Dental follicle mesenchymal stem cells ameliorated glandular dysfunction in Sjögren’s syndrome murine model

Abstract

Objective Dental mesenchymal stem cells (MSCs) are potential for use in tissue regeneration in inflammatory diseases due to their rapid proliferating, multilineage differentiation, and strong anti-inflammatory features. In the present study, immunoregulatory and glandular tissue regeneration effects of the dental follicle (DF)MSCs in Sjögren’s Syndrome (SS) were investigated. Methods Dental follicle (DF) tissues were obtained from healthy individuals during tooth extraction, tissues were digested enzymatically and DFMSCs were cultured until the third passage. DFMSCs were labeled with Quantum dot 655 for cell tracking analysis. The induction of the SS mouse model was performed by the injection of Ro60-273-289 peptide intraperitoneally. DFMSCs were injected intraperitoneally, or into submandibular, or lacrimal glands. Splenocytes were analyzed for intracellular cytokine (IFN-γ, IL-17, IL-10) secretion in T helper cells, lymphocyte proliferation, and B lymphocyte subsets. Histologic analysis was done for submandibular and lacrimal glands with hematoxylin-eosin staining for morphologic examination. Results The systemic injection of DFMSCs significantly reduced intracellular IFN-γ and IL-17 secreting CD4+ T cells in splenocytes (p<0.05), and decreased inflammatory cell deposits and fibrosis in the glandular tissues. DFMSCs differentiated to glandular epithelial cells in submandibular and lacrimal injections with a significant reduction in lymphocytic foci. The results showed that few amounts of DFMSCs were deposited in glandular tissues when applied intraperitoneally, while high amounts of DFMSCs were located in glandular tissues and differentiated to glandular epithelial cells when applied locally in SS murine model. Conclusion DFMSCs have the potential for the regulation of Th1, Th17, and Treg balance in SS, and ameliorate glandular dysfunction. DFMSCs can be a beneficial therapeutic application for SS.

Regenerative Effect of Lyophilized Mesenchymal Stem Cells Combined with Platelet-Rich Fibrin in a Skin Wound Model in Geriatric and Young Rats

“…In previous studies, it was reported that freshly prepared MSCs can differentiate into non-mesodermal cell types such as epithelial, hepatocyte, and renal tubular cells. 23,24 The data showed that lyophilized DF-MSCs can differentiate to epithelial cells after being transferred to the wound site.…”

Management of Sjogren’s Dry Eye Disease—Advances in Ocular Drug Delivery Offering a New Hope

“…Dental pulp-derived stem cells may improve Sjogren’s hyposecretion through the TGF-b/Smad pathway, and studies suggest it may be more effective than bone marrow MSCs [ 78 , 79 ]. Dental follicle MSC decreased inflammatory cell deposits and fibrosis in glandular tissue, and showed differentiation into glandular epithelial cells [ 80 ]. Human exfoliated deciduous teeth stem cells, which are from the cranial neural crest, secrete soluble programmed cell death ligand 1 (PD-L1) and alleviate gland inflammation and dryness in mice [ 81 ].…”

Effective-mononuclear cell (E-MNC) therapy alleviates salivary gland damage by suppressing lymphocyte infiltration in Sjögren-like disease

“…Over the past decade, cell-based therapies have been examined as potential therapeutic approaches for SS ( Khalili et al, 2014 ; Elghanam et al, 2017 ; Genç et al, 2022 ). A particularly promising therapeutic option is the use of mesenchymal stem cells (MSCs).…”