Association of bovine PPARGC1A and OPN genes with milk production and composition in Holstein cattle

Abstract

ABSTRACT:Several studies have reported quantitative trait loci on chromosome 6 affecting milk production and composition traits in dairy cattle. Osteopontin (OPN) and peroxisome proliferator activated receptor-γ coactivator-1α (PPARGC1A) genes have been located on this chromosome and identified as positional candidates for milk traits. We investigated the associations of single nucleotide polymorphism (SNP) T>C at position 1892 and SNP A>C at position 3359 in PPARGC1A gene as well as SNP C>T at position 8514 in OPN gene with milk production and composition. Hence, 398 Iranian Holstein cows were genotyped through polymerase chain reaction and restriction fragment length polymorphism (PCR-RFLP). The Least Squares methods were used to examine the effects of genotypes on milk traits. The frequencies of the C allele at position 1892, A allele at position 3359, and T allele at position 8514 were 0.56, 0.64, and 0.53, respectively. In this study, c.1892T>C genotypes indicated significant associations with milk fat content adjusted for two milkings per day (FAT P2X ; %), estimated breeding value for milk (EBV M ; kg), milk protein yield adjusted for mature body weight (PRO ME ; kg), milk protein yield adjusted for 305 days (PRO 305 ; kg) (P < 0.05), and estimated breeding value for milk fat content (EBV FP ; %) (P < 0.01). There were significant associations between c.3359A>C genotypes and FAT P2X , EBV FP (P < 0.01). Moreover, significant associations were shown between c.8514C>T genotypes in OPN gene and FAT P2X (P < 0.05), and PRO PER305 (P < 0.01). Thus, these SNPs would provide an excellent opportunity for marker assisted selection programs in dairy cattle.

Effect of Osteopontin gene variants on milk production traits in Holstein Friesian crossbred cattle of Kerala

“…Similarly, Boleckova et al [20] also could not find any significant effect for milk yield and fat yield in case of OPN gene variants in Czech Fleckvieh cows. However, the present observations are against the reports of lower milk yield associated with C allele compared with T allele [18,25,26,27].…”

“…In agreement with the present results, Łuczak and Hanna [22] and Kulaj et al [25] also could not derive any significant results for g.8514C > T polymorphism with regard to milk composition traits in Jersey cattle. In contrast, the influence of C allele on protein content was explained in CDDR Holstein [16,18] and Iranian Holstein [20,26,27] populations where significantly higher protein percent was noticed in CC genotyped animals. In the present study, no significant association was found out for FP (P = 0.71).…”

Associations between gene polymorphisms, breeding values, and glucose tolerance test parameters in German Holstein sires

“…The sires with the AA/AA genotype (AA coding for lysine) had high EBVs for fat percentage and Therefore, the EBV for protein yield was higher in sires with GC/GC genotype due to their high EBV for milk yield. DGAT1 is considered to be one of the most important major genes influencing fat percentage, but also other genes are in focus in which significant effects have been found (Pasandideh et al 2015;Shi et al 2016). In this paper, the impact of other polymorphisms on the breeding values was non-significant (P > 0.05) in most cases except for GHR polymorphism and milk fat yield, and OLR1 polymorphism and protein yield (P < 0.05).…”

Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size

“…The reaction volume (25 μl) contained 50 ng of genomic DNA, 12.5 µl of 2 × reaction mix (including 500 µM of each dNTP; 20 mM Tris-HCl, pH 9; 100 mM KCl; and 3 mM MgCl 2 ), 0.5 µM of each primer, and 0.5 U of Taq DNA polymerase (TIANGEN, China). Cycling was performed as follows: 5 min at 95°C, 35 cycles of denaturation at 94°C for 30 s, annealing at X°C (for the values of X see Table 1) for 30 s, extension at 72°C for 35 s, and a final extension at 72°C for 10 min (Pasandideh et al 2015). PCR products (5 μl) obtained using different primer pairs were mixed with 0.7 μl of 10 × RE buffer, 2.5 U of restriction enzyme (NEB, UK), and 3.8 μl of sterilized ddH 2 O.…”