P. sitesi were generated with the hope that future collections of unknown adults would match the fingerprint and solve an identification problem. We describe here for the first time the adult of L. ecuador by matching an adult trapped in the field with the DNA fingerprint of nymphs obtained by means of AFLP.

Three new species of Leptohyphes Eaton (Ephemeroptera: Leptohyphidae) from Colombia

“…Six species are recorded, mainly in the Andean range of the country: L. albipennis Molineri & Zúñiga, 2006, L. ecuador Mayo, 1968(Dias et al, 2011, Leptohyphes jodiannae Allen, 1967 (Zúñiga et al, 2014), Leptohyphes maculatus Allen, 1967 (Molineri, 2010;Zúñiga et al, 2014) and L. nigripennis Molineri & Zúñiga, 2006(Molineri, 2010. All these species are known from both stages, except L. jodiannae (only known from nymphs).…”

Revisión histórica de la limnología en Colombia

“…Los primeros estudios de Ephemeroptera en Colombia se realizaron en el departamento de Antioquia, con énfasis en su taxonomía y su distribución en diferentes gradientes altitudinales (Roldán, 1980(Roldán, , 1985(Roldán, , 1988. Posteriormente, diversos autores contribuyeron al avance del conocimiento del orden en varias regiones del país mediante el registro y la descripción de nuevos géneros y especies, particularmente de las familias Leptohyphidae, Baetidae, Leptophlebiidae y Polymitarciydae (Muñoz & Ospina, 1999;Muñoz, 2000;Mosquera, et al, 2001;Domínguez, et al, 2002Domínguez, et al, , 2009Domínguez & Zúñiga, 2003Camargo & Rozo, 2003;Molineri, 2010;Molineri, et al, 2002Molineri, et al, , 2011Gutiérrez & Reinoso, 2010;Salinas, et al, 2011;Días, et al, 2011;. En la región natural andina se encuentra la mayor distribución y diversidad, así como el mayor número de registros específicos (Roldán, 1988;Reinoso, 1999;Zúñiga, et al, 2004. En cuanto al orden Odonata, el apoyo de Minter Westfall del Departamento de Zoología de la Universidad de Florida en Gainsville.…”