Microbiological analysis for drug resistant pathogenic microorganisms with determination of the antibacterial properties found in Fragaria x ananassa (strawberry) samples

Piangka, Nazneen; Ahmed, Tasnia; Acharjee, Mrityunjoy

doi:10.3329/sjm.v6i1.33512

Cited by 2 publications

(2 citation statements)

References 27 publications

(34 reference statements)

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“…Moreover, strawberries have antimicrobial effects, especially against Pseudomonas sp. [ 31 ]. Sea buckthorn ( Hippophae rhamnoides L.) fruits are an important source of flavonoids (rutin, quercetin, kaempferol, myricetin), vitamins (E, C, B1, B2, B9 and K), carotenoids, amino acids, proteins, and minerals (K, Ca, Fe and P).…”

Section: Introductionmentioning

confidence: 99%

Geographic Variability of Berry Phytochemicals with Antioxidant and Antimicrobial Properties

et al. 2022

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The aim of this study was to determine the variability of several chemical compounds and the antioxidant and antimicrobial activities of eight types of berries harvested from two different geographical regions in the same year. The analyses were performed on bilberry, black currant, gooseberry, red currant, raspberry, sea buckthorn, strawberry and sour cherry, which were handpicked during the summer of 2019, in the same periods when they are typically harvested for consumer purposes. Total anthocyanins content (TAC), total flavonoids content (TFC), total polyphenolic compounds (TPC), determination of the Ferric-Reducing Antioxidant Power (FRAP), determination of the DPPH free radical scavenging assay (RSA), determination of nine phenolic compounds by HPLC-UV assay and antimicrobial activity were determined for undiluted hydroalcoholic extracts of all the studied berries. The results showed that the berries from Romania were richer in antioxidant compounds than the berries from Russia. The TPC content varied between 4.13–22.2 mg GAE/g d.w., TFC between 3.33–8.87 mg QE/g d.w. and TAC between 0.13–3.94 mg/g d.w. The highest variability was determined for TPC. Regarding the antioxidant activity assessed by FRAP assay, values were between 6.02–57.23 µmols TE/g d.w. and values for the RSA method between 18.44–83.81%. From the eight types of berries analyzed, bilberries and raspberries had the highest antioxidant activity considering both regions and both determination methods. Not only the type, but also the environmental and cultivation conditions in which the berries grow, can lead to variations in their chemical composition. The extracted polyphenolic compounds from the studied berries showed antibacterial properties on pathogens, such as Escherichia coli, Bacillus subtilis and Staphyloccocus aureus. The inhibitory action on Salmonella typhi and fungi Candida albicans and Aspegillus niger was absent to very low. The antimicrobial activity of the hydroalcoholic extracts was dependent on the provenance of the berries, too.

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Section: Introductionmentioning

confidence: 99%

Geographic Variability of Berry Phytochemicals with Antioxidant and Antimicrobial Properties

et al. 2022

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“…For microbiological analysis fresh juice samples were homogenized and serially diluted to 10 -6 in normal saline 10 . 0.1 ml of diluted juice samples were introduced and spread plated onto nutrient agar (NA) media for enumerating total viable bacteria (TVB) and Sabouraud Dextrose Agar (SDA) for Total Fungal Count (TFC), respectively 11 . For identification and enumeration of specific pathogenic bacteria 0.1ml of diluted fresh juice samples were added and spread onto MacConkey Agar (MA), Mannitol Salt Agar (MSA) and Cetrimide Agar (CA) media for selective isolation of Escherichia coli, Staphylococcus spp.…”

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Study of Microbiological Quality of Fresh Juices Vended in Dhaka City

Afroz

Shammi

Kabir³

2019

Bangla. J. Microbiol.

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Fresh juices sold by the street vendors are rich in nutritional value and considered as popular drink in Dhaka city .They can be easily contaminated by pathogenic bacteria from unhygienic environment of food preparation area and serves as a potential source of food borne outbreaks. Freshly produced juices of papaya (n = 2), wood apple (n = 2), wood apple and papaya juice (1), lemon (n = 2), and sugarcane (n = 3) were examined for the total bacterial mload, coliforms, Staphylococcus spp., Pseudomonas spp., Vibrio spp., Salmonella spp., and Shigella spp. in this study. Total bacterial load in eight samples were within 0 to 107 cfu/ml, six samples exhibited the presence of staphylococci within the range of 0 to 105 cfu/ml. Total coliforms were detected in six samples which ranged from 104 to 105 cfu/ ml. Vibrio spp., Salmonella spp., and Shigella spp. were isolated by enrichment and selective plating method. Eight samples demonstrated the presence of Vibrio spp. and none of them were positive for Salmonella spp. and Shigella spp. Antibiotic sensitivity patterns were determined against eight isolates using Erythromycin (15μg), Amoxicillin (10 μg), Gentamicin (10 μg), Vancomycin (30 μg), Ciprofloxacin (5 μg), Rifampicin (5 μg), Azithromycin (15 μg), Neomycin (10 μg) and Cefexime (5 μg). Klebsiella sp. (n = 1), Vibrio sp. (n = 2), Enterobacter sp. (n = 1), and Proteus sp. (n = 1) showed multi drug resistance in this study. Contamination of street vended juices pose risks to consumers health and requires adequate attention of appropriate authority. Bangladesh J Microbiol, Volume 36 Number 1 June 2019, pp 49-53

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Microbiological analysis for drug resistant pathogenic microorganisms with determination of the antibacterial properties found in Fragaria x ananassa (strawberry) samples

Cited by 2 publications

References 27 publications

Geographic Variability of Berry Phytochemicals with Antioxidant and Antimicrobial Properties

Geographic Variability of Berry Phytochemicals with Antioxidant and Antimicrobial Properties

Study of Microbiological Quality of Fresh Juices Vended in Dhaka City

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