Assessment of the genotoxicity of propineb in mice bone marrow cells using micronucleus assay

Assessment of the Combined Effects of Acetamiprid and Propineb in Vivo

“…1.25 µg mL -1 concentration of Acetamiprid significantly decreased PCE/NCE ratio for 24h compared with negative control, but not in others. In our earlier publication (Rasgele et al, 2014), significant reduction for the PCE/NCE ratio was observed at 25 and 50 µg mL -1 concentrations of Propineb for 24 h and at the highest (50 µg mL -1 ) concentration for 48 h. Moreover, the mixture of Acetamiprid and Propineb showed a significant synergistic effect at the all concentrations and treatment times due to reduction of PCE/NCE ratio compared to negative control (Table 1).…”

“…Pınar GÖÇ RASGELE Propineb individually showed genotoxic effect in mice bone marrow cells (Rasgele 2014); but, Acetamiprid did not show any genotoxic affect. It can be found similar results in earlier literature (Rasgele et al, 2014).…”

“…The cells were removed from bone marrow with fetal calf serum, and the homogenate was centrifuged for 10 min at 2000 rpm. The pellets were resuspended in a drop of serum, plastered it on a slide glass, fixed with methanol and stained with May Grunwald for 3 min, May Grunwald:distilled water (1:1) for 2 min, 10 % Giemsa in Sorensen buffer for 10 min (Rasgele et al, 2014).…”

“…It was observed that many pesticides in which propineb is implicit cause a significant increase in CA and MN frequencies of many people who use pesticides in agricultural areas (Bolognesi et al, 1993;Pasquini et al, 1996;Falck et al, 1999;Pastor et al, 2001;Pazy-Mino et al, 2002). In our earlier publication (Rasgele et al, 2014), it was showed in mice that Propineb induced significantly formation of micronucleus at 25 and 50 µg mL -1 concentrations for 24 h and at the highest (50 µg mL -1 ) concentration for 48 h. Moreover, significant decline for PCE/NCE ratio was obtained at the same concentrations for 24 and 48 h. Numerous genotoxicity markers such as gene mutation assay, chromosome aberration assay and DNA damage assay have been developed for the detection of early biological effects induced by pesticides (Sato and Tomita, 2001). Micronucleus (MN) assay is a tool of great interest in toxicity risk assessment due to its simplicity, accuracy, wide tissue applicability and has been recently used for identification of genotoxic effects (Heddle, 1973;Schmid, 1975;Decordier and Kirsch-Volders, 2006).…”

“…The aim of this study was to investigate the frequencies of micronucleated erythrocytes following exposure mixtures of Acetamiprid and Propineb in bone marrows cells of mice using micronucleus assay due to commonly use of these pesticides and lack of information about their genotoxicities in vivo. Although we have published our results related to effect of Propineb (Rasgele et al, 2014), the data from that publication will be used to be able to make clear explanations and discussions in this paper because pesticide mixture we used has Propineb. …”