Assessment of proteinuria using random urine samples

Houser, Mark T.

doi:10.1016/s0022-3476(84)80478-3

Cited by 132 publications

(55 citation statements)

References 11 publications

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“…Hemmingsen and Skaarup reported that the recovery was 102% in serum and 96% in urine. The intra-day assay variation coefficient was 7.1% in serum and 3.8% in urine [7]. In comparison, our assay was accurate enough to give similar results.…”

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confidence: 51%

“…There was a close correlation between the β 2 -m creatinine index of random urine samples and that of 24 hr urine samples (r= 0.872). It was reported that urinary protein excretion in random urine samples closely represented of daily excretion, when factored by creatinine [7]. No daily variation in the serum and urinary β 2 -m concentration was observed [8,10].…”

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confidence: 99%

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Determination of Canine .BETA.2-Microgloblin in Plasma and Urine by Enzyme-linked Immunosorbent Assay.

Nakajima

Hoshi

Higuchi

et al. 2001

The Journal of Veterinary Medical Science

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ABSTRACT. An enzyme-linked immunosorbent assay was developed for the determination of canine β 2 -microglobulin (β 2 -m) in plasma and urine. The detectable sensitivity for pure canine β 2 -m was 0.05 µg/l and the analytical range was 0.1 to 50 µg/l. The mean analytical recovery when pure canine β 2 -m was added to normal plasma was 101.9%. The mean analytical recovery in the urine was 102.1%. The intra-day variation coefficient was 3.1% in plasma, 4.3% in serum and 1.9% in urine. No difference was found between the concentration of β 2 -m in plasma and serum (n=17). The concentration of β 2 -m in the plasma of normal dogs was 1.82 ± 0.57 mg/l (n=31). The mean excretion in 24 hr urine collected from normal dogs was 17.6 ± 9.2 µg/l, 0.22 ± 0.12 µg/kg of body weight or 14.2 ± 9.4 µg/g of urine creatinine. The β 2 -m creatinine index of random urine samples was 23.5 ± 16.6 µg/g (n=26). There was a close correlation between the β 2 -m creatinine index of 24 hr urine samples and that of random urine samples (r= 0.872). KEY WORDS: canine, ELIS, β 2 -microglobulin.J. Vet. Med. Sci. 63(3): 343-345, 2001 β 2 -microglobulin (β 2 -m) is a low molecular weight protein that is present on the surface of all nucleated cells [12]. This protein is the light chain of the class I major histocompatibility antigens [13]. Determination of serum β 2 -m and the urine β 2 -m concentration is used to evaluate renal function in man [3,9]. The determination of β 2 -m has been carried out by radioimmunoassay [4], enzyme-linked Immunosorbent assay (ELISA) [2] and immunoturbidimetry [1], but there are no reports on the measurement of β 2 -m concentrations in dog body fluids. In this study, we developed an ELISA test for determination of β 2 -m in the plasma, serum and urine of dogs.Purified canine β 2 -m and antiserum were prepared in our laboratory. The protocol used for isolation of canine β 2 -m from the urine was reported previously [11]. For preparation of antiserum, a rabbit was immunized by the injection of 1 mg of β 2 -m with complete Freund's adjuvant (ORGA-NON TECHNIKA Co., Belgium) intracutaneously into its back. The rabbit was injected every three weeks for a total of 3 times. The IgG fraction of the antiserum was precipitated with ammonium sulfate and then purified by means of an Econo-Pac Protein A Cartridge (Bio-Rad Laboratories, U.S.A.) according to Tarditi et al. [14]. The purified IgG was dialyzed against ammonium acetate buffer (pH 8.6, 4°C), lyophilized and stored at 4°C. The IgG fraction was conjugated with biotin (biotinyl-N-hydroxysuccinimide ester, EY Laboratories, U.S.A.) by a method similar to that of Guesdon et al. [5].Blood samples were obtained from 32 normal dogs. Plasma was isolated from 3 ml of whole blood, with heparin as anticoagulant. In addition, simultaneous serum samples were taken from 17 dogs. Twenty-four hour urine samples and random urine samples were collected from 26 normal dogs, and each urine sample was stored in several PE-tubes. Five µl of Tween 20 (Kantokagaku Co., Ltd., Japan) was added to ...

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confidence: 51%

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confidence: 99%

Determination of Canine .BETA.2-Microgloblin in Plasma and Urine by Enzyme-linked Immunosorbent Assay.

Nakajima

Hoshi

Higuchi

et al. 2001

The Journal of Veterinary Medical Science

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“…Other investigators, using similar tech¬ niques, have found correlations in pediatrie populations and in adults with systemic lupus erythematosus. 7,8 This study represents a prospective comparison of pro¬ tein-to-creatinine ratios in single-voided specimens with 24-hour urine protein collections in the assessment of proteinuria. This clinical trial significantly expands previ¬ ous observations by the inclusion of ambulatory and immo¬ bilized (bed-bound) patients, and by the prospective selec¬ tion of a diverse patient population (Table).…”

Section: Commentmentioning

confidence: 99%

Quantitation of Proteinuria by the Use of Protein-to-Creatinine Ratios in Single Urine Samples

Schwab¹

1987

Arch Intern Med

184

104

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“…Up/c has been shown to reflect 24-h urine protein collection quite accurately [1], and it is recommended to quantify Up/c in the first-voided morning urine to avoid the effect of exercise and orthostatics [17]. Twenty-four-hour urine protein measurement is susceptible to over-collection or under-collection errors and commonly restricted to toilet trained children with elevated Up/c or Ualb/c ratio in random samples.…”

Section: Urinary Protein Quantificationmentioning

confidence: 99%

Clinical practice

Ariceta

2010

Eur J Pediatr

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Proteinuria detection in children is a challenge. Five percent to 15% and 0.4-1% of school children present either transient (benign) or persistent increased amount of protein in urine, respectively. Persistent proteinuria constitutes not only a sign of overt kidney disease but may also be the first indicator of silent renal damage. Proteinuria is a marker for hyperfiltration in individuals with reduced nephron mass and one of the most important independent risk factor for renal disease progression as well. It constitutes the single most important risk factor for future loss of kidney function, preceding glomerular filtration rate reduction. Further, proteinuria itself is diagnostic of cardiovascular disease with prognostic value and target organ involvement in high-risk populations such as diabetic, obese, hypertensive children, or those with known reduced renal mass or previous renal injury. Current strategies to prevent CKD progression, a concept known as renoprotection, are focused on reducing urinary protein excretion among other factors. Reversibility of organ damage in early stages is possible; therefore, pediatricians should screen children for proteinuria or microalbuminuria, mainly in high-risk groups.

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Assessment of proteinuria using random urine samples

Cited by 132 publications

References 11 publications

Determination of Canine .BETA.2-Microgloblin in Plasma and Urine by Enzyme-linked Immunosorbent Assay.

Determination of Canine .BETA.2-Microgloblin in Plasma and Urine by Enzyme-linked Immunosorbent Assay.

Quantitation of Proteinuria by the Use of Protein-to-Creatinine Ratios in Single Urine Samples

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