Association between vascular endothelial growth factor promoter polymorphisms and the risk of recurrent implantation failure

Abstract

Abstract. The objective of the present study was to investigate the association between recurrent implantation failure (RIF) and vascular endothelial growth factor (VEGF) gene polymorphisms that are associated with various female infertility disorders. A total of 116 women diagnosed with RIF and 218 control subjects were genotyped for the VEGF -2578C>A, -1154G>A, -634C>G and 936C>T polymorphisms using a polymerase chain reaction-restriction fragment length polymorphism assay. The VEGF -2578AA genotype was associated with an increased prevalence (≥4) of RIF [adjusted odds ratio (AOR)=2.77; 95% confidence interval (CI)=1.10-7.02; P=0.031], whereas the VEGF -634CG+GG genotype was associated with an increased incidence of total RIF (AOR=2.03; 95% CI=1.02-4.05; P=0.044) and ≥4 RIF (AOR=3.16; 95% CI=1.19-8.37; P=0.021). The results of the haplotype analysis indicated that -2578A/-1154A/-634G/936C (AOR=1.76; 95% CI=1.03-3.00; P=0.040 for total RIF and AOR=2.11; 95% CI=1.12-3.97; P=0.021 for ≥4 RIF) was associated with the occurrence of RIF. In addition, it was revealed that there was a significant difference in serum prolactin level associated with the VEGF -634C>G polymorphism (P=0.013). Therefore the findings of the present study indicate that the VEGF -2578AA genotype, -634G allele and -2578A/-1154A/-634G/936C haplotype may be genetic markers for susceptibility to RIF. However, further studies on VEGF promoter polymorphisms that include an independent randomized-controlled population are required to confirm these results.

Construction of lncRNA-related competing endogenous RNA network and identification of hub genes in recurrent implantation failure

“…As one of the most important angiogenic factors, VEGF plays a vital role in decidual vascularization and placenta angiogenesis. Prior studies also suggest that VEGF polymorphisms are closely associated with the development of RIF [40,41]. Accordingly, we found a significant enrichment of cellular response to VEGF stimulus in the brown module.…”

The ‑2549 insertion/deletion polymorphism in the promoter region of VEGF is associated with the risk of recurrent spontaneous abortion

“…In particular, the -1154 G/A variant was significantly associated with the risk of RSA among non-Asian populations, while the +936 C/T variant was significantly associated with RSA risk among Asian populations (19). In addition, the More recently, Shim et al (40) investigated the association between VEGF promoter polymorphisms -2578 C/A, -1154 G/A, -634 C/G and +936 C/T and RIF. Their findings revealed that the -2578 AA genotype was associated with an increased prevalence of RIF (≥4 implantation failures) compared with the CC genotype, whereas the VEGF -634 CG+GG genotype was associated with an increased incidence of total RIF and ≥4 RIFs compared with the CC genotype.…”

Association study of AGO1 and AGO2 genes polymorphisms with recurrent pregnancy loss

“…An automated photo-optical coagulometer (ACL TOP; Mitsubishi Chemical Medicine, Tokyo, Japan) was used to measure prothrombin time (PT), and activated partial thromboplastin time (aPTT), and a Sysmex XE2100 automated hematology analyzer (Sysmex, Kobe, Japan) was used to determine platelet counts 46 . Hormone levels were measured in one of two ways, depending on the hormone; radioimmunoassays were used to measure estradiol, thyroid-stimulating hormone and prolactin (Beckman Coulter, Inc., Brea, CA, USA), and enzyme immunoassays were used to measure follicle-stimulating hormone and luteinizing hormone (Siemens AG, Munich, Germany) 47 . The proportion of the CD56+ natural killer cells proportion in peripheral blood was determined by flow cytometry (FACS Calibur; BD Biosciences, USA) 48 .…”