Dysregulation of MicroRNAs in Adult Osteogenesis Imperfecta: The miROI Study

Abstract

As epigenetic regulators of gene expression, circulating microRNAs (miRNAs) have been described in several bone diseases as potential prognostic markers. The aim of our study was to identify circulating miRNAs potentially associated with the severity of Osteogenesis Imperfecta (OI), in 3 steps. We have screened by RNA sequencing for the microRNAs (miRNAs) that were differentially expressed in sera of a small group of OI patients versus controls and then conducted a validation phase by RT‐qPCR analysis of sera of a larger patient population. In the first phase of miROI, we found 79 miRNAs that were significantly differentially expressed. We therefore selected 19 of them as the most relevant. In the second phase, we were able to validate the significant overexpression of 8 miRNAs in the larger OI group. Finally, we looked for a relationship between the level of variation of the validated miRNAs and the clinical characteristics of OI. We found a significant difference in the expression of two microRNAs in those patients with Dentinogenesis Imperfecta. After reviewing the literature, we found 6 of the 8 miRNAs already known to have a direct action on bone homeostasis. Furthermore, the use of a miRNA‐gene interaction prediction model revealed a 100% probability of interaction between 2 of the 8 confirmed miRNAs and COL1A1 and/or COL1A2. This is the first study to establish the miRNA signature in OI, showing a significant modification of miRNAs expression potentially involved in the regulation of genes involved in the physiopathology of OI.This article is protected by copyright. All rights reserved.