Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand

Pearodwong, Pachara; Srisuwatanasagul, Sayamon; Teankum, Komkrich; Tantilertcharoen, Rachod; Tummaruk, Padet

doi:10.1007/s11250-015-0796-5

Cited by 5 publications

(8 citation statements)

References 22 publications

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“…The immunohistochemistry protocol was carried out according to a previous study (Pearodwong et al 2015). Briefly, 4-μm-thick sections were deparaffinised with xylene and rehydrated with graded ethanol.…”

Section: Dna Extraction and Pcv2 Detectionmentioning

confidence: 99%

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Porcine circovirus type 2 DNA detection in the uterine tissue of gilts in relation to endometritis and the number of leukocyte subsets in the endometrium

et al. 2015

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The aim of the present study was to determine the prevalence of porcine circovirus type 2 (PCV2) DNA-positive uterine tissues in gilts in relation to endometritis and the number of leukocyte subsets in the endometrium. Uterine tissues from 32 Landrace × Yorkshire gilts were investigated. The gilts were classified according to both macroscopic and microscopic examination of the endometrium into three groups: normal (n=13), acute endometritis (n=11) and chronic endometritis (n=8). Reproductive cycle was defined according to the ovarian appearance as follicular, luteal and inactive phases. PCV2 DNA was determined using polymerase chain reaction. The number of leukocyte subsets, including lymphocytes, neutrophils, eosinophils, macrophages and plasma cells, in the epithelium, subepithelium and glandular layer of the endometrium was carefully evaluated. It was found that PCV2 DNA was detected in 34.4 % (11/32) of the uteri. According to the lesion of endometritis, PCV2 DNA was detected in 53.9 % (7/13) of normal uteri, 36.4 % (4/11) of acute endometritis, and 0.0 % (0/8) of chronic endometritis (P= 0.038). The PCV2 DNA was detected in 55.0 % (5/9), 20.0 % (4/20) and 66.7 % (2/3) of tissues in follicular, luteal and inactive phases, respectively (P=0.081). Leukocyte subsets in all tissue layers of the endometrium did not differ significantly between the uterine tissues with and without PCV2 DNA detection (P>0.05). These findings indicate that PCV2 exists in the reproductive organs of the gilts. The PCV2 persistence can be a risk factor for transmission of the virus to negative sows and potentially cause reproductive failures.Nevertheless, the influence of PCV2 natural infection on the reproductive function of gilt still requires further investigation.

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Section: Dna Extraction and Pcv2 Detectionmentioning

confidence: 99%

“…Nevertheless, PCV2 DNA has been detected in the uterine tissue of the gilts culled due to purulent vaginal discharge and anoestrous problems (Pearodwong et al 2015). The culling rate of sows and gilts in commercial swine herds varies from 40 to 50 % annually .…”

Section: Introductionmentioning

confidence: 99%

Porcine circovirus type 2 DNA detection in the uterine tissue of gilts in relation to endometritis and the number of leukocyte subsets in the endometrium

et al. 2015

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“…First evidence of their existence was their detection in a porcine kidney cell line (PK15) leading to the name porcine circovirus type 1 (Tischer et al, 1974). More than two decades later a second porcine circovirus, PCV2, was detected and made responsible for a couple of syndromes including post weaning multisystemic wasting syndrome, porcine dermatitis and nephropathy syndrome and PCV2-associated reproductive failure which were summarized later under the so-called porcine circovirus associated disease (PCVAD) (Limsaranrom et al, 2015;Meng, 2012;Pearodwong et al, 2015;Shin et al, 2015). Currently four genotypes namely PCV2a, PCV2b, PCV2c and PCV2d-mPCV2b are recognized (Franzo et al, 2015).…”

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Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs

Herbst

Willems

2017

Research in Veterinary Science

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During routine electron microscopy of fecal samples from diarrheic dogs dated from 2000 virus particles resembling circovirus in shape and size were detected in two samples (V2177/00; V3374/00). Polymerase chain reaction (PCR) using primers specific for porcine circovirus type 2 (PCV2) amplified DNA recovered from both samples. Sequencing of PCR amplificates (V2177/00) obtained with PCV2-specific primer pairs revealed a genome size of 1768bp. The nucleotide sequence was highly similar (98% nucleotide identity) to the PCV2a reference sequence.

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“…In recent years, PCV2 has been associated with many disease manifestations in all ages of pigs, so-called PCV2-associated disease (PCVAD) [ 8 ]. PCV2 is a primary cause of post-weaning multisystemic wasting syndrome, porcine dermatitis and nephropathy syndrome and PCV2-associated reproductive failure [ 2 , 9 , 14 ]. PCV2-associated disease has a significant economic impact on the swine industry worldwide.…”

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confidence: 99%

“…The paraffin embedded ovarian tissues were cut into 4 µ m thick sections and were divided into two groups according to previously identified PCV2 DNA detection, i.e. the PCV2-infected gilts (n=6) and non-infected gilts (n=5) [ 9 ]. Historical data and blood samples were collected from the gilts before culling.…”

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Expression of PCV2 antigen in the ovarian tissues of gilts

Tummaruk

Pearodwong

2016

The Journal of Veterinary Medical Science

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The present study was performed to determine the expression of porcine circovirus type 2 (PCV2) antigen in the ovarian tissue of naturally infected gilts. Ovarian tissues were obtained from 11 culled gilts. The ovarian tissues sections were divided into two groups according to PCV2 DNA detection using PCR. PCV2 antigen was assessed in the paraffin embedded ovarian tissue sections by immunohistochemistry. A total of 2,131 ovarian follicles (i.e., 1,437 primordial, 133 primary, 353 secondary and 208 antral follicles), 66 atretic follicles and 131 corpora lutea were evaluated. It was found that PCV2 antigen was detected in 280 ovarian follicles (i.e., 239 primordial follicles, 12 primary follicles, 10 secondary follicles and 19 antral follicles), 1 atretic follicles and 3 corpora lutea (P<0.05). PCV2 antigen was detected in primordial follicles more often than in secondary follicles, atretic follicles and corpora lutea (P<0.05). The detection of PCV2 antigen was found mainly in oocytes. PCV2 antigen was found in both PCV2 DNA positive and negative ovarian tissues. It can be concluded that PCV2 antigen is expressed in all types of the ovarian follicles and corpora lutea. Further studies should be carried out to determine the influence of PCV2 on porcine ovarian function and oocyte quality.

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Prevalence of porcine circovirus-2 DNA-positive ovarian and uterine tissues in gilts culled due to reproductive disturbance in Thailand

Cited by 5 publications

References 22 publications

Porcine circovirus type 2 DNA detection in the uterine tissue of gilts in relation to endometritis and the number of leukocyte subsets in the endometrium

Porcine circovirus type 2 DNA detection in the uterine tissue of gilts in relation to endometritis and the number of leukocyte subsets in the endometrium

Detection of virus particles resembling circovirus and porcine circovirus 2a (PCV2a) sequences in feces of dogs

Expression of PCV2 antigen in the ovarian tissues of gilts

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