Assessment of phylogenetic diversity of bacterial microflora in drinking water using serial analysis of ribosomal sequence tags

Poitelon, Jean-Baptiste; Joyeux, Marc; Welté, B.; Duguet, J.P.; Prestel, Eric; Lespinet, Olivier; DuBow, Michael S.

doi:10.1016/j.watres.2009.07.020

Cited by 55 publications

(39 citation statements)

References 45 publications

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“…For the bulk water, our rank abundance data suggest that there is a wide variety of low-abundance phylotypes, which were at or below our detection limit of 0.1% relative abundance. This is consistent with other studies of pelagic bacterial communities, including drinking water (6,11,34,36). In contrast to the bulk water community, each biofilm community used for these calculations might consist, as demonstrated, of a unique set of bacterial species.…”

Section: Discussionsupporting

confidence: 89%

Analysis of Structure and Composition of Bacterial Core Communities in Mature Drinking Water Biofilms and Bulk Water of a Citywide Network in Germany

Henne

Kahlisch

Brettar

et al. 2012

Appl Environ Microbiol

133

123

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ABSTRACTThe bacterial core communities of bulk water and corresponding biofilms of a more than 20-year-old drinking water network were compared using 16S rRNA single-strand confirmation polymorphism (SSCP) fingerprints based on extracted DNA and RNA. The structure and composition of the bacterial core community in the bulk water was highly similar (>70%) across the city of Braunschweig, Germany, whereas all biofilm samples contained a unique community with no overlapping phylotypes from bulk water. Biofilm samples consisted mainly ofAlphaproteobacteria(26% of all phylotypes),Gammaproteobacteria(11%), candidate division TM6 (11%),Chlamydiales(9%), andBetaproteobacteria(9%). The bulk water community consisted primarily ofBacteroidetes(25%),Betaproteobacteria(20%),Actinobacteria(16%), andAlphaproteobacteria(11%). All biofilm communities showed higher relative abundances of single phylotypes and a reduced richness compared to bulk water. Only biofilm communities sampled at nearby sampling points showed similar communities irrespective of support materials. In all of our bulk water studies, the community composition determined from 16S rRNA was completely different from the 16S rRNA gene-based community composition, whereas in biofilms both molecular fractions resulted in community compositions that were similar to each other. We hypothesize that a higher fraction of active bacterial phylotypes and a better protection from oxidative stress in drinking water biofilms are responsible for this higher similarity.

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Section: Discussionsupporting

confidence: 89%

Analysis of Structure and Composition of Bacterial Core Communities in Mature Drinking Water Biofilms and Bulk Water of a Citywide Network in Germany

Henne

Kahlisch

Brettar

et al. 2012

Appl Environ Microbiol

133

123

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“…The increased numbers made it possible to detect more microorganisms (i.e., 1,133-1,731 OTUs at a 3% cutoff). These numbers are much greater than those in previous studies based on clone libraries in which generally less than 100 OTUs were identified in samples from freshwater (6,36) and drinking water treatment systems (6,17,21,33). The numbers of OTUs observed in this study were even higher than those from biofilm in two water meters investigated by pyrosequencing (133 and 208 OTUs) (11).…”

Section: Bacterial Diversitycontrasting

confidence: 78%

Pyrosequencing Demonstrated Complex Microbial Communities in a Membrane Filtration System for a Drinking Water Treatment Plant

et al. 2011

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Microbial community composition in a pilot-scale microfiltration plant for drinking water treatment was investigated using high-throughput pyrosequencing technology. Sequences of 16S rRNA gene fragments were recovered from raw water, membrane tank particulate matter, and membrane biofilm, and used for taxonomic assignments, estimations of diversity, and the identification of potential pathogens. Greater bacterial diversity was observed in each sample (1,133-1,731 operational taxonomic units) than studies using conventional methods, primarily due to the large number (8,275) of sequences available for analysis and the identification of rare species. Betaproteobacteria predominated in the raw water (61.1%), while Alphaproteobacteria were predominant in the membrane tank particulate matter (42.4%) and membrane biofilm (32.8%). The bacterial community structure clearly differed for each sample at both the genus and species levels, suggesting that different environmental and growth conditions were generated during membrane filtration. Moreover, signatures of potential pathogens including Legionella, Pseudomonas, Aeromonas, and Chromobacterium were identified, and the proportions of Legionella and Chromobacterium were elevated in the membrane tank particulate matter, suggesting a potential threat to drinking water treated by membrane filtration.

