Assessment of the hypoglycemic effect of Bixin in alloxan-induced diabetic rats: <i>in vivo</i> and <i>in silico</i> studies

Keita, Hady; Santos, Cleydson Breno Rodrigues dos; Ramos, Matheus Mercês; Padilha, Elias Carvalho; Serafim, Rodolfo Bortolozo; Castro, Andrés Navarrete; Amado, Jesús Rafael Rodríguez; Silva, Gabriel Monteiro da; Ferreira, Irlon M.; Giuliatti, Silvana; Carvalho, José Carlos Tavares

doi:10.1080/07391102.2020.1724567

Cited by 8 publications

(6 citation statements)

References 81 publications

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“…Finally, these results are in agreement with the studies conducted respectively on the seed cotyledon and leaves of Chrysophyllum albidum at doses between 250 and 1000 mg/kg bw [48,49] on the one hand, and on the other hand on Bixin (10 mg/kg bw) extracted from the seeds of Bixa orellana [50]. These authors have shown that these extracts significantly decrease the glucose level in normal and diabetic rats induced by alloxane.…”

Section: Discussionsupporting

confidence: 90%

"Evaluation of an Active Butanol Fraction Effects of Blighia Unijugata (Sapindaceae) Leaves on Some Biochemical Blood Parameters in Male Wistar Rats"

Frédéric¹

2021

BJSTR

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Background and Objective: Blighia unijugata is usually used for its many therapeutic indications in Côte d'Ivoire. This study evaluated the effects of oral administration of an active butanol fraction of Blighia unijugata for 28 days on some biochemical blood parameters of male rats. Materials and Methods:The active butanolic fraction of Blighia unijugata (BFBu) was prepared by successive liquid-liquid extractions of the total ethanolic extract of this plant with water, hexane, chloroform, ethyl acetate and n-butanol. Male albino rats (Rattus norvegicus) of Wistar strain were assigned into four groups of six animals each including a control group receiving distilled water and three test groups receiving BFBu at 50; 500 and 1000 mg/kg bw for 28 days respectively. The substances were administered orally, once a day. Blood samples were taken on days 7; 14; 21 and 28 of treatment by puncture at the level of the orbital sinus of the eye of rats previously anesthetized with ether for the determination of biochemical parameters using an automatic analyzer (KENZA MAX Biochemis Try, France). Biochemical analysis included creatinine, urea, total protein, transaminases (AST, ALT), total cholesterol, HDL-cholesterol, triglycerides, bilirubins (direct and indirect), glucose, electrolytes (sodium, potassium, chlorine, calcium, magnesium) and atherogenicity index.Results: At repeated doses of 50, 500 and 1000 mg/kg bw for 28 days, the extract contributed to a significant increase in certain biochemical parameters such as AST, ALT, urea, creatinine, and total proteins. On the other hand, this same extract evaluated under the previous conditions, caused a decrease in the levels of glucose, total cholesterol, HDL-cholesterol, LDL cholesterol, bilirubins (direct and indirect) and had no noticeable effect on the variation in the concentration of certain electrolytes. Conclusion:At high doses, the active butanol fraction of Blighia unijugata caused mid-term hepatic and renal toxicity. However, at a dose of 50 mg/kg bw, this fraction is non-toxic. This fraction has hypoglycemic effects and does not predispose rats to the risk of atherosclerosis and coronary heart disease.

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Section: Discussionsupporting

confidence: 90%

"Evaluation of an Active Butanol Fraction Effects of Blighia Unijugata (Sapindaceae) Leaves on Some Biochemical Blood Parameters in Male Wistar Rats"

Frédéric¹

2021

BJSTR

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“…Several studies have demonstrated the beneficial effects of medicinal plants in reducing blood glucose level using different models of diabetes induction in animals [2,23,25]. In the present study, we chose alloxan monohydrate to induce diabetes as it has been widely used in the recent years to develop stable hyperglycemia in experimental animal model of diabetes [18,19].…”

Section: Discussionmentioning

confidence: 99%

“…Diabetic group exhibited a continuous decrease in body weight over a period of 21 days. The decrease of body weight could be due to insulin deficiency or abnormalities in the catabolism of macronutrients such as fat and protein leading to extreme tissue protein loss and muscle wastage [23,48]. The oral administration of H. sabdariffa extract and Glibenclamide in alloxan-induced diabetic rats for 3 weeks increased the body weight, which indicates a better control of high blood glucose in these rats.…”

Section: Discussionmentioning

confidence: 99%

“…Diabetes was induced by a single intraperitoneal injection of freshly prepared alloxan (150 mg/kg) dissolved in 0.9% saline after overnight fasting for 12 h except the control group. Then, rats were allowed to free access of 5% glucose solution for 24 hours to prevent hypoglycemia [23]. After 3 days, the fasting blood glucose levels in the rats were assessed to validate the successful induction of diabetes mellitus.…”

Section: Diabetes Inductionmentioning

confidence: 99%

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Antidiabetic potential of Hibiscus sabdariffa extract in alloxan-induced diabetic rats

Hamadjida¹,

Metechie²,

Tchiengang³

et al. 2023

GSC Biol. Pharm. Sci.

