Assisted reproduction in HIV-1-serodifferent couples

Leruez‐Ville, Marianne; Almeida, Marta; Tachet, Anne; Dulioust, Emmanuel; Guibert, J.; Mandelbrot, Laurent; Salmon, Dominique; Jouannet, Pierre; Rouzioux, Christine

doi:10.1097/00002030-200211220-00006

Cited by 51 publications

(3 citation statements)

References 22 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Importantly, our data indicate that early TGCs remained viable in culture after viral entry, integration, and protein expression, suggesting that viral integration was not detrimental to these cells. Clonal infection of the male germ line up to spermatozoa is suggested by reports of HIV DNA in a low percentage of ejaculated sperm (16)(17)(18)(19)(20)(21)23). Indeed, spermatozoa do not support HIV entry, are transcriptionally silent, and have a highly compact chromatin, thus preventing HIV replication steps.…”

Section: Discussionmentioning

confidence: 99%

“…We and others found that HIV/SIV strains in semen are locally produced in a subset of individuals (reviewed in reference 13) and arise from several organs within the male genital tract (14). Interestingly, HIV-1 can associate with spermatozoa in vitro (15) and was detected by some authors in a small proportion of patients' sperm (16)(17)(18)(19)(20)(21)(22)(23)(24)(25). Since spermatozoa have a highly compact nucleus and are transcriptionally silent, this detection may suggest infection of their germ cell progenitors in the testis.…”

mentioning

confidence: 99%

See 1 more Smart Citation

Potential for Virus Endogenization in Humans through Testicular Germ Cell Infection: the Case of HIV

Mahé

Matusali

Deléage

et al. 2020

J Virol

View full text Add to dashboard Cite

Viruses have colonized the germ line of our ancestors at several occasions during evolution, leading to the integration in the human genome of viral sequences from over 30 retroviral groups and a few non-retroviruses. Among the recently emerged viruses infecting humans, several target the testis (eg HIV, Zika and Ebola viruses). Here we aimed to investigate whether human testicular germ cells (TGCs) can support integration by HIV, a contemporary retrovirus that started to spread in the human population during the last century. We report that albeit alternative receptors enabled HIV-1 binding to TGCs, HIV virions failed to infect TGCs in vitro. Nevertheless, exposure of TGCs to infected lymphocytes, naturally present in the testis from HIV+ men, led to HIV-1 entry, integration and early protein expression. Similarly, cell-associated infection or bypassing viral entry led to HIV-1 integration in a spermatogonial cell line. Using DNAscope, HIV-1 and SIV DNA were detected within a few TGCs in the testis from one infected patient, one rhesus macaque and one African Green monkey in vivo. Molecular landscape analysis revealed that early TGCs were enriched in HIV early co-factors up to integration and had overall low antiviral defenses when compared with testicular macrophages and Sertoli cells. In conclusion, our study reveals that TGCs can support the entry and integration of HIV upon cell-associated infection. This could represent a way for this contemporary virus to integrate our germline and become endogenous in the future, as happened during human evolution for a number of viruses. IMPORTANCE Viruses have colonized the host germ line at many occasions during evolution to eventually become endogenous. Here we aimed at investigating whether human testicular germ cells (TGCs) can support such viral invasion by studying HIV interactions with TGCs in vitro. Our results indicate that isolated primary TGCs express alternative HIV-1 receptors allowing virions binding but not entry. However, HIV-1 entered and integrated in TGCs upon cell-associated infection, and produced low level of viral proteins. In vivo, HIV-1 and SIV DNA was detected in a few TGCs. Molecular landscape analysis showed that TGCs have overall weak antiviral defenses. Altogether, our results indicate that human TGCs can support HIV-1 early replication including integration, suggesting potential for endogenization in the future generations.

show abstract

Section: Discussionmentioning

confidence: 99%

mentioning

confidence: 99%

Potential for Virus Endogenization in Humans through Testicular Germ Cell Infection: the Case of HIV

Mahé

Matusali

Deléage

et al. 2020

J Virol

View full text Add to dashboard Cite

show abstract

“…Sperm parameters that reflect fertility are significantly impaired in HIV-1 infected men. Measurements including semen volume, sperm motility, concentration and morphology are adversely affected (88–90). Semen parameters correlate positively with CD4 counts (91), which suggests that patients with full blown AIDS are less fertile than healthier HIV-1 infected males.…”

Section: Male Infertility and Hiv/aidsmentioning

confidence: 99%

Human immunodeficiency virus/acquired immunodeficiency syndrome and infertility: emerging problems in the era of highly active antiretrovirals

Kushnir

Lewis

2011

Fertility and Sterility

View full text Add to dashboard Cite

Objective To review the effects of HIV/AIDS, associated co-morbid conditions, and side effects of antiretroviral treatment on fertility. Design A Pubmed computer search was performed to identify relevant articles. Setting Research institution. Intervention(s) None. Result(s) Biological alterations in reproductive physiology may account for sub-fertility in patients infected with HIV. Psychosocial factors in patients with HIV infection may affect reproductive desires and outcomes. Antiretroviral medications may have direct toxicity on gametes and embryos. Available evidence indicates that fertility treatments can be a safe option for HIV-discordant couples; although, potential risk of viral transmission cannot be completely eliminated. Conclusion(s) Reproductive desires are increasingly becoming prominent in the healthcare of young HIV-infected patients. Additional data is needed to address the effect of HIV and its treatments on fertility and reproductive outcomes.

show abstract

Multicenter quality control of the detection of HIV-1 genome in semen before medically assisted procreation

Pasquier¹,

Anderson

Andreutti-Zaugg

et al. 2006

J. Med. Virol.

Self Cite

View full text Add to dashboard Cite

Couples in whom the man is HIV-1-positive may use medically assisted procreation in order to conceive a child without contaminating the female partner. But, before medically assisted procreation, the semen has to be processed to exclude HIV and tested for HIV nucleic acid before and after processing. The performance was evaluated of the technical protocols used to detect and quantify HIV-1 in 11 centers providing medically assisted procreation for couples with HIV-1 infected men by testing panels of seminal plasma and cells containing HIV-1 RNA and/or DNA. The performance of these tests varied due to the different assays used. False positive results were obtained in 14-19% of cases. The sensitivity for RNA detection in seminal plasma was 500-1,000 RNA copies/ml, over 500 RNA copies/10(6) cells in semen cells, and for DNA detection in semen cells 50-500 DNA copies/10(6) cells. The use of silica-based extraction seemed to increase the assay performance, whereas the use of internal controls to detect PCR inhibitor did not. This first quality control highlights the need for technical improvements of the assays to detect and quantify HIV in semen fractions and for regular evaluation of their performance.

show abstract

Assisted reproduction in HIV-1-serodifferent couples

Abstract: Selected spermatozoa may be positive for HIV RNA detection even in treated patients. Viral validation of processed semen is necessary in ART programmes for serodifferent couples, particularly in men with only partially or poorly controlled HIV infection.

Cited by 51 publications

References 22 publications

Potential for Virus Endogenization in Humans through Testicular Germ Cell Infection: the Case of HIV

Potential for Virus Endogenization in Humans through Testicular Germ Cell Infection: the Case of HIV

Human immunodeficiency virus/acquired immunodeficiency syndrome and infertility: emerging problems in the era of highly active antiretrovirals

Multicenter quality control of the detection of HIV-1 genome in semen before medically assisted procreation

Contact Info

Product

Resources

About