A serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão, northeastern Brazil

Costa, Andréa Pereira da; Costa, Francisco Borges; Labruna, Marcelo Bahia; Silveira, Iara; Moraes‐Filho, Jonas; Soares, João Fábio; Spolidorio, Mariana Granziera; Guerra, Rita de Maria Seabra Nogueira de Candanedo

doi:10.1590/s1984-29612015008

Cited by 27 publications

(28 citation statements)

References 58 publications

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“…The prevalence value recorded in this study is higher than 4.3% recorded in hunting dogs in Italy (Ebani et al, 2015) and 16.1% in rural dogs in Brazil (Costa et al, 2015). The different climatic and environmental conditions of the hunting dogs might be the possible reasons for the differences in the prevalence.…”

Section: Discussioncontrasting

confidence: 60%

Prevalence of Babesia species in hunting dogs in Ogun State South West Nigeria

Akande¹,

Adebowale²,

Takeet³

et al. 2017

AJVS

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Key words:Hunting dogs, Babesia species, prevalence, Microscopy, PCR.Dogs are commonly used for hunting in Nigeria and they may harbour parasites without showing any clinical features. Babesiosis is a worldwide, tick-borne, protozoal haemoparasitic disease caused by parasites of genus Babesia. This study assessed by means of microscopy and Polymerase Chain Reaction (PCR), the presence of Babesia species in 109 indigenous Nigerian hunting dogs. One hundred and nine hunting dogs were sampled from 21 different locations, from May 2014 to March 2015 with ages ranging from less than 1 to greater than 6 years. The average weight of the dogs studied was 12.20±0.33kg with the mean Packed Cell Volume (PCV) of 35.2±0.86% and mean age of 3.01±0.23 years. Using microscopy 24 (22%) of the dogs were positive for Babesia species. There were no statistically significant differences in the gender (p=0.84), age (p= 0.71), weight (p= 0.45) and PCV (p=0.87) of infected and non-infected dogs. With PCR assay, 52 (47.7%) of the sampled dogs were positive to Babesia spp. Of which 18 (34.6%) were male and 34(65.4%) were females. There were no statistical significance between the presence of Babesia spp. and sex (p=0.11) also with PCV (p=0.06) of hunting dogs but there were significant differences in their ages (p=0.04) and weights (p= 0.01). Using PCR as a gold standard the specificity and sensitivity of microscopy for detecting Babesia were 77.2% and 21.2 % respectively. The positive predictive value for microscopy was 45.8% while the negative predictive value was 51.8% with a diagnostic accuracy of 50%. The implication of the presence of the parasite in the study areas is discussed.

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Section: Discussioncontrasting

confidence: 60%

Prevalence of Babesia species in hunting dogs in Ogun State South West Nigeria

Akande¹,

Adebowale²,

Takeet³

et al. 2017

AJVS

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“…In Brazil, B. vogeli and B. gibsoni are the species identified in dogs so far (Passos et al, 2005;Trapp et al, 2006;Dantas-Torres and Otranto, 2014); nevertheless, the vector for B. gibsoni has not been detected in Brazil yet (Trapp et al, 2006). On the other hand, R. sanguineus s.l.is widely distributed in Brazil and adapts well to urban areas, which may contribute towards the endemic status of babesiosis in some Brazilian regions (Costa et al, 2015). Furthermore, dog-specific Babesia species have been sporadically detected by molecular methods in cats, also in Brazil, where new Babesia spp.…”

Section: Discussionmentioning

confidence: 99%

Identification of vector-borne pathogens in dogs and cats from Southern Brazil

Malheiros

Costa

Amaral

et al. 2016

Ticks and Tick-borne Diseases

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Dogs and cats are often infected with vector-borne pathogens and play a crucial role as reservoirs and hosts in their life cycles. The aim of the present study was to investigate the occurrence of vector-borne pathogens among dogs and cats in the northwestern region of Rio Grande do Sul (RS) State, Brazil. One hundred and ten blood samples were collected from dogs (n=80) and cats (n=30). Laboratory analysis were carried out through stained blood smears, indirect enzyme-linked immunosorbent assay (ELISA) for Babesia vogeli and Ehrlichia canis (only for dogs) and polymerase chain reaction (PCR) aiming the detection of pathogens. The following pathogens were screened by PCR among dogs and cats: Babesia spp. and Hepatozoon spp. (18S rRNA gene), Anaplasma spp. (16S rRNA gene), and Ehrlichia spp. (dsb gene for dogs and 16S rRNA gene for cats) and Bartonella spp. (nuoG gene only for cats). Using blood smears structures morphologically compatible with piroplasms were found in 5.45% (6/110) of the samples. Anti-B. vogeli and anti-E. canis antibodies were detected in 91% (73/80) and 9% (7/80) of the dogs, respectively. All the seropositive dogs to E. canis were also to B. vogeli. Nineteen (17.3%) animals were positive to hemoparasites by PCR. After sequencing Rangelia vitalii 6/80 (7.5%), B. vogeli 3/80 (4%), Hepatozoon spp. 1/80 (1%), and Anaplasma spp. 1/80 (1%) were found in the dogs, and B. vogeli 2/30 (7%) and Bartonella spp. 6/30 (20%) were detected in the screened cats. No sample was positive for genes dsb and 16S rRNA of Ehrlichia spp. Only those animals which were positive for R. vitalii showed findings compatible with rangeliosis, such as anemia (100%), thrombocytopenia (67%), jaundice (50%), external bleeding (50%), and anorexia (50%). This is the first time that B. vogeli detected among cats in Southern Brazil.

