A 1.4-Mb High-Resolution Physical Map and Contig of Chromosome Segment 11p15.5 and Genes in the LOH11A Metastasis Suppressor Region

Bepler, Gerold; O'Briant, Kathy; Kim, Young Chul; Schreiber, G; Pitterle, Diana M.

doi:10.1006/geno.1998.5659

Cited by 28 publications

(29 citation statements)

References 55 publications

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“…First, regions that appear to be gene-rich appear to be incompatible with YAC cloning due to their high GC content (Boycott et al, 1998). Second, regions with large numbers of repetitive elements have also been found to be difficult to clone in both YACs and BACs (Bepler et al, 1999). Since the D2S123-D2S2251 region is gene-poor, this latter explanation appears to be more likely.…”

Section: Construction Of the Yac-bac Map Of 2p15-p21mentioning

confidence: 92%

Genomic Mapping of Chromosomal Region 2p15–p21 (D2S378–D2S391): Integration of Genemap'98 within a Framework of Yeast and Bacterial Artificial Chromosomes

et al. 1999

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Section: Construction Of the Yac-bac Map Of 2p15-p21mentioning

confidence: 92%

Genomic Mapping of Chromosomal Region 2p15–p21 (D2S378–D2S391): Integration of Genemap'98 within a Framework of Yeast and Bacterial Artificial Chromosomes

et al. 1999

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“…Furthermore, despite the recent publications of working draft human genome sequences [International Human Genome Sequencing Consortium, 2001;Venter et al, 2001] and an almost-finished sequence of about 385 kb encompassing HBBC in human [Bulger et al, 2000], the region between HBB and some of the large deletion breakpoints has not been filled. This region has been resistant to multiple efforts at sequencing [Bulger et al, 2000], and it appears to rearrange frequently in large genomic clones [Bepler et al, 1999;Imam et al, 2000]. Thus it may fall into the unsequenceable class of genomic DNA, and a continuous reference sequence may not be available for some time (if ever).…”

Section: Examples Of Usementioning

confidence: 98%

HbVar: A relational database of human hemoglobin variants and thalassemia mutations at the globin gene server

et al. 2002

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We have constructed a relational database of hemoglobin variants and thalassemia mutations, called HbVar, which can be accessed on the web at http://globin.cse.psu.edu. Extensive information is recorded for each variant and mutation, including a description of the variant and associated pathology, hematology, electrophoretic mobility, methods of isolation, stability information, ethnic occurrence, structure studies, functional studies, and references. The initial information was derived from books by Dr. Titus Huisman and colleagues [Huisman et al., 1996, 1997, 1998]. The current database is updated regularly with the addition of new data and corrections to previous data. Queries can be formulated based on fields in the database. Tables of common categories of variants, such as all those involving the alpha1-globin gene (HBA1) or all those that result in high oxygen affinity, are maintained by automated queries on the database. Users can formulate more precise queries, such as identifying "all beta-globin variants associated with instability and found in Scottish populations." This new database should be useful for clinical diagnosis as well as in fundamental studies of hemoglobin biochemistry, globin gene regulation, and human sequence variation at these loci.

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“…Genetically, HBB locates at 11p15.5 (Bepler et al, 1999) and this locus contains embryonic and adult beta-like globin genes that are ordered as they are expressed during development. High-level expression of the beta-globin gene cluster is regulated by the locus control region (LCR) (Sawado et al, 2003).…”

Section: Discussionmentioning

confidence: 99%

Decreased expression of haemoglobin beta (HBB) gene in anaplastic thyroid cancer and recovory of its expression inhibits cell growth

et al. 2005

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Anaplastic thyroid cancer (ATC) is one of the most fulminant and foetal diseases in human malignancies. However, the genetic alterations and carcinogenic mechanisms of ATC are still unclear. Recently, we investigated the gene expression profile of 11 anaplastic thyroid cancer cell lines (ACL) and significant decreased expression of haemoglobin beta (HBB) gene in ACL. Haemoglobin beta is located at 11p15.5, where loss of heterozygosity (LOH) was reported in various kinds of cancers, including ATC, and it has been suggested that novel tumour suppressor genes might exist in this region. In order to clarify the meaning of decreased expression of HBB in ATC, the expression status of HBB was investigated with ACL, ATC, papillary thyroid cancer (PTC) and normal human tissues. Haemoglobin beta showed significant decreased expression in ACLs and ATCs; however, in PTC, HBB expressed equal to the normal thyroid gland. In addition, HBB expressed in normal human tissues ubiquitously. To validate the tumour-suppressor function of HBB, cell growth assay was performed. Forced expression of HBB in KTA2 cell, which is a kind of ACL, significantly suppressed KTA2 growth. The mechanism of downregulation of HBB in ATC is still unclear; however, our results suggested the possibility of HBB as a novel tumour-suppressor gene.

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A 1.4-Mb High-Resolution Physical Map and Contig of Chromosome Segment 11p15.5 and Genes in the LOH11A Metastasis Suppressor Region

Cited by 28 publications

References 55 publications

Genomic Mapping of Chromosomal Region 2p15–p21 (D2S378–D2S391): Integration of Genemap'98 within a Framework of Yeast and Bacterial Artificial Chromosomes

Genomic Mapping of Chromosomal Region 2p15–p21 (D2S378–D2S391): Integration of Genemap'98 within a Framework of Yeast and Bacterial Artificial Chromosomes

HbVar: A relational database of human hemoglobin variants and thalassemia mutations at the globin gene server

Decreased expression of haemoglobin beta (HBB) gene in anaplastic thyroid cancer and recovory of its expression inhibits cell growth

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