Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge

Roongsitthichai, Atthaporn; Suwimonteerabutr, Junpen; Kaeoket, Kampon; Koonjaenak, Seri; Tummaruk, Padet

doi:10.1007/s00580-010-1033-2

Cited by 3 publications

(4 citation statements)

References 26 publications

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“…On the contrary, if the pigs were under an influence of estradiol, the function of immune cells would be upregulated [29]. Specifically, the preceding studies revealed that the correlation between serum P 4 concentration and the number of eosinophils was found [5,22]. Likewise, the tendency of positive correlation between serum P 4 concentration and the infiltration of eosinophils in the current study was observed.…”

Section: Discussionsupporting

confidence: 44%

“…"Chronic endometritis" was classified by a plenty of lymphocytes (>40 cells per 20 microscopic fields), together with an existence of plasma cells (>10 cells per 20 microscopic fields), and a rather low number of neutrophils (<20 cells per 20 microscopic fields). "Normal endometrium" was considered when neutrophils were less than 40 cells, lymphocytes were less than 40 cells, and plasma cells were less than 10 cells per 20 microscopic fields [22].…”

Section: Tissue Collectionmentioning

confidence: 99%

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Expression of Cyclooxygenase-2 in the Endometrium of Gilts with Different Stages of Endometritis

Roongsitthichai

Srisuwatanasagul

Koonjaenak

et al. 2011

The Journal of Veterinary Medical Science

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ABSTRACT. The present study determined the association among the expression of COX-2, stages of endometritis, and types and number of local immune cells infiltrating into the gilts' endometrium. The uterine tissues from 24 Landrace x Yorkshire gilts identified as acute endometritis (n=7), chronic endometritis (n=7), and normal endometrium (n=10) were included. The tissues were prepared for both histological and immunohistochemical investigations. The immunoexpression of COX-2 in every layer of the gilts' endometria was appraised by avidin-biotin-peroxidase complex method via image analysis; and was reported as percentage of positive area and staining index. The results revealed that the immunoexpression of COX-2 was found only in the surface epithelial layer. The gilts with acute endometritis possessed higher both percentage of positive area (68.99% versus 4.50% and 3.43%, P<0.001) and staining index (1.13 versus 0.05 and 0.04, P<0.001) than those with chronic endometritis and normal endometrium, respectively. Positive correlations between the number of surface epithelial neutrophils and percentage of COX-2 positive area (r=0.47, P=0.022), as well as mean staining index (r=0.44, P=0.032) were observed. In conclusion, the immunoexpression of COX-2 was found strongest in the gilts with acute endometritis, meanwhile it was not different between those with chronic endometritis and normal endometrium. This suggested that the expression of COX-2 might be dependent not only on the infiltration of local immune cells in the endometrium, but also on the duration of exposure with inflammatory agents.

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Section: Discussionsupporting

confidence: 44%

Section: Tissue Collectionmentioning

confidence: 99%

Expression of Cyclooxygenase-2 in the Endometrium of Gilts with Different Stages of Endometritis

Roongsitthichai

Srisuwatanasagul

Koonjaenak

et al. 2011

The Journal of Veterinary Medical Science

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“…'Chronic endometritis' was categorised by a high number of lymphocytes in the subepithelial connective tissue (>40 cells per 20 microscopic fields with ×400 magnification), the presence of plasma cells in the subepithelial connective tissue layer (>10 cells per 20 microscopic fields with ×400 magnification) and a relatively low number of neutrophils (<20 cells per 20 microscopic fields with ×400 magnification). Endometrium with infiltration of neutrophils, lymphocytes and plasma cells into the subepithelial connective tissue layer being less than 40 cells, 40 cells and 10 cells per 20 microscopic fields with ×400 magnification, respectively, was defined as 'normal endometrium' (Roongsitthichai et al 2011). …”

Section: Gilt Classificationmentioning

confidence: 99%

“…Based on the previous macroscopic and microscopic examination of the gilt's uterus Roongsitthichai et al 2011), the gilts were classified according to both macroscopic and microscopic examination of the endometrium into three groups: normal (n=13), acute endometritis (n=11) and chronic endometritis (n=8). The number of leukocyte subpopulations, including neutrophils, lymphocytes, eosinophils, macrophages and plasma cells, in different layers of the endometrium (surface epithelium, subepithelial connective tissue layer and glandular connective tissue layer) was determined.…”

Section: Gilt Classificationmentioning

confidence: 99%

Porcine circovirus type 2 DNA detection in the uterine tissue of gilts in relation to endometritis and the number of leukocyte subsets in the endometrium

et al. 2015

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The aim of the present study was to determine the prevalence of porcine circovirus type 2 (PCV2) DNA-positive uterine tissues in gilts in relation to endometritis and the number of leukocyte subsets in the endometrium. Uterine tissues from 32 Landrace × Yorkshire gilts were investigated. The gilts were classified according to both macroscopic and microscopic examination of the endometrium into three groups: normal (n=13), acute endometritis (n=11) and chronic endometritis (n=8). Reproductive cycle was defined according to the ovarian appearance as follicular, luteal and inactive phases. PCV2 DNA was determined using polymerase chain reaction. The number of leukocyte subsets, including lymphocytes, neutrophils, eosinophils, macrophages and plasma cells, in the epithelium, subepithelium and glandular layer of the endometrium was carefully evaluated. It was found that PCV2 DNA was detected in 34.4 % (11/32) of the uteri. According to the lesion of endometritis, PCV2 DNA was detected in 53.9 % (7/13) of normal uteri, 36.4 % (4/11) of acute endometritis, and 0.0 % (0/8) of chronic endometritis (P= 0.038). The PCV2 DNA was detected in 55.0 % (5/9), 20.0 % (4/20) and 66.7 % (2/3) of tissues in follicular, luteal and inactive phases, respectively (P=0.081). Leukocyte subsets in all tissue layers of the endometrium did not differ significantly between the uterine tissues with and without PCV2 DNA detection (P>0.05). These findings indicate that PCV2 exists in the reproductive organs of the gilts. The PCV2 persistence can be a risk factor for transmission of the virus to negative sows and potentially cause reproductive failures.Nevertheless, the influence of PCV2 natural infection on the reproductive function of gilt still requires further investigation.

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Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand

Pearodwong

Srisuwatanasagul

Teankum

et al. 2015

Trop Anim Health Prod

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Association between serum cortisol and progesterone concentrations and the infiltration of immune cells in the endometrium of gilts with vaginal discharge

Cited by 3 publications

References 26 publications

Expression of Cyclooxygenase-2 in the Endometrium of Gilts with Different Stages of Endometritis

Expression of Cyclooxygenase-2 in the Endometrium of Gilts with Different Stages of Endometritis

Porcine circovirus type 2 DNA detection in the uterine tissue of gilts in relation to endometritis and the number of leukocyte subsets in the endometrium

Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand

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