“…Oligonucleotide primer pairs for four microsatellite regions containing (CA)n dinucleotide repeat (D11S935, D11S905, D11S934, D11S912) [23] were synthesized on a DNA synthesizer (Applied Biosystems, Foster City, CA, USA). The oligonucleotide primer was end-labeled with [r-32 P]adenosine 5Ј-triphosphates (Amersham, Tokyo, Japan) at 3,000 Ci/mmol using T4 polynucleotide kinase (New England Biolabs, Beverly, MA, USA), followed by the removal of unincorporated 32 P by using a spin column.…”