Nuclear RFLP between pepper cultivars (Capsicum annuum L.)

Abstract

Forty one nuclear probes, distributed across the different linkage groups of the previously published map, were used to examine restriction fragment length polymorphism between cultivated peppers . Total DNA from thirteen accessions of Capsicum annuum var . annuum and one accession of C. baccatum var. pendulum was separately cut with ten restriction enzymes . The analyses were restricted to only one enzyme per probe to reduce the polymorphism redundancy. Nei & Li's genetic distances between accessions were calculated from the 141 resultant nuclear DNA restriction fragments . The genetic variation was larger between C . annuum and C. baccatum than between C. annuum cultivars . Large fruited related accessions closely clustered together .Distances between the small fruited cultivars were larger than within the bell pepper group . A correspondence analysis performed on differences between the global RFLP patterns of each accessions of C. annuum revealed the particular genomic structure of four small fruited cultivars : Criollo de Morelos 334, H3, Perennial and Doux Long des Landes . The percentage of probes revealing at least one RFLP with at least one enzyme ranged from zero to fifty percent for all the pairwise comparisons of C . annuum accessions . 82 presumptive loci were detected with a mean number of 1 .46 alleles per locus within C . annuum and 1 .83 within all the accessions . This result indicates that molecular markers will be more usable in intraspecific study of C.annuum than isozyme markers .

Genetic Variation in Bell Peppers (Capsicum annuum L.) Collected from Iraqi Local Markets by Using AFLP Marker

“…These results confirmed the fact that the least similarity has the largest genetic variation. The modern breeding of plant led to a significant decreases in the genetic variation of modern varieties [45], and this also explained by Paran et al [25] when they have a low percentage of the polymorphic varieties (13%) after using of 10 AFLP primers according to the dendrogram of this study; the large fruit of bell sweet pepper with low genetic variation separated compared to the variation of small fruit with high variation this is similar to the results [43], [46], [47] as well. …”

Diversidade genética entre acessos de pimentas e pimentões da Embrapa Clima Temperado

“…Outra possibilidade seria a de que os genótipos cultivados próximos (mesma ilha) sofreram cruzamentos e, com o passar das gerações, provocaram mistura entre as variedades. LEFEBVRE et al (1993) notaram que Capsicum annuum é altamente variável em comparação a culturas de autofecundação. Eles sustentam que a variação morfológica em C. annuum pode ser explicada pelo seu modo de reprodução e processo pelo qual a espécie passou até a sua domesticação.…”

Diversidad morfológica de poblaciones nativas de chile poblano

“…Meanwhile, González and Bosland (1991) indicate that the collection and characterization of local populations is necessary to obtain and find additional genetic resources that are not known and that the desirable characteristics can be transferred to commercial cultivars. Furthermore, Geleta et al (2005) suggest that reproductive behavior is determinant in the degree of genetic diversity among cultures; although Lefebvre et al (1993) mention that the reproductive behavior of C. annuum L. is very fickle, compared to other self-pollinating species, because most species of Capsicum, among them C. annuum L., are protogynous (Pickersgill, 1997;Djian-Caporalino et al, 2006) and there is a degree of "excursion" the stigma different for each genotype, which extends the possibility of crosspollination between populations (Pickersgill, 1997). The way of reproduction of these peppers, as well as the empirical selection that have made the producers of the region of the Sierra Nevada de Puebla has given the possibility of finding genetically diverse germplasm, mainly in characteristics of fruit, where you can find variability in weight , size and color.…”

“…Además, Geleta et al (2005) señalan que el comportamiento reproductivo es determinante en el grado de diversidad genética entre los cultivos; aunque Lefebvre et al (1993) mencionan que el comportamiento reproductivo de C. annuum L. es muy inconstante, en comparación con otras especies autógamas, debido a que la mayoría de las especies de Capsicum, dentro de ellas C. annuum L., son protogínicas (Pickersgill, 1997;Djian-Caporalino et al, 2006) y existe un grado de excersión del estigma diferente para cada genotipo, lo cual amplía la posibilidad de polinización cruzada entre las poblaciones (Pickersgill, 1997). La manera de reproducción de estos chiles, así como la selección empírica que han realizado los productores de la región de la Sierra Nevada de Puebla ha dado la posibilidad de encontrar germoplasma genéticamente diverso, principalmente en características de fruto, donde se puede encontrar variabilidad en peso, tamaño y coloración.…”