Leonurus japonicus is rich in bioactive compounds especially polysaccharide. Single factors test and response surface methodology were used to study the optimum conditions for ultrasonic - assisted extraction of polysaccharide from L. japonicus, and its physiochemical and DPPH scavenging activities also were evaluated. The optimal conditions as underpinned by this paper is as follow: extraction temperature of 70 �C, extraction time of 50 min and extraction power of 210W. The polysaccharide of L. japonicus was shown to be a non-reducing sugar and to exhibit DPPH scavenging activity to an extent. The polysaccharide structures were studied using SEM, AFM, and FT-IR. It showed L. japonicus polysaccharide had been having more than one chains which were branched and entangled with each other. and existed a broad band wavelength of 2925.64, 1635.06, 1539.7, 1046.73, and 477.86 cm-1. The study indicated that L. japonicus may have a beneficial use to the medical and food industries.
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