Assessment of salivary pro inflammatory cytokines profile level in patients treated with labial and lingual fixed orthodontic appliances

Baeshen, Hosam Ali

doi:10.1371/journal.pone.0249999

Cited by 9 publications

(14 citation statements)

References 28 publications

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“…The cytokine levels were measured in the culture supernatants of splenocytes, and in the collected saliva and tears as described before [ 9 , 17 , 18 ], with some modifications. In brief, the isolated splenocytes were suspended in RPMI 1640 medium supplemented with 10%FBS and 1%penicillin/streptomycin (100 U/ml,100μg/mL) and were stimulated with anti-CD3 and anti-CD28 at 37°C in a humidified 5%CO2 chamber for 72 hours.…”

Section: Methodsmentioning

confidence: 99%

Dental follicle mesenchymal stem cells ameliorated glandular dysfunction in Sjögren’s syndrome murine model

et al. 2022

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Objective Dental mesenchymal stem cells (MSCs) are potential for use in tissue regeneration in inflammatory diseases due to their rapid proliferating, multilineage differentiation, and strong anti-inflammatory features. In the present study, immunoregulatory and glandular tissue regeneration effects of the dental follicle (DF)MSCs in Sjögren’s Syndrome (SS) were investigated. Methods Dental follicle (DF) tissues were obtained from healthy individuals during tooth extraction, tissues were digested enzymatically and DFMSCs were cultured until the third passage. DFMSCs were labeled with Quantum dot 655 for cell tracking analysis. The induction of the SS mouse model was performed by the injection of Ro60-273-289 peptide intraperitoneally. DFMSCs were injected intraperitoneally, or into submandibular, or lacrimal glands. Splenocytes were analyzed for intracellular cytokine (IFN-γ, IL-17, IL-10) secretion in T helper cells, lymphocyte proliferation, and B lymphocyte subsets. Histologic analysis was done for submandibular and lacrimal glands with hematoxylin-eosin staining for morphologic examination. Results The systemic injection of DFMSCs significantly reduced intracellular IFN-γ and IL-17 secreting CD4+ T cells in splenocytes (p<0.05), and decreased inflammatory cell deposits and fibrosis in the glandular tissues. DFMSCs differentiated to glandular epithelial cells in submandibular and lacrimal injections with a significant reduction in lymphocytic foci. The results showed that few amounts of DFMSCs were deposited in glandular tissues when applied intraperitoneally, while high amounts of DFMSCs were located in glandular tissues and differentiated to glandular epithelial cells when applied locally in SS murine model. Conclusion DFMSCs have the potential for the regulation of Th1, Th17, and Treg balance in SS, and ameliorate glandular dysfunction. DFMSCs can be a beneficial therapeutic application for SS.

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Section: Methodsmentioning

confidence: 99%

Dental follicle mesenchymal stem cells ameliorated glandular dysfunction in Sjögren’s syndrome murine model

et al. 2022

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“…An increase in the salivary TNF-α level in pregnant women, especially those who are obese, can affect the weight of the infant [ 35 ]. On the other hand, tooth movement caused by orthodontic force may increase salivary TNF-α [ 36 ] and IL-6 levels [ 14 ]. It can be concluded that, in the absence of pregnancy, the concentration of salivary IL-6 and TNF-α increases due to orthodontic appliance use.…”

Section: Discussionmentioning

confidence: 99%

“…In relation to orthodontic treatment, IL-6 plays an important role in the movement of teeth, namely the inflammatory process that supports the resorptions process in apical areas [ 12 , 13 ]. Increased IL-6 levels occur not only in periodontal or alveolar tissues but also in saliva [ 14 ]. Similar to IL-6, TNF-α is also affected by orthodontic treatment and plays a role in the processes of tooth movement and bone resorption [ 15 ].…”

