Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size

Abstract

An X., Bao L., Hou J., Bai Y., Zhao X., Song Y., Cao B. . (2017): Two single nucleotide polymorphisms in the caprine GnIH gene are associated with litter size. Czech J. Anim. Sci., 62, 269-275.Gonadotropin-inhibitory hormone (GnIH) can decrease luteinizing hormone and/or follicle-stimulating hormone levels in rat, mouse, sheep, and cattle by the direct suppression of gonadotropin-releasing hormone (GnRH). The present study investigated polymorphisms in the GnIH genes of two dairy goat breeds and their association with litter size. Single nucleotide polymorphisms (SNPs) g.1837C>G and g.3195G>A (GenBank Accession Nos. KR778885 and KR819142) were detected in the GnIH genes of Xinong Saanen and Guanzhong dairy goat breeds using DNA sequencing and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP). Furthermore, the g.1837C>G and g.3195G>A loci were closely linked in both breeds (r 2 > 0.33). Association analysis showed that these SNPs had significant effects on the litter size of goats (P < 0.05). In both breeds, individuals with the CC/GG (g.1837C>G/g.3195G>A) genotype showed larger litter sizes in the second and average parities than individuals with the GG/AA genotype (P < 0.05). Known biochemical and physiological functions, along with our results, indicate that the CC/GG genotype may be used in marker-assisted selection to choose individuals with a larger litter size from both breeds.

Association of a single nucleotide polymorphism (c.-1001A>G) in the gonadotropin inhibitory hormone (GnIH) gene with post-partum anestrus in Indian Murrah buffalo

“…A total of 8 SNPs were identified in the buffalo GnIH/NPVF gene. Among them, 2 SNPs (c.- SNPs were identified in the intron 1 (g.1837C>G) and exon 2 (g.3915G>A) of the GnIH/NPVF gene (An et al 2017). Similarly, five SNPs (g.3305T>C, g.3310T>C, g.3403G>C, g.3411G>A, g3591T>C) were identified in the 3'-UTR region of the chicken GnIH/NPVF (Hu et al 2015).…”

“…Particularly, the genotypes, AG and AA, can explain 4.5% of the variance in PPA of buffaloes that is a great percentage by the genetic differences in any quantitative trait The SNPs in the GnIH/NPVF also showed large genetic effects in reproductive traits in other species. For example, the two SNPs in the intron 1 (g.1837C>G) and exon 2 (g.3915G>A) of the GnIH/NPVF gene in goats were found associated with litter size (An et al 2017). Similarly, among the five SNPs (g.3305 T>C, g.3310T>C, g.3403 G>C, g.3411G>A, g3591T>C) identified in the 3'-UTR region of the chicken GnIH/NPVF, the g.3305 T>C, g.3403 G>C, g.3411G>A were associated with the age at first egg, and the g3591T>C was associated with body weight at the first egg (Hu et al 2015).…”

Genome-wide differences in DNA methylation changes in caprine ovaries between oestrous and dioestrous phases

“…There were around 12 million dairy goats reared in China in 2012 [ 35 ], and Guanzhong dairy goats accounted for around 10% of them. The annual average milk yield of Guanzhong dairy goats is generally 500–600 kg, and their annual average litter size is 1.6–2.0 [ 36 , 37 ]. Ten healthy, 3 years old pluriparous dairy goats were selected based on long-term observations and records of the oestrous cycles in each goat.…”