Assessment of Micronucleus Frequency in Normal Oral Mucosa of Patients Exposed to Carcinogens

Bohrer, Paula Luce; Filho, Manoel Sant’Ana; Paiva, R L; Silva, Isabel Lauxen da; Rados, Pantelis Varvaki

doi:10.1159/000326148

Cited by 38 publications

(36 citation statements)

References 24 publications

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“…Also, the effect of age on micronuclei formation in buccal cells is unclear. In five investigations, no effect was detected (6,13,27,29,30); in two, an increase with age (39,40) and in another a decrease with age (25) were detected. Piyathilake et al (40) reported that the frequencies of micronucleated cells are 2.8-fold higher in females after adjusting for race, age, smoking habit, and levels of selected vitamin, whereas in a Brazilian study (27) the number of micronuclei was significantly (i.e., >3-fold) higher in males.…”

Section: Discussionmentioning

confidence: 86%

“…Although some investigators (6,13,26,27,30) found no effect of alcohol on micronuclei formation, Gabriel et al (31) showed a significant effect. Also, Stich and Rosin (26) reported on a pronounced synergistic effect of alcohol in combination with smoking, whereas in another study (25) even an inverse relation was found.…”

Section: Discussionmentioning

confidence: 97%

“…Because our findings indicate that micronuclei studies with nonspecific stains reflect the levels of keratinization of the cells, it can be expected that strong site-specific effects are found when cells are collected from different areas as it is known the levels of karyorrhexis and keratinization in the oral cavity differ strongly. They are highest in the lips, whereas only low levels are found in the bottom of the mouth (13). Currently, Giemsa stains are also widely used in micronuclei studies with nonepithelial cells originating from a number of organs, e.g., in experiments with polychromatic erythrocytes in bone marrow of rodents (44), lymphocytes (45), splenocytes (46), and liver cells (47), and in in vitro studies with stable cell lines (48).…”

Section: Discussionmentioning

confidence: 99%

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Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

Nersesyan

Kundi

Atefie

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

167

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Micronuclei in exfoliated epithelial cells are widely used as biomarkers of cancer risk in humans. To elucidate the effect of different staining procedures on the outcome of such investigation, we conducted a study in which the micronuclei frequencies in oral mucosa cells of heavy smokers (n = 20) and nonsmokers (n = 10) were evaluated with nonspecific (Giemsa, May-Grü nwald-Giemsa) and DNA-specific (4 ¶,6-diamidino-2-phenylindole, Feulgen, acridine orange) stains, whereas with Giemsa-based stains, the frequencies of micronuclei in smokers were significantly (4-to 5-fold) higher in the smokers group, no significant increase was observed with any of the DNA-specific stains. Furthermore, the evaluation of cells of the two study groups with Feulgen stain showed that oral mucosa cells from smokers had significantly increased levels of nuclear anomalies other than micronuclei.These anomalies are consequences of cell injury found in epithelial cells and are paralleled by formation of keratin bodies in the cytoplasm that resemble micronuclei. Correlation analyses showed that micronuclei frequencies scored in Giemsa-stained slides correlated significantly with karyorrhexis, karyolysis, condensed chromatin, and binucleates, whereas no such correlations were found with DNA-specific stains. These findings indicate that nuclear anomalies (and possibly keratin bodies) may be misinterpreted as micronuclei with nonspecific DNA stains and lead to false-positive results in studies with cells of epithelial origin. Furthermore, our results show that exposure of oral mucosa cells to genotoxic carcinogens contained in tobacco smoke does not lead to induction of micronuclei in these cells. (Cancer Epidemiol Biomarkers Prev 2006;15(10):1835 -40)

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Section: Discussionmentioning

confidence: 86%

Section: Discussionmentioning

confidence: 97%

Section: Discussionmentioning

confidence: 99%

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Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

Nersesyan

Kundi

Atefie

et al. 2006

Cancer Epidemiology, Biomarkers &Amp; Prevention

167

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“…Este resultado sugere que esta estrutura não está relacionada à genotoxicidade. A ausência de associação positiva entre broken eggs e insulto genotóxico também foi relatada por diversos autores (BOHRER et al, 2005;CERQUEIRA et al, 2008;HOLLAND et al, 2008;TORRES-BUGARÍN et al, 2003). Segundo Holland et al (2008) broken eggs está relacionado com amplificação gênica e não representa danos ao DNA.…”

