Association between Circulating MicroRNAs (miR-21-5p, miR-20a-5p, miR-29b-3p, miR-126-3p and miR-101-3p) and Chronic Allograft Dysfunction in Renal Transplant Recipients

Abstract

Chronic allograft dysfunction (CAD) is a major condition affecting long-term kidney graft survival. Serum microRNA (miRNA) has been reported as a biomarker for various conditions of allograft injuries. The upregulation of miR-21 is the best-known miRNA change in graft tissue, urine and plasma. However, the correlation of plasma miR-21 with the severity of CAD remains unclear. In our study, 40 kidney transplantation recipients with late graft survival for more than 10 years were enrolled. The CAD group (n = 20) had either an eGFR between 15 to 60 mL/min or a biopsy-proved chronic allograft nephropathy or rejection. The control group (n = 20) had an eGFR ≥ 60 mL/min without proteinuria and hematuria for a consecutive 3 months before the study. We performed RNA sequencing to profile the miRNAs expression. There were six differentially expressed miRNAs in the CAD group. Among them, miR-21-5p and miR-101-3p were decreased, and miR-20a-5p was increased. We found that miR-21-5p, miR-20a-5p and miR-101-3p all participated in the TGF-beta pathway. We demonstrated that decreased miR-21-5p and miR-101-3p, and increased miR-20a-5p were the novel CAD-associated miRNAs in the TGF-beta pathway. These findings may pave the way for developing early prediction miRNAs biomarkers for CAD, and possibly developing therapeutic tools in the field of kidney transplantation.

Dysregulation of MicroRNAs in Adult Osteogenesis Imperfecta: The miROI Study

“…Some studies suggest that let-7g-5p may participate in osteoblastic differentiation, but the mechanisms are not yet well understood, with a more oncogenic role in extraosseous bone. [68][69][70][71] MiR-15b-5p that was overexpressed in sera of OI patients in miRNome analysis was weakly but significantly downregulated in OI patients in the validation phase (p < 0.042) and did not differ in quantitative and structural groups. MiR-15b-5p is known for its dual role in oncogenesis as an oncogene or a tumor suppressor, depending on its dysregulation in various tissue and of its numerous gene targets.…”

New Insights into Pediatric Kidney Transplant Rejection Biomarkers: Tissue, Plasma and Urine MicroRNAs Compared to Protocol Biopsy Histology

“…Its overexpression positively correlates with acute kidney injury in patients with multiorgan failure [40]. Recently, circulating mir-101-3p was associated with biopsy-proven chronic allograft nephropathy or rejection in a cohort of adults with ten-year-old transplants [41].…”

Common miRNAs of Osteoporosis and Fibromyalgia: A Review

“…Concerning the seven miRNAs regulated in the same direction (up or down), all cohorts in FM patients were around 50 years old, except one [28] that did not mention the age. In OP, the patients were over 50 years old, with 70-year-old persons in five studies [23,36,37,41,43] and no mention of age in several [38][39][40]. In FM, all studies but one [32]-with only 30% men-included only females.…”

“…One study included both genders, with 60% women [42]. The remaining two studies did not mention gender [39,41].…”