The ‑2549 insertion/deletion polymorphism in the promoter region of VEGF is associated with the risk of recurrent spontaneous abortion

Abstract

Abstract. Recurrent spontaneous abortion (RSA) is a common health problem affecting women of reproductive age. Altered expression of vascular endothelial growth factor (VEGF) has been associated with spontaneous abortion. The present case-control study aimed to evaluate the impact of the 18-bp insertion/deletion (ins/del) polymorphism (rs35569394) in the promoter region of the VEGF gene on idiopathic RSA. Genomic DNA from 93 patients with RSA and 93 healthy fertile women of southeastern Iran was isolated using the salting-out method. Genotyping of the rs35569394 variant was performed by a polymerase chain reaction (PCR) method. The findings indicated that the VEGF 18-bp ins/del variant significantly increased the risk of RSA under codominant (ins/ins vs. del/del; OR=2.85, 95% CI=1.31-6.22, P=0.019), dominant (del/ins+ins/ins vs. del/del; OR=2.19, 95% CI=1.20-4.01, P=0.015) and allelic (ins vs. del; OR=1.90, 95% CI=1.25-2.88, P=0.003) inheritance models. In summary, the findings propose a significant association between the VEGF 18-bp ins/del polymorphism and risk of RSA in a sample of the southeast Iranian population. Further studies on larger sample sizes and different ethnicities are required to validate the present findings.

Genetics of recurrent pregnancy loss among Iranian population

“…It has been reported that there was significant high levels of serum VEGF among a subpopulation of Iranian URSA cases (Amirchaghmaghi et al, ). Moreover, another group has reported that the 18‐bp ins/del polymorphism in VEGF significantly increased the risk of RSA in a sample of southeast Iranian cases (Hashemi et al, ). Micro‐RNAs are a class of noncoding RNAs involved in posttranscriptional regulation through mRNA degradation or block of translation (Bartel, ).…”

VEGF gene rs3025039C/T and rs833052C/A variants are associated with bladder cancer risk in Asian descendants

“…12,13 Human VEGF gene spans 14 kb on chromosome 6.p12-p21 and consists of eight exons and seven introns. 14,15 Previous studies showed that VEGF messenger RNA (mRNA) was upregulated in urothelial carcinoma including BCa. 16,17 Furthermore, overexpression of VEGF has also been demonstrated in upper urinary tract urothelial carcinoma and BCa, which could be utilized as a prognostic factor for these cancers.…”

“…Vascular endothelial growth factor (VEGF) is one of the potent endothelial cell‐specific regulators of angiogenesis, which plays a crucial role in the process of tumor growth, invasion, and metastasis . Human VEGF gene spans 14 kb on chromosome 6.p12‐p21 and consists of eight exons and seven introns . Previous studies showed that VEGF messenger RNA (mRNA) was upregulated in urothelial carcinoma including BCa .…”