Assessment of the role of meta-topolins on in vitro produced phenolics and acclimatization competence of micropropagated ‘Williams’ banana

Aremu, Adeyemi O.; Bairu, Michael W.; Szüčová, Lucie; Doležal, Karel; Finnie, J.F.; Staden, J. Van

doi:10.1007/s11738-012-1027-6

Cited by 71 publications

(26 citation statements)

References 40 publications

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“…Our findings also suggest that mT raised plantlets were more easily acclimatized than BA raised regenerants. A similar positive effect of mT has been well documented in earlier reports (Bairu et al 2007, Valero-Aracama et al 2010, Aremu et al 2012. Our results also show that mT raised regenerants had a higher number of healthy roots compared to BA raised regenerants, therefore, they were more resistant to environmental stresses in field conditions.…”

Section: Resultssupporting

confidence: 91%

“…Micropropagation of this recalcitrant species has been reported earlier through juvenile (Yadav et al 1990, Jain and Babbar 2000, Rathore et al 2004 and mature tissues (Naaz et al 2014). Continuous research in plant tissue culture  tries to reduce physiological disorders during in vitro growth and during an acclimatization phase (Aremu et al 2012). However, no reports are available on comparative analysis of a novel aromatic cytokinin meta-topolin (mT) and benzyladenine (BA) on protection of juvenile plantlets against stresses induced by ex-vitro transfer.…”

Section: Introductionmentioning

confidence: 99%

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Meta-topolin improved micropropagation in Syzygium cumini and acclimatization to ex vitro conditions

Naaz¹,

Hussain²,

Anis³

et al. 2019

Biologia plant.

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An efficient micropropagation system was developed for a recalcitrant woody tree Syzygium cumini (L.) Skeels using nodal explants excised from 15-d-old aseptic seedlings. The explants were employed on an Murashige and Skoog (MS) medium supplemented with different concentrations (1.0-10.0 μM) of cytokinins, such as benzyladenine (BA), kinetin (Kin), meta-topolin (mT), or 2-isopentyl adenine (2ip), either alone or in combination with different concentrations (1.0-3.0 μM) of auxins, such as indole-3-acetic acid (IAA), indole-3-butyric acid (IBA), or α-naphthalene acetic acid (NAA). Of the cytokinins tested, mT proved to be best for shoot bud induction and proliferation. Among the tested combinations, a maximum regeneration (90 %) with a mean shoot number of 25.33 ± 0.33 and a shoot length of 5.20 ± 0.11 cm were recorded on the MS medium containing 5.0 μM mT + 2.0 μM NAA after 12 weeks of incubation. Further 4-week incubation on optimum 5.0 μM mT before transfer to a secondary medium consisting of MS + 5.0 μM BA + 2.0 μM NAA yielded up to 51 microshoots with an average length (6.53 cm). For in vitro rooting, healthy shoots (about 5 cm) were excised and incubated on the half or full strength MS medium enriched with different concentrations (1-7.5 µM) of NAA. A substantial increase in rhizogenic competency (15 %) was observed in shoots raised on a medium with mT with a mean root number of 6.33 ± 0.10 and a mean length of 5.13 ± 0.21 cm on the half MS supplemented with 5.0 µM NAA after 4 weeks. A maximum of 95 % plantlets regenerated on the medium with mT was successfully acclimatized and established in earthen pots under field conditions. The consistent increases in activities of superoxide dismutase, catalase, glutathione reductase, and ascorbate peroxidase during acclimatization indicate that mT raised plantlets response well to the stress induced by ex vitro conditions.

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Section: Resultssupporting

confidence: 91%

Section: Introductionmentioning

confidence: 99%

Meta-topolin improved micropropagation in Syzygium cumini and acclimatization to ex vitro conditions

Naaz¹,

Hussain²,

Anis³

et al. 2019

Biologia plant.

