Pyrosequencing Demonstrated Complex Microbial Communities in a Membrane Filtration System for a Drinking Water Treatment Plant

Kwon, Soondong; Moon, Eunjeong; Kim, Taek Seung; Hong, Seungkwan; Park, Hee Deung

doi:10.1264/jsme2.me10205

Cited by 75 publications

(50 citation statements)

References 31 publications

Supporting

Mentioning

Contrasting

Order By: Relevance

“…The absence (or their presence at abundances lower than detection limits) of Aeromonas and Enterobacter cells on filter material agrees with previous studies showing poor survival of those bacteria in drinking water biofilm (Kwon et al, 2011;Lehtola et al, 2007). The filtration process is confirmed as an effective treatment for ensuring a two-log reduction of faecal indicators from the influent water over 27 cm total filter depth, regardless of the material employed.…”

Section: Implications For Water Quality: Putative Pathogens and Faecasupporting

confidence: 89%

Medium shapes the microbial community of water filters with implications for effluent quality

et al. 2018

View full text Add to dashboard Cite

a b s t r a c tLittle is known about the forces that determine the assembly of diverse bacterial communities inhabiting drinking water treatment filters and how this affects drinking water quality. Two contrasting ecological theories can help to understand how natural microbial communities assemble; niche theory and neutral theory, where environmental deterministic factors or stochastic factors predominate respectively. This study investigates the development of the microbial community on two common contrasting filter materials (quartz sand and granular activated carbon-GAC), to elucidate the main factors governing their assembly, through the evaluation of environmental (i.e. filter medium type) and stochastic forces (random deaths, births and immigration). Laboratory-scale filter columns were used to mimic a rapid gravity filter; the microbiome of the filter materials, and of the filter influent and effluent, was characterised using next generation 16S rRNA gene amplicon sequencing and flow-cytometry. Chemical parameters (i.e. dissolved organic carbon, trihalomethanes formation) were also monitored to assess the final effluent quality. The filter communities seemed to be strongly assembled by selection rather than neutral processes, with only 28% of those OTUs shared with the source water detected on the filter medium following predictions using a neutral community model. GAC hosted a phylogenetically more diverse community than sand. The two filter media communities seeded the effluent water, triggering differences in both water quality and community composition of the effluents. Overall, GAC proved to be better than sand in controlling microbial growth, by promoting higher bacterial decay rates and hosting less bacterial cells, and showed better performance for putative pathogen control by leaking less Legionella cells into the effluent water.

show abstract

Section: Implications For Water Quality: Putative Pathogens and Faecasupporting

confidence: 89%

Medium shapes the microbial community of water filters with implications for effluent quality

et al. 2018

View full text Add to dashboard Cite

show abstract

“…These organisms have been linked to many types of diseases, and there have been numerous reports of infection by these organisms in humans, some of which have led to fatalities (Baldi et al 2010;Durán et al 2010). Chromobacterium species were present in a pilot-scale microfiltration plant for drinking water treatment (Kwon et al 2011). However, to the authors' knowledge, this is the first report concerning the existence of genus Chromobacterium in the drinking water filter.…”

Section: Potential Microbiological Risksmentioning

confidence: 85%

Characterization of microbial communities in a granular activated carbon–sand dual media filter for drinking water treatment

Feng

Chen

Wang

et al. 2013

Int. J. Environ. Sci. Technol.

View full text Add to dashboard Cite

Modification of conventional rapid sand filter into granular activated carbon-sand dual media filter has become increasingly attractive to drinking water producers in many Chinese urban regions. In this study, a pilot-scale dual media filter was constructed to monitor its performance. The operational results indicated that the dual media filter performed well both on nitrification and on reduction of chemical oxygen demand. Microbial community structures at different sampling sites were also compared using 16S ribosomal ribonucleic acid gene clone library analysis to identify the spatial heterogeneity the filter. Clone library analysis illustrated the difference of microbial community structure and an increase of microbial diversity along the filter depth. Moreover, potential pathogens from genera Chromobacterium and Sphingomonas were detected in the filter samples, which deserved further serious consideration in downstream treatment such as disinfection. These findings also provided an illustration of the utility of molecular techniques to discover microbial community change and potential pathogens in the drinking water biofilters.

show abstract

“…Several studies have used high-throughput sequencing technologies to investigate bacterial community diversity in water distribution systems locations or components (Kwon, 2011;Pinto et al, 2012;Douterelo et al, 2013;Navarro-Noya et al, 2013). In a recent study, we highlight the importance for such investigations in First Nations communities in Canada (Fernando et al, 2016).…”

Section: Introductionmentioning

confidence: 94%

Bacteria in drinking water sources of a First Nation reserve in Canada

Farenhorst

Jahan

et al. 2017

Science of The Total Environment

View full text Add to dashboard Cite

Approximately 20% of the 600 First Nations reserves across Canada are under a drinking water advisory, often due to unacceptable levels of bacteria. In this study, we detected fecal bacteria at an alarmingly high frequency in drinking water sources in a fly-in First Nations community, most notably in buckets/drums of homes without running water where Escherichia coli levels ranged from 20 to 62,000 CFU/100mL. The water leaving the water treatment plant was free of E. coli and its free residual chlorine concentration (0.67 mg/L) was within the range typically observed for treated water in Canada. Water samples from taps in homes served by cisterns, and those sampled from the water truck and community standpipe, always showed unacceptable levels of E. coli (1 to 2,100 CFU/100mL) and free residual chlorine concentrations below the 0.2 mg/L required to prevent bacterial regrowth. Samples from taps in homes served by piped water had lower levels of E. coli (0 to 2 CFU/100mL). DNA-and RNA-based 16S rRNA Illumina sequencing demonstrated that piped and cisterns water distribution systems showed an abundance of viable cells of Alphaproteobacteria indicative of biofilm formation in pipes and cisterns. The alpha diversity, based on observed OTUs and three other indices, was lowest in water truck samples that supplied water to the cistern and the low free residual chlorine concentration (0.07 mg/L) and predominance of Betaproteobacteria (63% of viable cells) that were immediately detected after the truck had filled up at the water treatment plant was indicative of contamination by particulate matter. Given these findings, First Nation residents living without running water and relying on inadequate water distribution systems are at higher risk of contracting water-born illnesses. We urge all governments in Canada to expand their investments in supporting and sustaining water as a human right in Canada's First Nations communities. 3 GRAPHICAL ABSTRACT

show abstract

Pyrosequencing Demonstrated Complex Microbial Communities in a Membrane Filtration System for a Drinking Water Treatment Plant

Cited by 75 publications

References 31 publications

Medium shapes the microbial community of water filters with implications for effluent quality

Medium shapes the microbial community of water filters with implications for effluent quality

Characterization of microbial communities in a granular activated carbon–sand dual media filter for drinking water treatment

Bacteria in drinking water sources of a First Nation reserve in Canada

Contact Info

Product

Resources

About