Section: Discussionunclassified

“…Segundo Holland et al (2008) broken eggs está relacionado com amplificação gênica e não representa danos ao DNA. Torres-Bugarín et al (2003) e Bohrer et al (2005) corroboram esta hipótese e salientam que estas estruturas estão associadas ao processo normal de diferenciação celular.…”

Section: Discussionunclassified

Danos cromossômicos e alterações nucleares em células esfoliadas do epitélio gengival de indivíduos com periodontite crônica moderada

Silva

Neris²,

Lopes

et al. 2017

cmbio

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ResumoIntrodução: a periodontite é uma doença multifatorial que resulta primariamente da ação de bactérias nos tecidos que compõem o periodonto. O processo inflamatório que se desenvolve em consequência está associado ao aumento da proliferação celular que propicia a ocorrência de danos no material genético. Objetivo: avaliar, com uso do teste de micronúcleo, a ocorrência de danos genéticos e alterações nucleares em indivíduos com periodontite crônica moderada. Metodologia: a amostra incluiu dez indivíduos portadores de periodontite crônica moderada em início do tratamento no Centro de Especialidades Odontológicas (CEO) de Senhor do Bonfim/BA. Os participantes responderam a um questionário e células esfoliadas da mucosa gengival de regiões sem alterações e com periodontite crônica moderada foram coletadas. Este material foi transferido para lâmina de vidro, fixado com metanol/ácido acético (3:1), corado com reativo de Shiff e fast green e analisado sob microscopia óptica. A análise estatística foi feita com o teste condicional para comparação de proporções em situações de eventos raros. Resultados: a ocorrência de micronúcleos, alterações nucleares degenerativas (cariorréxis, cariólise, cromatina condensada, picnose) e vacúolos nucleares foi significativamente maior em células da lesão. A ocorrência de broken eggs não diferiu estatísticamente. Conclusões: a periodontite crônica moderada é uma doença na qual há comprometimento da integridade genética das células do epitélio gengival. A maior ocorrência de alterações indicativas de apoptose na área da periodontite crônica aponta para a resposta biológica do organismo ao insulto genotóxico ao qual as células nesta lesão estão submetidas. Palavras-chave: Periodontite. Genotoxicidade. Apoptose. Abstract Introduction: periodontitis is a multifactorial disease that results primarily from the action of bacteria in the tissues that compose the periodontium. The inflammatory process that develops as a consequence is associated with increased cell proliferation which facilitates

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The use of cytopathology to identify disturbances in oral squamous cell carcinoma at early stage: A case report

Daroit

Salgueiro

Maito

et al. 2018

Diagnostic Cytopathology

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Early detection of oral squamous cell carcinoma (OSCC) is a challenge for oral surgeons, as clinical features are not always classical. Cytopathological assays can help identify alterations at the cellular level. This article reports a case of OSCC in a young male adult without exposure to classical risk factors. The histopathological examination showed a micro invasive carcinoma invading the connective tissue. Cytopathological results showed a higher percentage of cells in deeper epithelial layers; the cytomorphometric examination revealed a nuclear/cytoplasmic ratio of 0.14; the mean number of Nucleolar Organiser Regions which have a high affinity for silver (AgNOR) per nucleus was 2.86, and the mean percentage of nuclei with >2 AgNOR was 58%. The micronucleus test found 3 micronucleated cells and several metanuclear aberrations. These findings support the hypothesis that cytological examination is an important tool to identify early changes in oral smears and thus help in the early clinical detection of suspicious malignant oral lesions that should be more rigorously followed.

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Assessment of Micronucleus Frequency in Normal Oral Mucosa of Patients Exposed to Carcinogens

Cited by 38 publications

References 24 publications

Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

Effect of Staining Procedures on the Results of Micronucleus Assays with Exfoliated Oral Mucosa Cells

Danos cromossômicos e alterações nucleares em células esfoliadas do epitélio gengival de indivíduos com periodontite crônica moderada

The use of cytopathology to identify disturbances in oral squamous cell carcinoma at early stage: A case report

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