Method for accurate diagnose of lupus erythematosus skin lesions based on microbial rDNA sequencing

Zhao, Ying; Zhang, Bin; Liu, Chunyan; Ren, Guohua

doi:10.1016/j.sjbs.2020.05.035

Cited by 7 publications

(4 citation statements)

References 11 publications

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“…Sodium butyrate dampens the pro brotic response induced by TGF-β1 in human dermal broblasts [38]. A study reported higher abundance levels of genus Turicibacter in patients with systemic lupus erythematosus than in healthy individuals [39]. These results are consistent with those of our study and support the existence of a gut-skin axis.…”

Section: Discussionsupporting

confidence: 92%

Impact of the gut microbiome on skin fibrosis: A Mendelian randomisation study

Zhao,

Xu,

et al. 2023

Preprint

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Objectives Skin fibrosis is a lesion in the dermis causing to itching, pain, and psychological stress. The gut microbiome plays as an essential role in skin diseases developments. We conducted a Mendelian randomization study to determine the causal association between the gut microbiome and skin fibrosis. Methods We retrieved valid instrumental variables from the genome-wide association study (GWAS) files of the gut microbiome (n = 18340) conducted by the MiBioGen consortium. Skin fibrosis-associated data were downloaded from the GWAS Catalog. Subsequently, a two-sample Mendelian randomisation (MR) analysis was performed to determine whether the gut microbiome was related to skin fibrosis. A reverse MR analysis was also performed on the bacterial traits which were causally associated with skin fibrosis in the forward MR analysis. In addition, we performed an MR-Pleiotropy Residual Sum and Outlier analysis to remove outliers and a sensitivity analysis to verify our results. Results According to the inverse variance-weighted estimation, we identified that ten bacterial traits (Class Actinobacteria, Class Bacteroidia, family Bifidobacteriaceae, family Rikenellaceae, genus Lachnospiraceae (UCG004 group), genus Ruminococcaceae (UCG013 group), order Bacteroidales, order Bifidobacteriales, genus Peptococcus and genus Victivallis) were negatively correlated with skin fibrosis while five bacterial traits (genus Olsenella, genus Oscillospira, genus Turicibacter, genus Lachnospiraceae (NK4A136group), and genus Sellimonas) were positively correlated. No results were obtained from reverse MR analysis. No significant heterogeneity or horizontal pleiotropy was observed in MR analysis. Conclusion There is a causal association between the gut microbiome and skin fibrosis, indicating the existence of a gut–skin axis. This provides a new breakthrough point for mechanistic and clinical studies of skin fibrosis.

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Section: Discussionsupporting

confidence: 92%

Impact of the gut microbiome on skin fibrosis: A Mendelian randomisation study

Zhao,

Xu,

et al. 2023

Preprint

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“…However, SLE patients in most cohorts both active and remissive, both adults and children all manifested the enrichment of Lactobacillus in both fecal and oral sample in China and Netherlands ( 14 , 15 , 18 , 19 , 46 ) ( Table 1 ). Notably, Lactobacillus enriched in active SLE patients and lupus erythematosus skin lesions patients ( 47 ) as disease severity might play a role in abundance ( 14 ). It is hard to illustrate whether the Lactobacillus correlated with disease severity or higher dose of GC treatment.…”

Section: Lactobacillus Are Altered In Sle Patientsmentioning

confidence: 99%

Lactobacillus: Friend or Foe for Systemic Lupus Erythematosus?

2022

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The cause of Systemic Lupus Erythematosus (SLE) remains largely unknown, despite the fact that it is well understood that a complex interaction between genes and environment is required for disease development. Microbiota serve as activators and are essential to immune homeostasis. Lactobacillus is thought to be an environmental agent affecting the development of SLE. However, beneficial therapeutic and anti-inflammatory effects of Lactobacillus on SLE were also explored. The discovery of Lactobacillus involvement in SLE will shed light on how SLE develops, as well as finding microbiota-targeted biomarkers and novel therapies. In this review, we attempt to describe the two sides of Lactobacillus in the occurrence, development, treatment and prognosis of SLE. We also discuss the effect of different strains Lactobacillus on immune cells, murine lupus, and patients. Finally, we try to illustrate the potential immunological mechanisms of Lactobacillus on SLE and provide evidence for further microbiota-targeted therapies.

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“…52 Several studies have shown that intestinal microorganisms play a key role in the maintenance of immune homeostasis. 53 This study demonstrated that the diversity and composition of the intestinal bacteria had significantly altered in rats with cyclophosphamide-induced immunosuppression but OP treatment reversed those changes. A significant increase in the proportion of Firmicutes and Bacteroidetes was also observed.…”

Section: Discussionmentioning

confidence: 75%

Protective effects of oyster polypeptide on cyclophosphamide‐induced immunosuppressed rats

He,

Nong,

Qin

et al. 2024

J Sci Food Agric

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BackgroundOyster polypeptide (OP) is a mixture of oligopeptides extracted from oysters through enzyme lysis, separation and purification. OP is associated with immunomodulatory effects, but the underlying mechanisms are not known. Therefore, this study combined 1H‐NMR urinary metabolomics and 16S rRNA gene sequencing of the gut microbiome to determine the immunoprotective mechanisms of OP in rats subjected to cyclophosphamide‐induced immunosuppression.ResultsOP can restored the body weight and the structure of spleen and thymus in cyclophosphamide‐induced immunosuppression rats. OP upregulated the levels of white blood cells (WBCs), hemoglobin (HGB), platelets (PLT), red blood cells (RBCs), immunoglobulin G (IgG), immunoglobulin M (IgM), cytokines such as IL‐6 and TNF‐α, and the numbers of CD3+ and CD4+ T cells in the immunosuppression rats. The 1H‐NMR metabolomics results showed that OP significantly reversed the levels of ten metabolites in urinary, including 2‐oxoglutarate, citrate, dimethylamine, taurine, N‐phenylacetylglycine, alanine, betaine, creatinine, uracil, and benzoate. The 16S rRNA gene sequencing results showed that OP restored the gut microbiome homeostasis by increasing the abundance of beneficial bacteria and reducing the abundance of pathogenic bacteria. Finally combining metabolomics and microbiomics found that taurine an hypotaurine metabolism, also alanine, aspartate and glutamate metabolish were disturbed, but these metabolic pathways were restored by OP.ConclusionThis study demonstrated that OP had immunoprotective effects in cyclophosphamide‐induced immunosuppression rats by restoring key metabolic pathways and the gut microbiome homeostasis. Our findings provides a framework for further research into the immunoregulatory mechanisms of OP and its potential use in drugs and nutritional supplements.This article is protected by copyright. All rights reserved.

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Method for accurate diagnose of lupus erythematosus skin lesions based on microbial rDNA sequencing

Cited by 7 publications

References 11 publications

Impact of the gut microbiome on skin fibrosis: A Mendelian randomisation study

Impact of the gut microbiome on skin fibrosis: A Mendelian randomisation study

Lactobacillus: Friend or Foe for Systemic Lupus Erythematosus?

Protective effects of oyster polypeptide on cyclophosphamide‐induced immunosuppressed rats

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