An Investigation of RAPD Analysis of Genetic Variability of Some Rice Germplasm (Oryza sativa L.)

Roopa, B.R.; Chikkaswamy, B. K.

doi:10.20546/ijcmas.2016.509.095

Cited by 1 publication

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References 9 publications

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“…(2011) found high genetic diversity among the 30 rice genotypes tested in which the pair-wise estimates of similarity ranged from 0.64 to 0.94. Roopa and Chikkaswamy (2016) and Rabbani et al. (2008) also found high genetic diversity among tested cultivars in which genetic similarity coefficients ranged from 0.46 to 0.91 and 0.50 to 0.96 respectively.…”

Section: Discussionmentioning

confidence: 83%

“…(2011) reported 68.94% polymorphism with 20 RAPD primers among 30 salinity tolerant rice cultivars and a total of 161 bands were produced. Moreover, a variety of other researchers observed different level of polymorphism, 80% polymorphism among 42 Indian elite varieties was revealed by using 40 RAPD primers ( Davierwala et al., 2000 ), 96.2% polymorphism was reported among 27 selected Indian rice genotypes using 30 decamer RAPD primers ( Roopa and Chikkaswamy, 2016 ), RAPD analysis in 10 traditional, 28 improved and 2 Japanese cultivars of rice using 25 random primers revealed 89.4% polymorphism ( Rabbani et al., 2008 ) and 80% polymorphism was observed among 9 upland and 4 lowland indica and japonica rice cultivars using 42 RAPD primers ( Yu and Nguyen, 1994 ). Choudhury et al.…”

Section: Discussionmentioning

confidence: 99%

“…These primers were obtained from Integrated DNA Technologies, Inc. (Coralville, IA, U.S.). These primers were selected based on their highly polymorphic nature that has been observed in previous study of various groups of researchers although the polymorphism content of RAPD primers vary with the cultivars ( Davierwala et al., 2000 ; Kanawapee et al., 2011 ; Choudhury et al., 2001 ; Roopa and Chikkaswamy, 2016 ; Kiani and Katalani, 2018 ; Ravi et al., 2003 ).…”

Section: Methodsmentioning

confidence: 99%

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Genetic variability analysis of partially salt tolerant local and inbred rice (Oryza sativa L.) through molecular markers

Mazumder

Hoque

Sinha

et al. 2020

Heliyon

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Random Amplified Polymorphic DNA (RAPD) analysis was performed to assess the genetic variability in sixteen selected germplasms of rice, Oryza sativa L. using eight decamer RAPD primers. The data obtained from this investigation reveals a high level of polymorphism between cultivars. The primers produced a total of 255 bands of which all 255 bands were polymorphic indicating 100% polymorphism. The size of the amplified bands ranged from 220 bp to 2290 bp. The number of polymorphic fragments ranged from 24 to 49 with an average of 32 polymorphic fragments for each primer. The primer OPX04 produced the maximum number (49) of polymorphic bands while the OPB04 and OPB17 produced the minimum number (24) of polymorphic bands. The polymorphic information content (PIC) values ranged from 0.6616 to 0.8845 with an average of 0.832. The highest PIC value (0.8845) was obtained for primer OPL03. The RAPD data was analyzed to determine the pair-wise genetic similarity coefficients which ranged from 0.00 to 0.83. The BRRIdhan 23 and the BRRIdhan 41 varieties were the closest genotypes with the highest similarity index of 83%. This was followed by 77% similarity between a pair of cultivars Kalamona and Horkuch. On the other hand, 100% dissimilarity was seen between BRRIdhan 53, BRRIdhan 50, BRRIdhan 10, BRRIdhan 70, BRRIdhan 54, BRRIdhan 40, BRRIdhan 23, BRRIdhan 47, BRRIdhan 41 and Dadsail respectively and between BRRIdhan 53 and Horkuch; indicating a high level of variability between paired genotypes. Cluster analysis was performed using Unweighted Paired Group of Arithmetic Means (UPGMA). The UPGMA dendrogram resolved the selected rice cultivars into four clusters.

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Section: Discussionmentioning

confidence: 83%