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Plants are known to possess relatively high efficacy in the treatment of several chronic diseases with fewer adverse effects. In the recent years, numerous medicinal plants have been reported to be effective in treating diabetes. Hence, the present study aims to evaluate the antidiabetic property of hydroalcoholic extract of Hibiscus sabdariffa (HS) calyces in diabetic rats. Diabetes was induced by single intraperitoneal injection of alloxan (150 mg/kg, b.w), in male Wistar rats. Diabetic rats were administered daily oral doses of HS (100, 200 and 400 mg/kg body weight) and Glibenclamide (10 mg/kg) for 21 days. Then, blood glucose levels, oral glucose tolerance test and lipid profiles were determined. Treatment with HS resulted in a significant dose dependent reduction of blood glucose levels accompanied by a significant improvement in body weight. The extract also enhanced the glucose tolerance and significantly decreased cholesterol, triglycerides, and low-density lipoproteins levels while the high-density lipoproteins level significantly increased. From the results obtained, it can therefore be concluded that Hibiscus sabdariffa has an antidiabetic effect in alloxan-induced diabetic rats.

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“…Bixin also shows in silico selectivity on peroxisome proliferator-activated receptors (PPARs), particularly by the peroxisome proliferatoractivated receptor gamma (PPARγ), which supports the hypoglycemic activity of Bixin. 22…”

Section: Hypoglycemic Activitymentioning

confidence: 99%

Isolation and biological activities of naturally occurring apocarotenoid bixin

Patra¹,

Kar²,

Bag³

2021

Prayogik Rasayan

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The naturally occurring apo-carotenoid bixin is obtained from annatto (Bixa orellana). It is unique among the natural carotenoids through its water solubility and easily extractability from the seed coat of Bixa orellana. Bixin is (2E,4E,6E,8E,10E,12E,14E,16Z, 18E)-20- methoxy-4,8,13,17-tetramethyl-20-oxicosa 2,4,6,8,10,12,14,16,18-nonaenoic acid, an apo-carotenoid which is a terpenoid compound derived from the oxidative cleavage of carotenoids. Several biological activities of bixin has been reported earlier such as selective antimyeloma effects of cis-bixin, antinociceptive and anti-Inflammatory effects, antioxidant action against cis-platine induced chromosome in rats, hypoglycemic effect in alloxan-induced diabetic rats, allodynia, anxious and depressive-like behaviors, targeting NRF2 for improved skin barrier function and photoprotection, antiatherogenic effect in hypercholesterolemic rabbits, triggering apoptosis of cancer cells, immunomodulatory role in dogs, sensitization of human melanoma cells to dacarbazine-induced apoptosis, sensitization of human lung cancer and cervical cancer cell to cisplatin in vitro, anti-SARS-CoV-2 activity, antibacterial activity of cis-bixin conjugated silver nanoparticles, protective effect on carbon tetrachloride-induced hepatotoxicity in rats, bixin/crocetin nanoparticles on quality and shelf life improvement of cheddar cheese etc. In this review, the biological properties of bixin has been discussed. A practical method for the isolation of bixin 1 from the seed coat of Bixa orellana commonly known as “Sinduri” (in Bengali) and its characterization has also been reported.

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Assessment of the hypoglycemic effect of Bixin in alloxan-induced diabetic rats: in vivo and in silico studies

Cited by 8 publications

References 81 publications

"Evaluation of an Active Butanol Fraction Effects of Blighia Unijugata (Sapindaceae) Leaves on Some Biochemical Blood Parameters in Male Wistar Rats"

"Evaluation of an Active Butanol Fraction Effects of Blighia Unijugata (Sapindaceae) Leaves on Some Biochemical Blood Parameters in Male Wistar Rats"

Antidiabetic potential of Hibiscus sabdariffa extract in alloxan-induced diabetic rats

Isolation and biological activities of naturally occurring apocarotenoid bixin

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