Assessment of survival of pathogenic bacteria in fresh vegetables through in vitro challenge test

Noor, Rashed; Malek, Mushfia; Rahman, Shohanur; Meghla, Monirunnessa; Acharjee, Mrityunjoy; Rahman, Mohammad Majibur

doi:10.1186/s40550-015-0021-3

Cited by 10 publications

(11 citation statements)

References 29 publications

(63 reference statements)

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“…Proliferation of microorganisms are very much likely (1) to edge the shelf life of vegetables; and (2) to spread and transmit diseases in humans and animals (15,39). Several of our research reported huge contaminating bacteria (including the drug-resistant ones) among a range of vegetables (carrot, lettuce, cucumber, tomato, chilli, onion, capsicum and coriander) collected from different sources (8,27,39).…”

Section: Microbiological Study Of Vegetables In Bangladeshmentioning

confidence: 83%

Transmission of pathogens within the commonly consumed vegetables: Bangladesh perspective

Feroz¹,

Noor²

2019

S.J. Microbiol.

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Fresh vegetables and fruits are prime source of fiber, vitamins and minerals in our daily diet. Nevertheless, raw vegetables act as a vector for transmitting pathogenic microbes and the majority of diseases are spread by the fecal-oral route at different point of harvesting, post harvesting, during transportation and storage conditions. Contaminated water, carrier materials, and unhygienic handlers are the crucial gateway for disease causing microbes in fresh vegetables. Recently Listeria spp., E. coli, and Salmonella spp. associated outbreak evident in mixed vegetables salad and other vegetable samples also showed pathogenic proliferation to some extent. Children are the major risk group and according to World Health Organization, their mortality rate is high due to food borne infections. In Bangladesh, the situation is more alarming in terms of pathogenic proliferation in raw vegetables. A lot of researches have been conducted in recent years on fresh vegetables and a wide range of vegetables are found to harbor pathogenic microorganisms inclusive of drug resistant bacteria which may affect the measures to combat harmful pathogens. Additionally, phytotoxic, natural contaminants and pesticides are also found to reside in raw vegetables which may be life threatening for both human and animal health as these particles magnify when we repeatedly consume them. The current review focus on the possible intervention of potential contaminants in the vegetable items and possible public health risks associated with their consumption along with a general guideline to combat contamination of fresh vegetables. Stamford Journal of Microbiology, Vol.8(1) 2018: 46-49

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Section: Microbiological Study Of Vegetables In Bangladeshmentioning

confidence: 83%

Transmission of pathogens within the commonly consumed vegetables: Bangladesh perspective

Feroz¹,

Noor²

2019

S.J. Microbiol.

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“…Many researches have been conducted in this aspect (28,(30)(31)(32)(34)(35)(36)(37). Our study continued such work with strawberries to determine antimicrobial activities.…”

Section: Resultsmentioning

confidence: 87%

“…Resistance of pathogenic bacteria is very critical phenomena in treating diseases caused by them (29,(34)(35)(36). This resistance can rapidly transfer to susceptible bacteria making the situation more worse.…”

Section: Resultsmentioning

confidence: 99%

Microbiological analysis for drug resistant pathogenic microorganisms with determination of the antibacterial properties found in Fragaria x ananassa (strawberry) samples

2017

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16Strawberry is a popular fruit item consumed by people all over the world for its exceptional flavor and aroma. This fruit is being used to prepare a wide range of food items. Strawberries when consumed by people without undergoing any processing, the condition may not be good enough. People may get sick after consuming raw strawberries. Our current study identified several pathogenic bacteria which are very known in causing health related problems. E. coli was found in one sample (1.0×10 2 cfu/g), Staphylococcus aureus was commonly present in all of the five samples (up to 1.2×10 6 cfu/g), Klebsiella spp. was found to be present in two samples (within 1.0×10 3 cfu/g) and Pseudomonas spp. was present in three samples (8.2×104 cfu/g), respectively. All isolated bacteria showed some degree of drug resistant traits against some commonly used antibiotics. The most important and promising part of the study was to successfully find the antimicrobial activity of strawberries against seven types of pathogenic bacteria.

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“…had been incapable of colony forming ability on the agar plates when exposed to oxidative stress which is often related to the raise of temperature 11,16 . The impacts of heat shock on bacterial species have been extensively studied revealing unfolded/ denatured proteins; the studies on the effect of cold shock apparently seem to be in scarce 2,6,7,11,15,17 . However, among the environmental stresses posed on bacterial population, the theme of cold shock is not that new too 15,18 .…”

Section: Introductionmentioning

confidence: 99%

In Vitro demonstration of Pseudomonas Growth and Phenotypic Examinations of the Cells Under Cold Shock

Moumita¹,

Tabassum²,

Nur³

2019

Bangla. J. Microbiol.

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The present study attempted to detect the effect of cold shock on Pseudomonas aeruginosa, P. fluorescence, and P. putida; to deduce the culturable cells, the possible dead cells and viable but non-culturable (VBNC) cells; i. e., living but not capable of forming the colony forming units (CFUs) at 0 oC and at 4 oC. Estimation of VBNC at low temperature was performed by deducting the number of culturable cells (formed as CFUs) at 0 °C and at 4 oC from the culturable cells at 37 °C. Maximum culturable cells (around 106 CFU/ ml) appeared at 24-48 hours, and after that, a gradual decline in the numbers of the culturable cells was observed. A minor but significant growth reduction was noticed in Minimal Broth compared to that in Luria Broth. Significant reduction in the culturable cells of all three strains was noticed under cold shock. However, a fraction of the population of each strain was observed to survive under cold stress. Interestingly while the number of culturable cells decreased in course of time under cold shock, the number of VBNC apparently seemed to be constant or to be increased. A comparatively lower number of VBNC was noticed under cold shock in case of P. putida which in turn affected culturability at ambient temperature. Bangladesh J Microbiol, Volume 34 Number 2 December 2017, pp 119-124

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Assessment of survival of pathogenic bacteria in fresh vegetables through in vitro challenge test

Cited by 10 publications

References 29 publications

Transmission of pathogens within the commonly consumed vegetables: Bangladesh perspective

Transmission of pathogens within the commonly consumed vegetables: Bangladesh perspective

Microbiological analysis for drug resistant pathogenic microorganisms with determination of the antibacterial properties found in Fragaria x ananassa (strawberry) samples

In Vitro demonstration of Pseudomonas Growth and Phenotypic Examinations of the Cells Under Cold Shock

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