Assessment of the effect of growth temperature on the lipid composition of <i>Serratia marcescens</i> using laser desorption mass spectrometry

Hara, Hisako; Cotter, Robert J.; Cooks, R. Graham

doi:10.1002/rcm.1290010702

Cited by 6 publications

(5 citation statements)

References 5 publications

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“…Ornithine amide lipids with the general structure I are very widely distributed in bacteria (Wilkinson, 1988; Lanéelle et al, 1990) . However, the production of such lipids can depend critically on the cultural conditions used (Wilkinson, 1972; Minnikin & Abdolrahimzadeh, 1974; Wilkinson & Caudwell, 1980; Moule & Wilkinson, 1987; Hara & Cotter, 1987). Thus, further work would be necessary to determine whether the absence of ornithine amide lipids from P .…”

Section: Discussionmentioning

confidence: 99%

Polar lipids and fatty acids of Pseudomonas caryophylli, Pseudomonas gladioli and Pseudomonas pickettii

Galbraith

Wilkinson

1991

Microbiology

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The polar lipids and their fatty acid components in Pseudomonas cmyophyffi, Pseudomonas g f d o f i and Pseudomonas pickeftii have been identified. In addition to diphosphatidylglycerol and phosphatidylglycerol (a trace only for P. picketfir?, all three species contained two forms of phosphatidylethanolamine differing in the presence or absence of a-hydroxy fatty acids. This seems to be a distinctive feature of species in Pseudomonas RNA homology group 11. Also, P. caryophyffi and P. g f d o f i (but not P. pickeftir? produced two forms of ornithine amide lipid, differing in the nature (hydroxy or non-hydroxy) of the ester-linked fatty acid. In all three species, the major non-hydroxy acids were hexadecanoic acid, a hexadecenoic acid, an octadecenoic acid, and cyclopropane derivatives of the monoenoic acids. The a-hydroxy acids were the derivatives of the same components, while the amide-linked acid of the ornithine amide lipids was mainly or entirely 3-hydroxyhexadecanoic acid. The possible taxonomic implications of the data are discussed.

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Section: Discussionmentioning

confidence: 99%

Polar lipids and fatty acids of Pseudomonas caryophylli, Pseudomonas gladioli and Pseudomonas pickettii

Galbraith

Wilkinson

1991

Microbiology

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“…Since the lipid portion (lipid A) of endotoxic lipopolysaccharide (LPS) has been shown to be responsible for most of the biological activities of these macromolecules, it is the focal point of such studies. However, for both biological and structural studies, two complications arise: the lipid moiety is often heterogeneous (1,20), and its composition can vary with bacterial growth conditions (11,13,14,18,25,27,30,33).…”

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confidence: 99%

“…Few of these variants have been tested biologically, but some, especially those touching the phosphate groups (24,28,29), may well have an effect on the biological activities of lipid A. Several reports have already testified to the influence of bacterial growth temperature and medium on the LPS composition of endotoxins (11,13,14,18,25,27,30,33), and the observed variation may be attributed to differences in culture conditions. Thus, the analytical results obtained with one preparation should not be assumed to apply to all preparations obtained from a given strain.…”

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confidence: 99%

Comparison of lipids A of several Salmonella and Escherichia strains by 252Cf plasma desorption mass spectrometry

1993

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Plasma desorption mass spectrometry has recently been used with success to characterize underivatized lipid A preparations: the major molecular species present give signals indicating their masses, from which probable compositions could be inferred by using the overall composition determined by chemical analyses. In the present study, plasma desorption mass spectrometry was used to compare structures in lipid A preparations isolated from several smooth and rough strains of Escherichia and Salmonella species. Preparations isolated from strains of both genera revealed considerable variation in degree of heterogeneity (number of fatty acids and presence or absence of hexadecanoic acid, phosphorylethanolamine, and aminoarabinose). Molecular species usually associated with Salmonella lipid A were found in preparations from Escherichia sp. In addition, preparations from three different batches of lipid A from one strain of Salmonella Minnesota showed significant differences in composition. These results demonstrate that preparations used for biological and structural analyses should be defined in terms of their particular molecular constituents and that no generalizations based on analysis of a single preparation should be made.

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“…Lipoamino acids were isolated from the total cellular lipids of S. marcescens (NS 38 Purification was confirmed as a single spot on the TLC plate. The structures of lipoamino acids (serratamolide and ornithine lipid) were identified by gas chromatography/mass spectrometry (GC/MS) and fast atom bombardment mass spectrometry (FAB/MS), as reported previously [12,13] (Fig. 1).…”

Section: Preparation Of Lipoamino Acidsmentioning

confidence: 90%

“…However, the role of bacterial membrane lipid on phagocytosis has not been reported. Lipoamino acid is a cell membrane component of some bacterial species such as Pseudomonas, Brucella, Bordetella and Serratia [1][2][3][4][5][6][7][8][9][10][11][12][13].…”

Section: Introductionmentioning

confidence: 99%

Stimulation and inhibition of polymorphonuclear leukocytes phagocytosis by lipoamino acids isolated from Serratia marcescens

Miyazaki

1993

FEMS Immunology and Medical Microbiology

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We reconfirmed that the LD50s of hemolytic Enterococcus faecalis strains were significantly less than those of nonhemolytic E. faecalis strains in normal mice. Hemolysin produced by E. faecalis lysed human, horse, rabbit, and mouse erythrocytes, but not cow and sheep erythrocytes. Sphingomyelin comprises a part of the lipid composition of the erythrocyte membrane of all mammalian species tested. But phosphatidylcholine exists only in human, horse, rabbit, and mouse. These two lipids inhibited lysis of horse erythrocytes by hemolytic E. faecalis. Phosphatidylcholine is probably the binding component on the membrane of erythrocytes for E. faecalis hemolysin. The hemolytic culture supernatant lysed not only erythrocytes but also mouse polymorphonuclear neutrophils (PMNs) and macrophages.

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Assessment of the effect of growth temperature on the lipid composition of Serratia marcescens using laser desorption mass spectrometry

Cited by 6 publications

References 5 publications

Polar lipids and fatty acids of Pseudomonas caryophylli, Pseudomonas gladioli and Pseudomonas pickettii

Polar lipids and fatty acids of Pseudomonas caryophylli, Pseudomonas gladioli and Pseudomonas pickettii

Comparison of lipids A of several Salmonella and Escherichia strains by 252Cf plasma desorption mass spectrometry

Stimulation and inhibition of polymorphonuclear leukocytes phagocytosis by lipoamino acids isolated from Serratia marcescens

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