Polar lipids and fatty acids of Pseudomonas caryophylli, Pseudomonas gladioli and Pseudomonas pickettii

Abstract

The polar lipids and their fatty acid components in Pseudomonas cmyophyffi, Pseudomonas g f d o f i and Pseudomonas pickeftii have been identified. In addition to diphosphatidylglycerol and phosphatidylglycerol (a trace only for P. picketfir?, all three species contained two forms of phosphatidylethanolamine differing in the presence or absence of a-hydroxy fatty acids. This seems to be a distinctive feature of species in Pseudomonas RNA homology group 11. Also, P. caryophyffi and P. g f d o f i (but not P. pickeftir? produced two forms of ornithine amide lipid, differing in the nature (hydroxy or non-hydroxy) of the ester-linked fatty acid. In all three species, the major non-hydroxy acids were hexadecanoic acid, a hexadecenoic acid, an octadecenoic acid, and cyclopropane derivatives of the monoenoic acids. The a-hydroxy acids were the derivatives of the same components, while the amide-linked acid of the ornithine amide lipids was mainly or entirely 3-hydroxyhexadecanoic acid. The possible taxonomic implications of the data are discussed.

Phenotypic variation of lipid composition in Burkholderia cepacia: a response to increased growth temperature is a greater content of 2-hydroxy acids in phosphatidylethanolamine and ornithine amide lipid

“…However, the phosphorus content of the lipids was relatively constant and indi- Galbraith & Wilkinson, 1991), broad signals on a noisy background were obtained by the NMR method, so that separate PE peaks were not observed and there was partial overlap of those for DPG and PG. Chromatographic resolution on TLC was also incomplete, so the data obtained by both methods (Table 2) are only given for lipid pairs.…”

“…(Galbraith & Wilkinson, 1991;Yabuuchi et al, 1992;Phung et al, 199Sa), whereas certain species recently excised from Burkholderia and Alcaligenes to form the new genus Ralstonia lack both forms of OL (Galbraith & Wilkinson, 1991 ;Yabuuchi et al, 1995). Phenotypic variation of lipid composition in bacteria, including the stimulation of OL production by growth under phosphorus-limiting conditions for at least some Gramnegative species, is a well-known phenomenon (Wilkinson, 1988).…”

“…One anomaly (Yabuuchi et al, 1995) is posed by Burkholderia andropogonis, which contains both forms of PE but neither form of OL, thus resembling Ralstonia spp. in this respect (Galbraith & Wilkinson, 1991 ;Yabuuchi et al, 1992Yabuuchi et al, , 1995 , 1992). Lipids of the general structure 1 with only 2-hydroxy fatty acids in ester linkage (like OL2) are present in Tbiobacillus t h ooxidans (Knoche & Shively, 1972) and Gluconobacter cerinus (Tahara et al, 1976).…”

“…(Galbraith & Wilkinson, 1991;Yabuuchi et al, 1992). One anomaly (Yabuuchi et al, 1995) is posed by Burkholderia andropogonis, which contains both forms of PE but neither form of OL, thus resembling Ralstonia spp.…”

Transfer of Pseudomonas plantarii and Pseudomonas glumae to Burkholderia as Burkholderia spp. and Description of Burkholderia vandii sp. nov.

“…These bacteria have the same major cellular fatty acid and ubiquinone system components. Galbraith and Wilkinson (14) reported that the nonhydroxy fatty acids of B. caryophylli and B. gladioli consisted of large amounts of n-C16:", n-C18:1, and cyclopropane C,7:o acids and that B. pickettii contained large amounts of n-Clh:O, n-c16:1, n-C18:,, and cyclopropane CI7:" acids. The differences in our results may have been due to the culture age of the cells used, because a decrease in the amount of h?-c16:1 acid and an increase in the amount of cyclopropane C17:o acid with age have been reported previously (48).…”