Interleukin-10 (IL-10) is an anti inflammatory cytokine involved in the ongoing coronary inflammation and related patho-physiological processes. The piece of work presented herein is aimed at investigating possible association of polymorphisms in IL-10 promoter with Saudi cardiovascular disease (CVD) patients. The study included 80 confirmed CVD patients with diabetes and 75 healthy control individuals both men and women. Concentration of IL-10 in the serum samples were measured by ELISA method. For single nucleotide polymorphism (SNP) analysis, Sanger method of DNA sequencing was followed. The IL-10 level was found to be significantly elevated in CVD patients (P < 0.001) and its associated complications viz. ST-elevation myocardial infarction [STEMI] (P <0.01), non ST-elevation myocardial infarction [NSTEMI] (P < 0.05), and unstable angina [UA] (P < 0.001). We also observed a significant association between polymorphisms in IL-10 promoter at -1082 and -819 locus with Saudi CVD patients. Moreover, at -1082 A/G locus, AA haplotype was found to be less frequent in the CVD patients compared with control individuals. On the other hand, highly significant rise in heterozygous (A/G genotype) condition was observed in patient samples compared with control ones (P < 0.001). Similarly, the genotypic frequencies at -819 C/T locus were also found to be significantly associated (P < 0.001) with CVD patients compared with control individuals. Our study provides the status of polymorphism in IL-10 promoter and its association with CVD risk in Saudi population. As per our information, ours is the first article that shows the genetic diversity in IL-10 promoters and its level in the Saudi CVD patients. © 2017 IUBMB Life, 69(7):522-527, 2017.

The present study was carried on milk production and milk constituents of 444 lactations of 176 Murrah buffaloes over a period of 10 years from 2001 to 2010 with the objective to find out the effect of various genetic and non-genetic factors on milk yield and milk constituents traits in Murrah buffaloes. Data on total milk yield, milk yield in 305 days or less, lactational average fat per cent and lactational average solid not fat percent (LSA) were collected and compiled purposely and analyzed with suitable statistical tool. Overall least square mean for all lactation traits of total milk yield (TMY), milk yield in 305 days or less (305MY), lactational average fat % (LFA) and lactational average solid not fat % (LSA) were 2253.88 ± 70.15 Kg, 2097.11 ± 59.12 Kg, 7.97 ± 0.02 % and 9.64 ± 0.01 % respectively. The period has significant effect on milk yield and milk constituent traits. Sire and parity affects significantly to TMY and 305MY, however, seasons were affecting to TMY only. Hence it is concluded as milk constituents (LFA and LSA) not affected by sire, parity and season. Although, sire and parity has affect on TMY and 305MY whereas season affects only TMY, therefore, due importance should be given to sire and parity during selection and genetic improvement of Murrah buffalo. Analysis among the parities also revealed that there were no significant (P0.05) difference among the second, third and fourth parity hence, at the time of selection second lactation (305MY) milk yield is equally important as fourth lactation in which highest milk yield has been reported in present study.

The present study pertained to records on milk production and milk constituents of 259 Sahiwal cattle with 600 lactations spread over a period of 10 years from 2001 to 2010, collected from Dairy Cattle Breeding division of National Dairy Research Institute, Karnal, Haryana. To study the effect of various genetic and non-genetic factors on milk yield and milk constituents traits, mixed model least square analysis was used for analysis of data. Overall least square mean for all lactation traits of total milk yield (TMY), milk yield in 305 days or less (305MY), lactational average fat % (LFA) and lactational average solid not fat % (LSA) were 1880.39 ± 73.82 Kg, 1782.97 ± 68.37 Kg, 4.71 ± 0.01 % and 8.81 ± 0.01 % respectively. Analysis of variance showed that the differences were statistically significant for the effect of sire on TMY and 305MY; period on all the traits except TMY; parity on 305MY. However the effect of season of calving was not found significant on all the traits.

The present review highlights the various causatives of SLE, particularly the genetic alteration in B- and T-cell-related proteins. We have also delineated some of the available therapeutic strategies for the treatment of SLE.

:The present study pertained to records on milk production and milk constituents of 259 Sahiwal cattle with 600 lactations spread over a period of 10 years from 2001 to 2010, collected from Dairy Cattle Breeding division of National Dairy Research Institute, Karnal, Haryana. To study the effect of various genetic and non-genetic factors on milk yield and milk constituents traits, mixed model least square analysis was used for analysis of data. Overall least square mean for all lactation traits of total milk yield (TMY), milk yield in 305 days or less (305MY), lactational average fat per cent (LFA) and lactational average solid not fat per cent (LSA) were 1880.39 ± 73.82 kg, 1782.97 ± 68.37 kg, 4.71 ± 0.01 per cent and 8.81 ± 0.01 per cent, respectively. Analysis of variance showed that the differences were statistically significant for the effect of sire on TMY and 305MY; period on all the traits except TMY; parity on 305MY. However, the effect of season of calving was not found significant on all the traits.