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“…However, m-T clearly stimulated the biosynthesis of verbascoside and other phenylethanoids to a greater degree than other cytokinins: 1 mg/L of m-T increased verbascoside to a 3-fold higher level compared to controls. Although no study has described the use of topolins for bioactive compound production in the micropropagated Salvia shoots, m-T has been found to increase total phenolic, flavonoid and proanthocyanidin content in the aerial parts of regenerated banana plantlets [34]. Amoo et al [35] also report significantly higher levels of total phenolics, flavonoids and tannins in regenerated shoots of Aloe arborescens obtained on medium supplemented with m-T than BAP; however, the beneficial effects of meta-topoline are not apparent for all secondary metabolites, as the shoots regenerated with BAP produced higher iridoid levels than those with m-T.…”

Section: Discussionmentioning

confidence: 99%

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

et al. 2020

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The present study demonstrates hormonal control of Salvia viridis growth and development using four different purine-type cytokinins at different concentrations. The addition of cytokinins significantly increased biomass of cultures, proliferation rate, and, interestingly, secondary metabolite production. The best response in terms of multiplication ratio was recorded on Murashige and Skoog medium supplemented with 0.5 mg/L BPA (N-benzylotetrahydropyranyl adenine), while the greatest biomass accumulation was achieved when supplemented with 1 mg/L m-T (meta-topoline). Quantitative UPLC-DAD analysis of the hydromethanolic extract from S. viridis culture revealed the presence of 12 polyphenols: seven phenolic acids and five phenylethanoids. The highest total content of polyphenolic compounds was found in shoots cultivated on medium with 2 mg/L BPA (18.66 mg/g DW): almost twice that of control shoots. The medium was also the most optimal for the biosynthesis of rosmarinic acid, the predominant phenolic acid. However, the greater phenylethanoid accumulation was stimulated by 1 mg/L m-T: the metabolite content was above three times higher than that found in shoots grown on the control medium (8.03 mg/g DW vs. 2.37 mg/g DW). Hence, it was demonstrated that phytohormones are capable of influencing not only vital physiological processes, but therapeutic potential of plants as well. Therefore, the cytokinin-based sage cultures may be also considered as the alternative sources of bioactive compounds.

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“…Phenolic and condensed tannin contents were evaluated as detailed by Makkar et al (2007) while flavonoid was determined based on the protocols outlined by Aremu et al (2012). The methods of Levieille and Wilson (2002) were used for the determination of iridoid content.…”

Section: Methodsmentioning

confidence: 99%

Changes in phytochemical content and pharmacological activities of three Chlorella strains grown in different nitrogen conditions

et al. 2015

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The phytochemical content and biological activity of three Chlorella strains cultured in low (35 mg L −1 ) or high (700 mg L −1 ) nitrogen (N) and harvested on days 5 and 10 were evaluated. High N resulted in a higher biomass in Chlorella MACC 438 and MACC 452 while MACC 555 produced a higher biomass in low N. MACC 555 (low N/ day 5) had the highest phenolic content, and MACC 438 in low N/day 5 and high N/day 5 accumulated the highest flavonoids and condensed tannins, respectively. Iridoids were most abundant in MACC 452 on low N/day 10. Harvest time had the greatest effect on the phytochemical content with phenolics, flavonoids, and condensed tannins decreasing over time and iridoids increasing in low N and decreasing in high N conditions. Extracts were more active in β-carotene-linoleic acid model compared to 2,2-diphenyl-1-picrylhydrazyl (DPPH) free radical scavenging assay. Most extracts had good antimicrobial activity. Extracts became less potent over time in the antioxidant, acetylcholinesterase inhibitory (AChE), and antimicrobial assays when growing in low N and more potent in the antioxidant and AChE assays when grown in high N. Thus, phytochemical content and biological activities of the three Chlorella strains were affected by N levels, harvest time, and strain.

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Assessment of the role of meta-topolins on in vitro produced phenolics and acclimatization competence of micropropagated ‘Williams’ banana

Cited by 71 publications

References 40 publications

Meta-topolin improved micropropagation in Syzygium cumini and acclimatization to ex vitro conditions

Meta-topolin improved micropropagation in Syzygium cumini and acclimatization to ex vitro conditions

The Stimulatory Effect of Purine-Type Cytokinins on Proliferation and Polyphenolic Compound Accumulation in Shoot Culture of Salvia viridis

Changes in phytochemical content and pharmacological activities of three Chlorella strains grown in different nitrogen conditions

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