Assessment on induced genetic variability and divergence in the mutagenized lentil populations of microsperma and macrosperma cultivars developed using physical and chemical mutagenesis

Abstract

Induced mutagenesis was employed to create genetic variation in the lentil cultivars for yield improvement. The assessments were made on genetic variability, character association, and genetic divergence among the twelve mutagenized populations and one parent population of each of the two lentil cultivars, developed by single and combination treatments with gamma rays and hydrazine hydrates. Analysis of variance revealed significant inter-population differences for the observed quantitative phenotypic traits. The sample mean of six treatment populations in each of the cultivar exhibited highly superior quantitative phenotypic traits compared to their parent cultivars. The higher values of heritability and genetic advance with a high genotypic coefficient of variation for most of the yield attributing traits confirmed the possibilities of lentil yield improvement through phenotypic selection. The number of pods and seeds per plant appeared to be priority traits in selection for higher yield due to their strong direct association with yield. The cluster analysis divided the total populations into three divergent groups in each lentil cultivar with parent genotypes in an independent group showing the high efficacy of the mutagens. Considering the highest contribution of yield trait to the genetic divergence among the clustered population, it was confirmed that the mutagenic treatments created a wide heritable variation for the trait in the mutant populations. The selection of high yielding mutants from the mutant populations of DPL 62 (100 Gy) and Pant L 406 (100Gy + 0.1% HZ) in the subsequent generation is expected to give elite lentil cultivars. Also, hybridization between members of the divergent group would produce diverse segregants for crop improvement. Apart from this, the induced mutations at loci controlling economically important traits in the selected high yielding mutants have successfully contributed in diversifying the accessible lentil genetic base and will definitely be of immense value to the future lentil breeding programmes in India.

Morphological Characteristics and Productivity of Guinea Grass (Panicum maximum CV Purple Guinea) Irradiated with Gamma-Ray

“…High heritability and GVC values were obtained only in the characters of shoot and tillers weights. The value of heritability due to gamma irradiation was also reported in other studies such as Vigna ,mungo (Sri Devi, 2012), lentils (Laskar & Khan, 2017), sorghum (Anand & Kajjidoni, 2014), chick-pea (Amri-Tiliouine et al, 2018), mungbean (Vigna radiata) (Dhole & Reddy, 2018), pinger millet (Umeshkumar et al, 2015), and rice (Islam et al, 2015).…”

High Quantitative Trait Variability in Faba Bean Mutagenized Population for High-yielding Breeding Program in Saudi Arabia

“…Some traits had lower mean values than the control plants, while the others did not differ. Similar results in variation by comparing mean values for some quantitative traits between mutant populations and control plants in the M2 and M3 generation of lentils (Laskar and Khan 2017), in Mungbam (Dewanjee and Sarkar 2018), in cowpea (Nair and Mehta 2014;Bind and Dwivedi 2014) and in grasspea (Singh and Sadhukhan 2019) induced by gamma radiation.…”

Phenotypic diversity in mutagenized population of urdbean (Vigna mungo (L.) Hepper)

“…Genetic architecture of grain yield can be better resolved through component traits than yield per se , as yield is the end product of multiple interactions between various component traits ( Laskar and Khan, 2017 ). In the present investigation, the observations on different quantitative traits revealed a wide extent of genetic variability induced through individual and combination treatments of gamma rays and EMS.…”

“…The FAO/IAEA Mutant Variety Database ( https://mvd.iaea.org accessed on 24 January, 2021) records show that out of total 466 released mutant varieties of legumes, only 9 have been released in urdbean till date, indicating that the crop is less exploited for mutation breeding. Therefore, sustained efforts are needed to develop reproducible mutation protocols for creating novel genes controlling economically important traits, especially in India where the scope for agricultural intensification is very high ( Laskar and Khan, 2017 ).…”