Section: Introductionmentioning

confidence: 99%

Oral Microbiome Profiles and Inflammation in Pregnant Women Who Used Orthodontic Appliances

et al. 2022

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It is common for women to undergo orthodontic treatment during pregnancy, especially through the use of fixed orthodontic devices. In changing the oral microbiome profile, it is crucial to increase the immune responses of pregnant women using fixed orthodontics; however, changes in the microbiomes of pregnant women with orthodontic appliances can be adjusted. Therefore, we aimed to conduct research on the oral cavity microbiome profiles, specifically IL-6 and TNF-α, of pregnant women using fixed orthodontic appliances. We proposed an observational analysis of 30 third-trimester pregnant women. OHI-S was recorded, saliva collection was performed using the passive drool method for IL-6 and TNF-α, and analysis and mucosal swabs were used to determine the oral microbiome profile. Kruskal–Wallis and post hoc Bonferroni tests were used to identify any significant differences with values of p < 0.05. Of these pregnant women, those with orthodontic appliances developed 10 types of bacteria at similar levels (>80%) from the genera Streptococcus, Lactobacillus, and Veillonella. There was no difference between the oral microbiomes of the control group and the pregnant women with a history of orthodontic appliance use. While the level of TNF-α in the women with orthodontic appliances was higher compared with the control group who had never used orthodontic appliances (p < 0.05), there was no difference in the IL-6 levels. The IL-6 and microbiome profile produced normal results, so the use of orthodontic appliances during pregnancy should be allowed with conditions. Pregnant women with orthodontic appliances must keep the oral cavity clean and their appliances well-maintained to avoid oral problems.

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“…The bead assay LEGENDplex™ Human Inflammation Panel 1 (13-plex) with V-bottom Plate (BioLegend, San Diego, CA, USA), previously used successfully with saliva samples [12,13], was used to quantify inflammatory markers (IL-1β, IFN-α2, IFN-γ, TNF-α, MCP-1, IL-6, IL-8, IL-10, IL-12p70, IL-17A, IL-18, IL-23, and IL-33) in oral samples from intervention and control groups.. Specifically, the supernatants obtained after sample centrifugation for 10 min at 10.000g, from the intervention (at baseline (M0), after 1 month of PR (M1), and after 3 months of PR (M3)) and control groups (in the timepoints M0, M1 and M3), were used for cytokine quantification following manufacturer’s recommendations [14].…”

Section: Methodsmentioning

confidence: 99%

“…Moreover, the oral and lower airway microbiotas are highly correlated [10], given the topological continuity between the niches, implying oral bacteria as the major colonizers of the lungs through aspiration [10][11][12].…”

Section: Introductionmentioning

confidence: 99%

Responsiveness to pulmonary rehabilitation in COPD is associated with changes in microbiota

Dias

Cabral

Furtado

et al. 2022

Preprint

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Rationale: Pulmonary Rehabilitation (PR) is one of the most cost-effective therapies for chronic obstructive pulmonary disease (COPD) management. There are, however, people who do not respond to PR and reasons for non-response are mostly unknown. PR is likely to change the airway microbiota and this could play a role in its responsiveness. Objectives: In this study we have explored the association between PR effectiveness and specific alterations in oral microbiota and inflammation. Methods: A prospective longitudinal study was conducted. Data on exercise capacity, dyspnoea, impact of disease and 418 saliva samples were collected from 76 patients, half of whom participated in a 12-weeks PR programme. Responders and non-responders to PR (dyspnoea, exercise-capacity and impact of disease) were defined based on minimal clinically important differences. Measurements and Main Results: Changes in microbiota, including Prevotella melaninogenica and Streptococcus were observed upon PR. Prevotella, previously found to be depleted in severe COPD, increased during the first month of PR in responders. This increase was negatively correlated with Streptoccus and Lautropia, known to be enriched in severe cases of COPD. Simultaneously, an anti-inflammatory commensal of the respiratory tract, Rothia, correlated strongly and negatively with several pro-inflammatory markers, whose levels were generally boosted by PR. Conversely, in non-responders, the observed decline in Prevotella correlated negatively with Streptococcus and Lautropia whose fluctuations co-occurred with several pro-inflammatory markers. Conclusions: PR is associated with changes in oral microbiota. Consistent patterns of bacteria/pro-inflammatory markers among responders and non-responders were unraveled, which may contribute to explain the success of PR.

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Assessment of salivary pro inflammatory cytokines profile level in patients treated with labial and lingual fixed orthodontic appliances

Cited by 9 publications

References 28 publications

Dental follicle mesenchymal stem cells ameliorated glandular dysfunction in Sjögren’s syndrome murine model

Dental follicle mesenchymal stem cells ameliorated glandular dysfunction in Sjögren’s syndrome murine model

Oral Microbiome Profiles and Inflammation in Pregnant Women Who Used Orthodontic Appliances

Responsiveness to pulmonary rehabilitation in COPD is associated with changes in microbiota

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