Association of Adiponectin Gene Polymorphism (+T45G) With Acute Coronary Syndrome and Circulating Adiponectin Levels

Abstract

We investigated the association of adiponectin gene polymorphisms (+T45G and +G276T) and adiponectin levels with acute coronary syndrome (ACS) among Arabs in Qatar. A case-control study was performed in 142 Arab patients with ACS and 122 controls. Genotypes were determined using TaqMan real-time polymerase chain reaction assay. The TT, TG, and GG genotype frequencies of the T45G variant were significantly different among cases and controls (P = .023) but not significant for G276T genotypic frequencies. It was found that only the +45G allele was significantly associated with 3-fold increased risk of ACS (odds ratio = 2.77; 1.03-6.96; P = .043) among patients, using the genetic recessive model. Carriers of GG alleles had significantly lower adiponectin levels compared to TT/TG carriers of T45G in patients with ACS. The present study suggests that only T45G single-nucleotide polymorphism in the adiponectin gene is associated with higher odds for ACS events and has an effect on serum adiponectin levels among Arab populations.

Genetic and Non-Genetic Determinants of Circulating Levels of Adiponectin in a Cohort of Chilean Subjects

“…Adiponectin levels are also influenced by genetic variables; they are considered to be highly heritable, and several single nucleotide polymorphisms (SNPs) in adiponectin-related genes (ADIPOQ, ADIPOR1, ADI-POR2) have been examined in relation to circulating adiponectin levels and obesity phenotypes [8][9][10][11][12][13][14][15][16][17][18][19]. However, the results of these studies have been contradictory with regard to whether variability at this locus has an impact on metabolic phenotypes and which polymorphisms are responsible for such an effect.…”

Adiponectin gene polymorphisms contributes to ischemic stroke risk: A meta-analysis

“…7,8 And a recently published paper suggested the genetic polymorphisms of ADIPOQ were associated with ischemic stroke. [9][10][11][12][13][14][15][16][17][18][19][20][21][22][23][24] In this meta-analysis, a total of eight case-control studies were analyzed to provide a comprehensive assessment of the association between ADIPOQ polymorphisms and ischemic stroke. Genotypes in all studies were detected with genetic DNA from blood samples using a total of three genotyping methods.…”

“…8 Recent evidence has implicated the genetic polymorphism of the adiponectin gene (ADIPOQ) in association with risk of coronary heart disease and ischemic stroke. [9][10][11][12][13][14][15][16][17][18][19][20] Three singlenucleotide polymorphisms (SNPs) including rs266766 (T is converted to G), rs1501299 (G is converted to T) and rs266729 (C is converted to G) were focused on in these studies. However, the conclusions of these studies were inconsistent.…”

Associations of Vitamin D Receptor Polymorphism rs1544410 with Adiposity Phenotypes

“…EDTA-containing tubes were used to collect blood for Genotyping. Measurements of biochemical analysis included the lipid profile, glucose while vitamin D level and insulin were measured by Elisa technique in biomedical lab SciencesQatar University as previously published [23]. The concentration of fasting insulin was performed using the insulin ELISA kit Quantikine ELISA kit (catalog DINS00, USA), and Vitamin D was measured by 25-OH vitamin D (total) ELISA Cat#5396 (DRG Diagnostics, USA).…”

“…The assay-on-demand TM service provided the primers and the probes of these SNPs polymorphisms by (Applied Biosystems). The proper conditions for amplifications were followed by the manufacturer's instructions and negative controls were used, as previously published [23].…”