Assessment of IL‐18 Serum Level and Its Promoter Polymorphisms in the Saudi Coronary Artery Disease (CAD) Patients

Jabir, Nasimudeen R.; Firoz, Chelapram K.; Kamal, Mohammad Amjad; Damanhouri, Ghazi A.; Alama, Mohammed Nabil; Alam, Qamre; Haque, Absarul; Almehdar, Hussein A.; Tabrez, Shams

doi:10.1002/jcb.25870

Cited by 14 publications

(4 citation statements)

References 27 publications

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“…In our earlier studies, we reported non‐significant associations of SNPs in promoter region of IL‐6, IL‐18, and IL‐1β genes with Saudi CVD patients . However, the present study reports a significant association between polymorphism in IL‐10 promoters at −1082 and −819 sites with Saudi CVD patients.…”

Section: Discussioncontrasting

confidence: 78%

“…In our earlier studies, we have reported a significant elevation in different inflammatory mediators such as IL‐6, IL‐18, and IL‐1β in Saudi CVD patients . In this study too, we observed a significant elevation in IL‐10 level in CVD patients and other cardiac events (Table ).…”

Section: Discussionsupporting

confidence: 68%

“…The purification of PCR products were carried out by using PCR purification kit (Thermo Scientific, USA). Thermal cycling and Sanger sequencing were performed as described earlier by us . Because of the long nucleotide stretch, one internal primer (5′ TGGAGATGGTGTACA 3′) was also used to read the whole coding region in the control and CVD samples.…”

Section: Methodsmentioning

confidence: 99%

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A putative association of interleukin‐10 promoter polymorphisms with cardiovascular disease

et al. 2017

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Interleukin-10 (IL-10) is an anti inflammatory cytokine involved in the ongoing coronary inflammation and related patho-physiological processes. The piece of work presented herein is aimed at investigating possible association of polymorphisms in IL-10 promoter with Saudi cardiovascular disease (CVD) patients. The study included 80 confirmed CVD patients with diabetes and 75 healthy control individuals both men and women. Concentration of IL-10 in the serum samples were measured by ELISA method. For single nucleotide polymorphism (SNP) analysis, Sanger method of DNA sequencing was followed. The IL-10 level was found to be significantly elevated in CVD patients (P < 0.001) and its associated complications viz. ST-elevation myocardial infarction [STEMI] (P <0.01), non ST-elevation myocardial infarction [NSTEMI] (P < 0.05), and unstable angina [UA] (P < 0.001). We also observed a significant association between polymorphisms in IL-10 promoter at -1082 and -819 locus with Saudi CVD patients. Moreover, at -1082 A/G locus, AA haplotype was found to be less frequent in the CVD patients compared with control individuals. On the other hand, highly significant rise in heterozygous (A/G genotype) condition was observed in patient samples compared with control ones (P < 0.001). Similarly, the genotypic frequencies at -819 C/T locus were also found to be significantly associated (P < 0.001) with CVD patients compared with control individuals. Our study provides the status of polymorphism in IL-10 promoter and its association with CVD risk in Saudi population. As per our information, ours is the first article that shows the genetic diversity in IL-10 promoters and its level in the Saudi CVD patients. © 2017 IUBMB Life, 69(7):522-527, 2017.

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Section: Discussioncontrasting

confidence: 78%

Section: Discussionsupporting

confidence: 68%

Section: Methodsmentioning

confidence: 99%

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A putative association of interleukin‐10 promoter polymorphisms with cardiovascular disease

et al. 2017

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“…The purification of PCR products was carried out by using PCR purification kit (Thermo Scientific). The subsequent positive DNA clones were sequenced and analyzed as described in Jabir et al [2016aJabir et al [ , 2017.…”

Section: Dna Sequencing and Analysismentioning

confidence: 99%

Estimation of Interleukin-1β Promoter (−31 C/T and −511 T/C) Polymorphisms and Its Level in Coronary Artery Disease Patients

et al. 2017

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Interleukin-1β (IL-1β) is an inflammation-causing cytokine that exerts several unique biological effects and could lead to future adverse events of CAD. The piece of work presented herein is aimed at investigating possible association of IL-1β levels to its polymorphic site viz. -511 and -31 at promoter region in Saudi CAD patients. The study included 155 confirmed CAD patients and 80 healthy control individuals both men and women. Concentration of IL-1β in the patients' serum was measured by ELISA method. For single nucleotide polymorphism (SNP) analysis, sanger method of DNA sequencing was followed. We observed variable numbers of SNPs at -31 C/T and -511 T/C promoter regions in Saudi patients suffering from CAD in comparison to the control set of individuals. However, the changes in the number of SNP-hotspots were determined to be non-significant with reference to the control set. The haplotype analysis at -31 and -511 also did not show any significant changes between control and CAD patients. Moreover, serum IL-1β levels were observed to be expressively higher in patients suffering from CAD (P < 0.001) and its associated complications viz. STEMI (P < 0.001), NSTEMI (P < 0.001), and UA (P < 0.001). Our study provides the status of SNPs at IL-1β promoter in Saudi population. As per our information, ours is the first article that shows the genetic diversity in IL-1β promoters and its level in the Saudi CAD patients. J. Cell. Biochem. 118: 2977-2982, 2017. © 2017 Wiley Periodicals, Inc.

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Interleukin-18 promoter −137 G/C polymorphism (rs187238) is associated with biochemical markers of renal function and cardiovascular disease in type 2 diabetes patients

Cavalcante

Sousa

Rodrigues

et al. 2020

Clinical Biochemistry

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Assessment of IL‐18 Serum Level and Its Promoter Polymorphisms in the Saudi Coronary Artery Disease (CAD) Patients

Cited by 14 publications

References 27 publications

A putative association of interleukin‐10 promoter polymorphisms with cardiovascular disease

A putative association of interleukin‐10 promoter polymorphisms with cardiovascular disease

Estimation of Interleukin-1β Promoter (−31 C/T and −511 T/C) Polymorphisms and Its Level in Coronary Artery Disease Patients

Interleukin-18 promoter −137 G/C polymorphism (rs187238) is associated with biochemical markers of renal function and cardiovascular disease in type 2 diabetes patients

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