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“…IFAT antibody for E. canis was found in 17% of the dogs analyzed in this study, a similar seropositivity has been previously observed by Costa et al (2015) that found 14.6% on dogs from rural and urban areas of Maranhão, also a state located in the northeast Brazil. Other highest values of seropositivity in dogs were reported in Northeastern Brazil: Paraiba 72.…”

Section: Discussionsupporting

confidence: 91%

<b>Epidemiological aspects of tick-borne diseases in wild and domestic animals of two environmental protection areas in the city of Natal, Rio Grande do Norte State, Brazil</b>

Lopes¹

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DEDICATÓRIADedico a minha mãe Vitória Cedomia Ribeiro Gomes Lopes em cumprimento a nossa promessa. Há 23 anos, numa manhã de inverno, em uma rua calçada de pedras e cercada por casas simples à esquerda e terrenos de capim verde à direita, ela segurava minha mão me levando a escola quando eu olhei em seus olhos e pedi que ela me ajudasse a chegar até aqui. Nós sequer sabíamos onde era, mas ela prometeu. Ela nunca fraquejou em manter sua promessa apesar de eu tê-lo feito em vários momentos. Obrigado Vitória! "Sou como a haste fina: qualquer brisa verga, mas nenhuma espada corta! Pensou que eu ando só? " Maria Bethânia: Carta de amor ABSTRACT LOPES, M. G. Epidemiological aspects of tick-borne diseases in wild and domestic animals of two environmental protection areas in the city of Natal, Rio Grande do Norte State, Brazil. [Aspectos epidemiológicos de agentes transmitidos por carrapatos em animais silvestres e domésticos de duas Unidades de Conservação, na Cidade de Natal, RN]. 2016. 74 f. Tese (Doutorado em Ciências) -Faculdade de Medicina Veterinária e Zootecnia, Universidade de São Paulo, São Paulo, 2016. The aim of this study was to determine the serologic and molecular occurrence of Rickettsia spp., Ehrlichia spp., Babesia spp. and Hepatozoon spp. in ticks and domestic and wild animals from two conservation units in the city of Natal, Rio Grande do Norte State, Brazil. The collection period was between October 2012 and August 2013. Serum samples were tested against Rickettsia spp. antigens and Ehrlichia canis by Indirect fluorescent antibody test. Tissue samples and ticks were processed for molecular detection of the pathogens. Twenty-seven marsupials and four rodents were captured, and up to three animals of each species were euthanized. In addition, serum samples from 155 domestic animals: 53 cats living inside the units, 29 dogs domiciled around the areas and 73 dgos of the Zoonosis Control Center of the City (ZCC). Twenty dogs from ZCC were also euthanized and samples of spleen were obtained. Antibodies to at least one of the Rickettsia species tested were detected in six Didelphis albiventris and in one Rattus rattus; 17% (17/102) of the dogs presented antibodies to E. canis and 13% (20/155) of all tested domestic animals (dogs and cats) were seropositive for Rickettsia spp. antigens. Three species of ticks (Amblyomma auricularium, Ixodes loricatus and Ornithodoros mimon) were collected and one A. auricularium was positive for Rickettsia amblyommii by PCR. Two D. albiventris spleen samples amplified PCR products for Ehrlichia spp. Spleen samples from three D. albiventris and spleen and lung sample from one Necromys lasiurus were positive for Babesia spp. by PCR test. Among the 20 spleen samples from dogs subjected to molecular analysis, eight were positive by PCR for E. canis and two for H. canis.

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A serological and molecular survey of Babesia vogeli, Ehrlichia canis and Rickettsia spp. among dogs in the state of Maranhão, northeastern Brazil

Cited by 27 publications

References 58 publications

Prevalence of Babesia species in hunting dogs in Ogun State South West Nigeria

Prevalence of Babesia species in hunting dogs in Ogun State South West Nigeria

Identification of vector-borne pathogens in dogs and cats from Southern Brazil

<b>Epidemiological aspects of tick-borne diseases in wild and domestic animals of two environmental protection areas in the city of Natal, Rio Grande do Norte State, Brazil</b>

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