Assessment of the degree of restriction fragment length polymorphism in Brassica

Figdore, Scott S.; Kennard, W. C.; Song, Keming; Slocum, Mary K.; Osborn, Thomas C.

doi:10.1007/bf00258042

Cited by 108 publications

(19 citation statements)

References 10 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…Generally out- crossing species such as maize (Smith, 1988), Brassica (Figdore et al ., 1988) and potato (Gebhardt et al ., 1989) show a high level of genetic diversity among cultivated types . For instance within Brassica rapa, 61 .8% fragments were phylogenetically informative .…”

Section: Discussionmentioning

confidence: 99%

Nuclear RFLP between pepper cultivars (Capsicum annuum L.)

1993

View full text Add to dashboard Cite

Forty one nuclear probes, distributed across the different linkage groups of the previously published map, were used to examine restriction fragment length polymorphism between cultivated peppers . Total DNA from thirteen accessions of Capsicum annuum var . annuum and one accession of C. baccatum var. pendulum was separately cut with ten restriction enzymes . The analyses were restricted to only one enzyme per probe to reduce the polymorphism redundancy. Nei & Li's genetic distances between accessions were calculated from the 141 resultant nuclear DNA restriction fragments . The genetic variation was larger between C . annuum and C. baccatum than between C. annuum cultivars . Large fruited related accessions closely clustered together .Distances between the small fruited cultivars were larger than within the bell pepper group . A correspondence analysis performed on differences between the global RFLP patterns of each accessions of C. annuum revealed the particular genomic structure of four small fruited cultivars : Criollo de Morelos 334, H3, Perennial and Doux Long des Landes . The percentage of probes revealing at least one RFLP with at least one enzyme ranged from zero to fifty percent for all the pairwise comparisons of C . annuum accessions . 82 presumptive loci were detected with a mean number of 1 .46 alleles per locus within C . annuum and 1 .83 within all the accessions . This result indicates that molecular markers will be more usable in intraspecific study of C.annuum than isozyme markers .

show abstract

Section: Discussionmentioning

confidence: 99%

Nuclear RFLP between pepper cultivars (Capsicum annuum L.)

1993

View full text Add to dashboard Cite

show abstract

“…Among others, out-crossing species such as maize (Smith 1988), Brassica (Figdore et al 1988) and potato (Gebhardt et al 1989) show a high level of genetic diversity. Conversely, autogamous species like soybean (Apuya et al 1988), tomato (Miller and Tanksley 1989) and wheat (Chao et al 1989) show relatively low levels of polymorphism between cultivars.…”

Section: Genetic Diversitymentioning

confidence: 99%

Genetic Variability in Pepper (Capsicum annuum L.) Estimated by Morphological Data and Amplified Fragment Length Polymorphism Markers

2005

View full text Add to dashboard Cite

Data on genetic similarity among crop cultivars is of vital importance for the plant breeder. The objectives of this study were to group pepper (Capsicum annuum L.) genotypes into clusters according to their distances as estimated by morphological traits and amplified fragment length polymorphism (AFLP) markers and to assess the relationships between the two. Thirty-nine pepper genotypes obtained from different countries were grown in the greenhouse at University of the Free State, South Africa, during 2001 and 2002 in a randomized complete block design with three replications. A total of 20 different morphological traits were measured and six AFLP primer pairs were used to estimate pairwise genetic distances. Both datasets showed high genetic distances among the different genotypes, indicating high genetic diversity among them. The mean genetic distance among Ethiopian pungent elongated-fruit genotypes, was lower than that between them and the introduced ones. Morphological and AFLP distance estimations generally clustered together genotypes with similar fruit sizes. Significant, positive correlation was observed between morphological and AFLP diversity estimations. The narrow genetic basis among the Ethiopian pungent elongated-fruit cultivars suggests that the pepper breeding program of Ethiopia should focus on enriching its germplasm through local collection and introductions from other parts of the world.

show abstract

“…The level of polymorphism in Brassica crops is naturally high (Figdore et al 1988) making them attractive subjects for marker-assisted breeding. The construction of RFLP maps of B. napus (Ferreira et al 1994;Parkin et al 1995;Sharpe et al 1995;Uzunova et al 1995) has facilitated the marker-assisted selection of intervarietal substitution lines (Howell et al 1996) and the genetic mapping of genes affecting a range of agronomically important traits (Toroser et al 1995;Uzunova et al 1995;Fray et al 1997;Butruille et al 1999).…”

Section: Introductionmentioning

confidence: 99%

Mapping the mosaic of ancestral genotypes in a cultivar of oilseed rape (Brassica napus) selected via pedigree breeding

Sharpe¹,

Lydiate²

2003

Genome

View full text Add to dashboard Cite

Recent oilseed rape breeding has produced low glucosinolate cultivars that yield proteinaceous meal suitable for animal feed. The low glucosinolate character was introduced into modern cultivars from Brassica napus 'Bronowski', a cultivar that is agronomically inferior in most other respects. Residual segments of 'Bronowski' genotype in modern cultivars probably cause reduced yield, poorer winter hardiness, and lower oil content. The quantity and distribution of the 'Bronowski' genotype in the modern oilseed rape cultivar Brassica napus 'Tapidor' was investigated using a segregating population derived from a cross between 'Tapidor' and its high glucosinolate progenitor. This population was analyzed with 65 informative Brassica RFLP probes and a genetic linkage map, based on the segregation at 77 polymorphic loci, was constructed. The mapping identified 15 residual segments of donor genotype in 'Tapidor', which together occupy approximately 29% of the B. napus genome. Mapping the loci that control variation for the accumulation of total seed glucosinolates in the segregating population has identified three loci that together explain >90% of the variation for this character. All of these loci are in donor segments of the 'Tapidor' genome. This result shows the extent to which conventional breeding programmes have difficulty in eliminating residual segments of donor genotype from elite material.

show abstract

Assessment of the degree of restriction fragment length polymorphism in Brassica

Cited by 108 publications

References 10 publications

Nuclear RFLP between pepper cultivars (Capsicum annuum L.)

Nuclear RFLP between pepper cultivars (Capsicum annuum L.)

Genetic Variability in Pepper (Capsicum annuum L.) Estimated by Morphological Data and Amplified Fragment Length Polymorphism Markers

Mapping the mosaic of ancestral genotypes in a cultivar of oilseed rape (Brassica napus) selected via pedigree breeding

Contact Info

Product

Resources

About