Association between polymorphisms in exons 4 and 10 of the BPI gene and immune indices in Sutai pigs

Wu, Zhengchang; Liu, Y.; Zhao, Qiaohui; Zhu, Shuang; Huo, Yongjiu; Zhu, Guoqiang; Wu, Shenglong; Bao, Wenbin

doi:10.4238/2015.june.8.2

Cited by 5 publications

(4 citation statements)

References 13 publications

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“…In this study, the polymorphism distribution of exon 10 in the BPI gene was detected in Meishan pigs. There were 3 genotypes, of which A was the predominant allele, which is consistent with the results of Wu et al (15). The suitability test results showed that HpaII polymorphisms of exon 10 in the BPI gene deviated from Hardy-Weinberg equilibrium (P < 0.01).…”

Section: Discussionsupporting

confidence: 90%

Polymorphisms of exon 10 in the BPI gene and its association analysis with some reproductive traits and partial immune indexes in Meishan pigs

Qu¹,

Wei²,

Ye³

et al. 2019

Turk J Vet Anim Sci

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This study was conducted to detect polymorphisms of BPI gene exon 10 by PCR-RFLP in Meishan pigs and their associations with some important cytokine levels (IL-1β, IL-4, IL-6, IL-8, IL-10, TGF-1β, IFN-γ, TNF-α) and reproductive traits (total number born (TNB), number born alive (NBA), birth litter weight (BLW), and weaning litter weight (WLW)), with the aim of identifying effective genetic markers for molecular breeding. The results revealed three genotypes, including AA, AB, and BB, with the trend of AA > BB > AB in reproductive traits. Pigs with the AA genotype were significantly higher than the AB genotype in TNB and IFN-γ (P < 0.05), and extremely significantly higher in NBA and WLW (P < 0.01). Moreover, TGF-1β and TNF-α levels were significantly higher in the AA genotype than the BB genotype (P < 0.05). However, there were no significant differences among the three genotypes in the levels of other cytokines (P > 0.05). Comprehensive analysis indicated that the polymorphisms of exon 10 in the BPI gene have significant genetic effects on some reproductive traits and cytokines in Meishan pigs, for which the AA genotype was demonstrated to be the favorable genotype, which could be used as potential genetic markers for in-depth research and examination.

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Section: Discussionsupporting

confidence: 90%

Polymorphisms of exon 10 in the BPI gene and its association analysis with some reproductive traits and partial immune indexes in Meishan pigs

Qu¹,

Wei²,

Ye³

et al. 2019

Turk J Vet Anim Sci

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“…Studies investigating the cytokines Interferon (IFN)- γ and IL-10 used vaccination with Mycoplasma hyopneumoniae , tetanus toxoid, the porcine reproductive and respiratory syndrome virus (PRRSV) [ 39 , 40 ] and the classical swine fever virus [ 41 , 42 ]. Another study analysing cytokines, including IL-6 and IL-12, was restricted to exonic regions of a single gene [ 43 ].…”

Section: Introductionmentioning

confidence: 99%

Genome-wide associations for immune traits in two maternal pig lines

et al. 2021

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Background In recent years, animal welfare and health has become more and more important in pig breeding. So far, numerous parameters have been considered as important biomarkers, especially in the immune reaction and inflammation. Previous studies have shown moderate to high heritabilities in most of these traits. However, the genetic background of health and robustness of pigs needs to be extensively clarified. The objective of this study was to identify genomic regions with a biological relevance for the immunocompetence of piglets. Genome-wide Association Studies (GWAS) in 535 Landrace (LR) and 461 Large White (LW) piglets were performed, investigating 20 immune relevant traits. Besides the health indicators of the complete and differential blood count, eight different cytokines and haptoglobin were recorded in all piglets and their biological dams to capture mediating processes and acute phase reactions. Additionally, all animals were genotyped using the Illumina PorcineSNP60v2 BeadChip. Results In summary, GWAS detected 25 genome-wide and 452 chromosome-wide significant SNPs associated with 17 immune relevant traits in the two maternal pig lines LR and LW. Only small differences were observed considering the maternal immune records as covariate within the statistical model. Furthermore, the study identified across- and within-breed differences as well as relevant candidate genes. In LR more significant associations and related candidate genes were detected, compared with LW. The results detected in LR and LW are partly in accordance with previously identified quantitative trait loci (QTL) regions. In addition, promising novel genomic regions were identified which might be of interest for further detailed analysis. Especially putative pleiotropic regions on SSC5, SSC12, SSC15, SSC16 and SSC17 are of major interest with regard to the interacting structure of the immune system. The comparison with already identified QTL gives indications on interactions with traits affecting piglet survival and also production traits. Conclusion In conclusion, results suggest a polygenic and breed-specific background of immune relevant traits. The current study provides knowledge about regions with biological relevance for health and immune traits. Identified markers and putative pleiotropic regions provide first indications in the context of balancing a breeding-based modification of the porcine immune system.

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“…IL-12 has extensive capacity to activate cytotoxic lymphocytes, including Th1-mediated CD4+ cell differentiation, and stimulates the activation of natural killer (NK) cells and the production of INF-γ 43 ; therefore, it has been considered a target gene for bioprevention. Wu et al 44 demonstrated that the higher expression of IL-6 or IL-12 contributed to improving the general disease resistance in pigs 44 . Thus, we speculated the CD14 gene probably represents an important candidate gene related to the resistance to E. coli F18 in Chinese Meishan pigs.…”

Section: Discussionmentioning

confidence: 99%

CD14 in the TLRs signaling pathway is associated with the resistance to E. coli F18 in Chinese domestic weaned piglets

Liu

Dong

et al. 2016

Sci Rep

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Escherichia coli F18 (E. coli F18) is mainly responsible for post-weaning diarrhea (PWD) in piglets. The genetic basis and regulatory mechanism of E. coli F18 resistance in Chinese domestic weaned piglets remain unclear. Meishan piglets were used as model animals to test their susceptibility to E. coli F18. By performing a comparative transcriptome study on duodenum tissues of sensitive and resistant pigs, we identified 198 differentially expressed genes (DEGs; 125 upregulated and 73 downregulated) in the resistant pigs. DEGs were predominately involved in immune system pathways, including the Toll-like receptor (TLR) signaling pathway. qPCR and western blot showed CD14, IFN-α, TLR4 and IL-1β, etc. in the TLR signaling pathway had significantly higher expression levels in lipopolysaccharide (LPS)-induced small intestinal epithelial cell lines (IPEC-J2) than those in normal IPEC-J2 cells. Immunohistochemical analysis showed the increased expression of CD14 gene in the E. coli F18-resistant individuals. After CD14 knockdown, the levels of cytokines IL-6 and IL-12 were significantly reduced in IPEC-J2 cell supernatants. The adhesion ability of F18ab strain with IPEC-J2 cells was significantly increased (p < 0.01). This study revealed the TLR signaling pathway, and especially CD14, probably plays an important role in resistance to E. coli F18 infection in Chinese domestic piglets.

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Association between polymorphisms in exons 4 and 10 of the BPI gene and immune indices in Sutai pigs

Cited by 5 publications

References 13 publications

Polymorphisms of exon 10 in the BPI gene and its association analysis with some reproductive traits and partial immune indexes in Meishan pigs

Polymorphisms of exon 10 in the BPI gene and its association analysis with some reproductive traits and partial immune indexes in Meishan pigs

Genome-wide associations for immune traits in two maternal pig lines

CD14 in the TLRs signaling pathway is associated with the resistance to E. coli F18 in Chinese domestic weaned piglets

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