Assessment of hepatoprotective potential of N. indicum leaf on haloalkane xenobiotic induced hepatic injury in Swiss albino mice

Dey, Priyankar; Dutta, S.; Sarkar, Mousumi Poddar; Chaudhuri, Tapas Kumar

doi:10.1016/j.cbi.2015.03.025

Cited by 22 publications

(18 citation statements)

References 41 publications

(51 reference statements)

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“…Our previous study showed potent iron chelation ability by the oleander extracts [11]. Recent studies also showed that 70 % hydro-methanolic extract of N. indicum leaf could ameliorate hemochromatosis and fibrosis in liver due to its potent antioxidant and iron chelation activities [13, 14]. Thus, the present study corroborates the previous findings of the antioxidative protection by oleander extracts.…”

Section: Discussionsupporting

confidence: 90%

“…N. indicum leaf extract has previously been shown to normalize similar liver biomarkers under CCl 4 toxicity [14]. Moreover, oleander floral extracts normalized the elevated AST, GGT, ALT, LDH, ALP and creatinine levels in blood under isoproterenol-induced oxidative stress in rats [34].…”

Section: Discussionmentioning

confidence: 99%

“…The in vitro hepatoprotective capacity of NOSE and NORE were studied according to previously standardized methods with minor modifications [14, 21, 22]. Briefly, seven groups of primary explant culture of mice hepatocytes were prepared in RPMI-1640 medium (containing 50 U/ml penicillin, 50 U/ml streptomycin and 50 U/ml nystatin) supplemented with 10 % FBS.…”

Section: Methodsmentioning

confidence: 99%

“…It is interesting to note that, a recent in vivo preliminary study demonstrated that N. indicum leaf extract ameliorates haemochromatic conditions in mouse [13]. Moreover, another recent study showed that N. indicum leaf extract attenuates CCl 4 mediated hepatotoxicity in Swiss albino mouse [14]. Simultaneously, the same 70 % hydro-methanolic extract of oleander leaf possess anti-inflammatory activities by modulation of Th1/Th2 cytokine balance, inhibition of cyclooxygenase, prostaglandin E 2 (PGE 2 ) and nitric oxide levels in mitogen induced splenic lymphocytes [15, 16].…”

Section: Introductionmentioning

confidence: 99%

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Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

Dey

Dutta

Biswas-Raha

et al. 2016

BMC Complement Altern Med

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BackgroundNerium oleander L. (syn. Nerium indicum Mill, Nerium odorum Aiton) belongs to the family Apocynaceae. It is used for its anti-inflammatory, anti-diabetic, anti-cancer and hepatoprotective activities in traditional medicine. Previous pharmacognostic studies suggested that 70 % hydro-methanolic extracts of oleander possess potent free radical scavenging and anti-inflammatory activities, both of which are helpful against hepatotoxicity.MethodsHydro-methanolic extracts of oleander stem and root were evaluated for their hepatoprotective activities in acute CCl4 intoxicated mouse through in vitro and in vivo studies. Silymarin was used as positive reference. Antioxidant enzymes, pro-inflammatory markers and liver enzymatic and biochemical parameters were studied. The extracts were further chemically characterized using Fourier Transform Infrared (FTIR) spectroscopy and Gas chromatography-mass spectrometry (GC-MS).ResultsCCl4 toxicity caused fatty liver formation by increase of relative liver weight (32.53 g) compared to control group (16.08 g). The elevated liver enzymatic and biochemical parameters due to CCl4 toxicity were considerably normalized by the extracts treatment under both in vivo and in vitro models. Oleander stem (NOSE) and root (NORE) extracts increased the reduced hepatic catalase activity 27.37 and 25.25 %, whereas peroxidase activity was increased 18.19 and 22.78 %, respectively. The extent of lipid peroxidation was significantly (p < 0.01) lowered 20.76 % (NOSE) and 21.12 % (NORE) compared to CCl4 group. The levels of pro-inflammatory tumor necrosis factor-α (TNF-α) was lowered 71.33 % (NOSE) and 61.60 % (NORE). Histopathological study demonstrated substantial reduction of hepatocellular necrosis, fatty infiltration, sinusoidal dilation, bile duct proliferation, vascular congestion, leukocyte infiltration in the silymarin and extract treated groups. Furthermore, various bioactive compounds were identified in the extracts such as apocynin, tocopherol, squalene, vanillin, isoeugenol, amyrin, lupeol etc.ConclusionThe present study provided convincing evidence that oleander extracts possess potent hepatoprotective capacity which was primarily governed by its antioxidant and anti-inflammatory activities. The collegial bioactivities of the phytochemicals may be accredited behind the hepatoprotective activity of oleander.Electronic supplementary materialThe online version of this article (doi:10.1186/s12906-016-1260-4) contains supplementary material, which is available to authorized users.

