Association of Indolebutyric Acid with Azospirillum brasilense in the Rooting of Herbaceous Blueberry Cuttings

Abstract

Association between auxins and plant growth-promoting bacteria can stimulate root growth and development of fruit crop nursery plants, and can be a promising biological alternative to increase the rooting of cuttings. The objective of this study was to assess the viability of producing ‘Powderblue’ blueberry nursery plants from cuttings using different doses of indolebutyric acid (IBA) in association with Azospirillum brasilense. The following treatments were tested: 0 (control); 500 mg L−1 of IBA; 1000 mg L−1 of IBA; A. brasilense; 500 mg L−1 of IBA + A. brasilense; and 1000 mg L−1 of IBA + A. brasilense. The experimental design was completely randomized, with six treatments and four replicates, and each plot (box) consisted of 10 cuttings. The boxes were arranged in a mist chamber with an intermittent regimen controlled by a timer and solenoid valve. After 90 days, the following variables were assessed: rooted cuttings; survival of cuttings; foliar retention; sprouting; cuttings with callus; root dry mass per cutting; number of roots per cutting; and root length. It was observed that the application of IBA with the A. brasilense rhizobacteria increased the number of roots of ‘Powderblue’ blueberry cuttings, while the treatments with IBA alone and IBA 1000 mg L−1 + A. brasilense increased the root length of cuttings. However, treatments with IBA and A. brasilense had no impact on % rooted cuttings and % survival of cuttings.

Rooting of Wild Blueberry (Vaccinium spp.) Cuttings From the Peruvian Northeast

“…Using cuttings for propagation can be an effective strategy to bring wild berries closer to farmers and initiate their cultivation. However, some studies have reported that blueberries have a low rooting rate, which limits their propagation (Braha & Rama, 2018;Koyama et al, 2019). In the current study, the rooting percentages of all five accessions were greater than 90% aer treatment with 2,000 mg L −1 IBA.…”

“…e results showed that the rhizogenic capacity and shoot development of the cuttings varied owing to the influences of the germplasm and IBA concentration. IBA at high concentrations has been reported to potentially have inhibitory effects on vegetative propagation (Koyama et al, 2019), although such effects may vary because of factors such as genetic variation among species or cultivars (An et al, 2018) and variations in the application method (liquid or powder) (Peña et al, 2012) and type of explant (apical, middle, or basal) (An et al, 2018). erefore, for the propagation…”

Efficacy of Different Concentrations of NAA on Selected Ornamental Woody Shrubs Cuttings

“…However, with CCK and CCR, root length was similar or was even inhibited under the same treatment. Another study conducted reveled that indolebutyric acid increased the number of roots and root length of blueberry cuttings, but on the contrary, no effect was observed in rooting percentage and survival percentage of the cuttings [56]. Planting dates and NAA treatments could significantly improve root number and length [57], yet under our experimental conditions, it was determined that planting time did not influence root number or root length.…”

Innovation in Propagation of Fruit, Vegetable and Ornamental Plants

“…The first contribution in this section explored the "Association of Indolebutyric Acid with Azospirillum brasilense in the Rooting of Herbaceous Blueberry Cuttings" by Koyama et al [10]. The use of plant growth-promoting rhizobacteria (PGPRs) can be a promising biological alternative for increasing the rooting of blueberry (Vaccinium sp.)…”