Seeds of two varieties (PUSA-212 and BG-256) of chickpea (Cicer arietinum L.) were treated with 0.03% concentration of hydrazine hydrate (HZ), methylmethane sulphonate (MMS) and sodium azide (SA) for 6 h. The M 2 generation was grown from single plant M 1 progeny seeds. Five morphological mutants (dwarf, bushy, one sided branch, narrow leaf and gigas) were isolated in M 2 generation. Some of these mutants may be directly used in selection whereas some are useful in combination breeding programs.
The present study was conducted to assess the extent of induced genetic variability and to determine the inheritance pattern of various yield contributing phenotypic traits in M
2
and M
3
generations of urdbean following mutagenesis with single and combination treatments of gamma rays and ethyl methanesulphonate (EMS). The mean number of seeds per pod and 100-seed weight increased in all the mutagenic treatments in both the varieties with few exceptions in M
2
generation. Mean pod length although increased considerably, however it did not differ significantly in most of the mutagenic treatments. In M
3
generation, 0.2% EMS and 300 Gy γ rays+0.2% EMS treatments induced maximum increase in mean pod length, seeds per pod and 100-seed weight in both the varieties. Genetic parameters showed manifold increase in most of the mutagenic treatments and varied from trait to trait. Increased genetic variability for economically important traits in the selected mutant lines has successfully contributed in diversifying the accessible genetic base which could be exploited for subsequent improvement of urdbean through phenotypic selection.
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