Association of MMP9-1562C/T and MMP13-77A/G Polymorphisms with Non-Small Cell Lung Cancer in Southern Chinese Population

Abstract

Background: Matrix metalloproteinases (MMPs) are capable of degrading and modifying most components of the extracellular matrix (ECM) and the basal membrane (BM), and play crucial roles in cancer invasion and metastasis. MMP gene expressions were regulated primarily at the transcriptional level, which was associated with tumor spread and patient prognosis. Polymorphisms in MMPs have been reported to be associated with non-small cell lung cancer (NSCLC). The objective of this study aim to evaluate the serum levels and polymorphisms of MMP-9 and MMP-13 in non-small cell lung cancer patients compared to normal subjects and their correlation to non-small cell lung cancer histopathology findings in Southern Chinese people. Methods: This case–control study included 245 patients with NSCLC and 258 healthy controls. Genomic DNA was extracted by using DNA extraction kit, genotyping was confirmed by polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) and direct DNA sequencing, and serum levels of MMP-9 and MMP-13 were measured by using a specific ELISA, Human Matrix Metalloproteinase Enzyme Immunoassay Kits. Statistical analysis was carried out using the SPSS 23.0 software package. Results: The subjects carrying the TT genotype had a decreased risk of lung cancer in MMP9-1562C/T comparing with the CC genotype (p = 0.00, OR = 0.45, 95% CI = 0.29–0.68), and the MMP13-77 AA genotype was associated with a decreased risk of NSCLC by comparing with the GG genotype (p = 0.03, OR = 0.56, 95% CI = 0.33–0.94). Moreover, the C allele of MMP9-1562C/T could increase serum level of NSCLC in compared with the A allele (OR = 1.19, 95% CI = 0.75–1.89). Similarly, the AA genotype of MMP13 might be a marker of decreased serum level of lung cancer (OR = 0.76, 95% CI = 0.51–1.14). Conclusions: The results of these analyses underline the support of the notion that the CC genotype of MMP9-1562C/T and GG genotypes of MMP13-77G/A were associated with the increased risk NSCLC, and the serum levels of MMP9 and MMP13 were consistent with the results of the SNP analysis. MMP13 and MMP9 might be function as a key oncogene in NSCLC with a Southern Chinese population. Combined detection of SNP and enzyme activity between MMP9 and MMP13 are expected to be a potential diagnostic method of non-small cell lung cancer.

Asperosaponin VI inhibits LPS-induced inflammatory response by activating PPAR-γ pathway in primary microglia

“…In this study, we found ASA VI promoted the transcriptional expression of PPAR-γ gene in LPS-treated microglia. Simultaneously, ASA VI promotes nuclear transfer of PPAR-γ in LPS-induced microglia ( Gao et al, 2017 , Gao et al, 2017 , Gao et al, 2017 , Li et al, 2019 ). These data indicated that ASA VI activated the PPAR-γ signaling pathway in LPS-induced microglia.…”

Method for accurate diagnose of lupus erythematosus skin lesions based on microbial rDNA sequencing

“…Intestinal microbiome is “the second genome” of human. The disturbance of intestinal microorganisms can directly influence immune system, especially inflammatory diseases and autoimmune diseases ( Li et al, 2019 ). Therefore, although genetic factors are proved to be vital to the occurrence of SLE, environmental factors are also highly correlated to SLE.…”

Evaluation of Physicochemical and DPPH� Cleaning Activity of Ultrasonic Assisted Extraction of Polysaccharide from Leonurus japonicus

“…In the cosmetics industry, it is currently being used as facial masks or ointments and also in food products, accompanied with other components to treat skin disease as well as help in activation of blood circulation [3]. Presently, the replacement of synthetic antioxidant with naturally occurring antioxidant is increasing due to the side effects associated with synthetic antioxidant such as increased risk of cancer [4][5][6][7][8][9][10][11][12][13][14][15].…”