Assessment of random amplified polymorphic DNA (RAPD) as genetic markers for determining the origin of interspecific lilac hybrids

Abstract

Summary Marsolais, J. V., Pringle, J. S. & White, B. N.: Assessment of random amplified polymorphic DNA (RAPD) as genetic markers for determining the origin of interspecific lilac hybrids. – Taxon 42: 531–537. 1993. – ISSN 0040‐0262. RAPD markers were used to assess the relationship among species, cultivars and hybrids of lilacs. Thirteen random primers were used to examine 87–130 bands per cultivar or hybrid. The percent band sharing among Syringa xchinensis ‘Alba’, S. xchinensis ‘Saugeana’, S. xchinensis ‘Red Rothomagensis’ and S. xpersica, was surprisingly high (98.3 % to 99.6 %), supporting the theory that S. xpersica has the same species parentage as the three S. xchinensis hybrids, but also indicating that all four are of different clonal origin since they do not share 100 % of their bands. The percent band sharing among the hybrids and their putative parental species, S. vulgaris L. (3 selections) and S. protolaciniata P. S. Green & M. C. Chang (2 selections), were also determined. On average the first shared 48.9 % of their bands with the hybrids, and the second shared 50.7 %, thus strongly supporting the postulated parentage. Most bands found in the hybrids were shared with at least one of the selections, but 11 bands were found exclusively in all four hybrids. They might either represent genetic characteristics specific to these hybrids or might indicate that the exact parental selection combination has not yet been studied. The two studied selections of S. protolaciniata shared only 90,7 % of their bands, thus confirming that they are of different provenances. It is felt that RAPD markers will be very useful for assessing the origin of cultivars and hybrids of many plant species.

An Investigation of RAPD Analysis of Genetic Variability of Some Rice Germplasm (Oryza sativa L.)

“…The present investigation revealed the effectiveness of RAPD in detecting polymorphism among different 27 cultivars of rice. The success of RAPD analysis in O. sativa accessions were also reported earlier (Muhammad et al, 2005;Rahman et al, 2007;Malik et al, 2008). The percentage of polymorphism was found near to be 95 %.…”

“…Information on intraspecific variation from the present study might be useful in making decision for improvement of rice cultivars. Similarity level up to 80% in cluster analysis is indicative of plant derived from interspecific hybridization (Marsolais et al, 1993).…”

Molecular Markers as a Tool for Analyses of Genetic Relatedness and Selection in Ornamentals

“…SUdbeck and Wolff Wolff and Peters-Van Rijn, 1993;Wolff et al, 1995;Scott et al, 1996;Wolff, 1996Vainstein et al, 1991Ling et al, 1997Starman et al, 1999Tzuri et al, 1991Vainstein et al, 1993Goh et al, 1995Kumar et al, 1998Adams and Demeke, 1993Yamagishi, 1995Faccioli et al, 2000Ko et al, 1996Rmou et al, 1997Starman and Abbit, 1997;Barcaccia et al, 1999;Becher et al, 2000;Lesur etal., 2000, Cerny et al, 1996Jianhua et al, 1997 Dunemarm andKahnau, 1998;De Riek et al, 1999 ;Naito et al, 1999Tzuri et al, 1991Hubbard et al, 1992;Rajapakse eta/., 1992;Torres eta/., 1993;Vainstein et al, 1993;Vainstein and Ben Meir, 1994;;Dehmer et al, 1996;Gallego and Martinez, 1996;Matsumoto and Fukuri, 1996;Millan et al, 1996Dehmer et al, 2000Swoboda and Balla, 1997Marsolais et al, 1993 …”

Genetic variations of Lansium domesticum Corr. accessions from Java, Sumatra and Ceram based on Random Amplified Polymorphic DNA fingerprints

“…0.55-0.69). Marsolais et al (1993) suggested that the range of 0.50 using RAPD may be implied the occurrence of interspecific hybrid, while range between 0.61-0.99 could suggest genetic similarity at the species level in Lilac. While interspecific hybrid in Mentha spicata and M. arvensis shared 56 and 49% similarity to the parents (Shasany et al 2005).…”