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“…482-740 OTUs at a 3% cut-off). These numbers are much greater than those based on clone libraries as reported in previous studies, of which less than 100 OTUs were identified from freshwater samples (Zwart et al 2002;Eichler et al 2006) and drinking water treatment systems (Martiny et al 2005;Eichler et al 2006;Poitelon et al 2009). The observed species in this study (184.4-507.1) were even higher than those observed by Navarro-Noya et al (2013) …”

Section: Species Richnessmentioning

confidence: 57%

“…Currently, the technique has been used to explore human microbiomes (Costello et al 2009) and environmental samples such as soil (Roesch et al 2007) and oceans (Sogin et al 2006). A few investigations have been performed on environmental water samples from either waste or drinking water (Cottrell et al 2005;Martiny et al 2005;Poitelon et al 2009). The aim of this study was to assess microbial communities in RHRW using samples from a village with a typical rural setting that includes rearing of a variety of domestic animals.…”

Section: Introductionmentioning

confidence: 99%

Pyrosequencing analysis of roof-harvested rainwater and river water used for domestic purposes in Luthengele village in the Eastern Cape Province of South Africa

Chidamba

Korsten

2015

Environ Monit Assess

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Pyrosequencing targeting the V1-V3 hypervariable of the 16S rDNA was used to investigate the bacterial diversity in river and roof-harvested rainwater (RHRW) used for potable purposes by rural households in Luthengele village in the Eastern Cape Province of South Africa. The phylum Proteobacteria dominated the data set (80.5% of all reads), while 4.2% of the reads could not be classified to any of the known phyla at a probability of 0.8 or higher (unclassified bacteria). At class level, the classes; Betaproteobacteria (50.4% of all reads), Alphaproteobacteria (16.2%), Verrucomicrobiae (6.6%), Planctomycetacia (5.7%) and Sphingobacteria (3%) dominated the data set in all the samples. Although the class Verrucomicrobiae constituted 6.6% of all sequences, 88.6% of the sequences were from the river sample where the class represented 43.7% of the observed sequences in the sample. The bacteria community structure clearly showed significant similarities between RHRW and differences with the river water control sample, suggesting different levels of contamination and environmental factors affecting the various water sources. Moreover, signatures of potential pathogens including Legionella, Acinetobacter, Pseudomonas, Clostridia, Chromobacterium, Yersinia and Serratia were detected, and the proportions of Legionella were relatively higher suggesting a potential health risk to households using RHRW. This work provides guidance for prioritizing subsequent culturable and quantitative analysis, to ensure that potentially significant pathogens are not left out of risk estimations.

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Assessment of phylogenetic diversity of bacterial microflora in drinking water using serial analysis of ribosomal sequence tags

Cited by 55 publications

References 45 publications

Analysis of Structure and Composition of Bacterial Core Communities in Mature Drinking Water Biofilms and Bulk Water of a Citywide Network in Germany

Analysis of Structure and Composition of Bacterial Core Communities in Mature Drinking Water Biofilms and Bulk Water of a Citywide Network in Germany

Pyrosequencing Demonstrated Complex Microbial Communities in a Membrane Filtration System for a Drinking Water Treatment Plant

Pyrosequencing analysis of roof-harvested rainwater and river water used for domestic purposes in Luthengele village in the Eastern Cape Province of South Africa

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