Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay

Duarte, Sabrina Castilho; Linhares, Guido Fontgalland Coelho; Romanowsky, Tatiana Nunes; Neto, Osvaldo José da Silveira; Borges, Lígia Miranda Ferreira

doi:10.1016/j.vetpar.2007.12.013

Cited by 53 publications

(44 citation statements)

References 28 publications

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“…Similarities of between 99.4 and 100% were reported on the basis of B. canis vogeli 18S rRNA gene analyses on isolates from five dogs in the states of Minas Gerais and São Paulo (PASSOS et al, 2005). B. canis vogeli has also been identified in samples from Rio de Janeiro by means of RFLP-PCR (DE SÁ et al, 2006) and from Goiás by means of a novel subspecies-specific PCR assay (DUARTE et al, 2008a Morphological examination of blood smears revealed small piroplasms similar to B. gibsoni in one dog in southern Brazil (BRACCINI et al, 1992) but this finding was not confirmed by means of any molecular technique. Based on sequence analysis on a partial fragment of the 18S rRNA gene, B. gibsoni genotype Asia 1 was characterized from four dogs in southern Brazil (TRAPP et al, 2006).…”

Section: Discussionmentioning

confidence: 57%

“…The PCR mix was prepared for a final volume of 50 µL, as described by Duarte et al (2008a), as follows: 1× PCR buffer (Invitrogen, Carlsbad, USA); 2.0 mM magnesium chloride (Invitrogen); 0.2 mM dNTP (GE Healthcare, Buckinghamshire, England); 10 pM forward primer Bab7; 10 pM reverse primer Bab9; 1.25 U of Taq DNA polymerase (Invitrogen) and 5 µL of DNA sample. Amplification was performed in a thermocycler (Eppdendorf Mastercycler Personal, Hamburg, Germany), programmed for 35 cycles of denaturation at 94 °C for 30 seconds, annealing at 56 °C for 30 seconds and extension at 72 °C, preceded by an initial denaturation for 2 minutes and followed by a final elongation at 72 °C.…”

Section: Pcr Assaymentioning

confidence: 99%

“…Babesia gibsoni is predominantly found in Asia, Africa, North America, Europe and Australia (EIRAS et al, 2008;IRWIN, 2009). In Brazil, the most common piroplasm in dogs is B. canis vogeli (PASSOS et al, 2005;DUARTE et al, 2008a;RAMOS et al, 2010). However, there have been two reports on the presence of B. gibsoni in southern Brazil (TRAPP et al, 2006).…”

Section: Introductionmentioning

confidence: 99%

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Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil

Duarte

Parente

Pereira

et al. 2011

Rev. Bras. Parasitol. Vet.

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The genus Babesia comprises protozoa that cause diseases known as babesiosis. Dogs are commonly affected by Babesia canis or Babesia gibsoni. Babesia canis is divided into the subspecies Babesia canis canis, Babesia canis vogeli and Babesia canis rossi. Among these, Babesia canis vogeli predominates in Brazil. The objective of this study was to conduct a phylogenetic analysis on Babesia isolates from dogs in Goiânia, Goiás. Blood samples were obtained from 890 dogs presenting clinical signs suggestive of canine babesiosis that were attended at a veterinary hospital of Goiás. Only samples presenting typical intraerythrocytic parasites were used in the study. These were subjected to DNA extraction and amplification of a fragment of the 18S rRNA, by means of PCR. The PCR products were purified and sequenced. Sequences were obtained from 35 samples but only 17 of these were kept after quality assessment. Similarity analysis using BLASTn demonstrated that all 17 sequences corresponded to B. canis vogeli. Analysis using the Mega4 software showed that the isolates of B. canis vogeli from dogs in Goiânia present a high degree of molecular similarity (99.2 to 100%) in comparison with other reference isolates from other regions of Brazil and worldwide, deposited in GenBank.Keywords: Babesia canis vogeli, canine babesiosis, molecular characterization, phylogeny. Resumo

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Section: Discussionmentioning

confidence: 57%

Section: Pcr Assaymentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil

Duarte

Parente

Pereira

et al. 2011

Rev. Bras. Parasitol. Vet.

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“…DNA samples were tested by means of the polymerase chain reaction (PCR) using the sets of primers described in Table 1. For Babesia spp., a single PCR was used (DUARTE et al, 2008); for Ehrlichia canis, a nested PCR was performed, with the second reaction specific for E. canis (DAWSON et al, 1996;MURPHY et al, 1998). Reactions were performed in a final volume of 25 µl, containing 10 mM of Tris-HCl (pH 8.3), 50 µM of KCl, 1.5 mM of MgCl 2 , 0.2 mM of each deoxynucleoside triphosphate, 1.5 U of Taq DNA polymerase (Invitrogen), 11 pmol of each primer and approximately 100 ηg of canine genomic DNA.…”

Section: Methodsmentioning

confidence: 99%

A serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão, northeastern Brazil

Costa

Labruna

et al. 2015

Rev. Bras. Parasitol. Vet.

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This study evaluated exposure and infection by tick-borne agents (Babesia vogeli, Ehrlichia canis and Rickettsia spp.) in 172 dogs in rural areas and 150 dogs in urban areas of the municipality of Chapadinha, state of Maranhão, northeastern Brazil, using molecular and serological methods. Overall, 16.1% of the sampled dogs (52/322) were seroreactive to B. vogeli, with endpoint titers ranging from 40 to 640. For E. canis, 14.6% of the dogs (47/322) were seroreactive, with endpoint titers from 80 to 163,840. Antibodies reactive to at least one of the five species of Rickettsia were detected in 18.9% of the dogs (61/322), with endpoint titers ranging from 64 to 4,096. High endpoint titers were observed for Rickettsia amblyommii. Three (0.9%) and nine (2.8%) canine blood samples were PCR-positive for Babesia spp. and E. canis. The ticks collected from urban dogs were all Rhipicephalus sanguineus sensu lato, whereas the rural dogs were infested by R. sanguineus s.l, Amblyomma cajennense sensu lato and Amblyomma ovale. One A. ovale tick was found to be infected by Rickettsia bellii. This study provides an epidemiological background for controlling and preventing canine tick-borne diseases in a neglected region of Brazil.Keywords: Ticks, tick-borne pathogens, hemoparasites. ResumoEste estudo avaliou por métodos sorológicos e moleculares a exposição e infecção por agentes transmitidos por carrapatos (Babesia vogeli, Ehrlichia canis, and Rickettsia spp.)

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“…Olguda inguinal bölgede 3 adet keneye rastlanırken, hastanın çevreye karşı ilgisinin azaldığı, mukozalarında hafif ikterusla birlikte solgunluk ve yüksek ateşi (40.6ºC) olduğu belirlendi. Hematolojik muayenede RBC, Hb, HCT değerlerinde hafif [15] …”

Section: Olgu IIIunclassified

Türkiye’de Köpeklerde Babesia canis canis’in Klinik ve Parazitolojik Olarak İlk Tespiti

Kırmızıgül¹,

Taşçi²,

Uzlu³

et al. 2013

Kafkas Univ Vet Fak Derg

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Assessment of primers designed for the subspecies-specific discrimination among Babesia canis canis, Babesia canis vogeli and Babesia canis rossi by PCR assay

Cited by 53 publications

References 28 publications

Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil

Phylogenetic characterization of Babesia canis vogeli in dogs in the state of Goiás, Brazil

A serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão, northeastern Brazil

Türkiye’de Köpeklerde Babesia canis canis’in Klinik ve Parazitolojik Olarak İlk Tespiti

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