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Section: Discussionsupporting

confidence: 90%

Section: Discussionmentioning

confidence: 99%

Section: Methodsmentioning

confidence: 99%

Section: Introductionmentioning

confidence: 99%

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Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

Dey

Dutta

Biswas-Raha

et al. 2016

BMC Complement Altern Med

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“…The bio‐active compounds of the raspberry fruit extracts (RLE and RTE) were identified by GC‐MS analysis according to standard methods with slight modifications (Dey, Dutta, Sarkar, & Chaudhuri, ). Thermo Scientific Trace 1300 GC, equipped with ISQ‐QD (Thermo Scientific) single quadruple mass analyzer and TG‐5MS column (30 m × 0.25 mm × 0.25 μm) were employed for the identification of bio‐active compounds.…”

Section: Methodsmentioning

confidence: 99%

Wild raspberry: Antioxidant fruits from Eastern Himalaya

2018

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Raspberry fruits were used to investigate their possible activity against free radicals with possible implication in alleviating oxidative stress. A detailed in vitro antioxidant profiling, has been executed with a motive to evaluate the free radical scavenging activity of raspberry fruits extract. The immune cell proliferative properties (MTT) and in vitro assay for evaluation of their antioxidant activities including hydroxyl radical, nitric oxide, singlet oxygen, peroxynitrite and hydrogen peroxide were investigated. GC‐MS and FTIR analysis have been performed for the proper identification of active biological compounds. The results showed that raspberry fruit extracts have potent free radical scavenging capacity. ROS generated due to H2O2 in HEK‐293 cell line were normalized in the highest group (200 µg/mL) when compared with control group. MTT assay also proved that raspberry fruit extracts have the potentiality to modulate the immune system. In the present study, the peak characteristics of FTIR revealed that raspberry fruit extracts might be a probable source of many natural phyto‐compounds. The compounds identified by the GC‐MS analysis are known to have potent antioxidant activity. The present study clearly demonstrates that raspberry fruit extracts (Rubus lineatus and Rubus treutleri) are effective in alleviating ROS and RNS related complexities while being absolutely safe for human consumption and beneficial for the immune system. Practical applications Naturally occurring antioxidants have become increasingly popular over its synthetic counterpart in food, pharmaceutical and even in cosmetic industries due to its minimal side effects and ease in absorbance in physiological environment namely human body. Local people of Darjeeling Hills consume raspberry unaware of its medicinal properties. The aim of our study is to establish the medicinal properties of raspberry fruits through the antioxidant and cytotoxic activity.

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Antiinflammatory phytochemicals against virus‐induced hyperinflammatory responses: Scope, rationale, application, and limitations

Baranwal

Gupta

Dey

et al. 2021

Phytotherapy Research

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Uncontrolled inflammatory responses or cytokine storm associated with viral infections results in deleterious consequences such as vascular leakage, severe hemorrhage, shock, immune paralysis, multi‐organ failure, and even death. With the emerging new viral infections and lack of effective prophylactic vaccines, evidence‐based complementary strategies that limit viral infection‐mediated hyperinflammatory responses could be a promising approach to limit host tissue injury. The present review emphasizes the potentials of antiinflammatory phytochemicals in limiting hyperinflammatory injury caused by viral infections. The predominant phytochemicals along with their mechanism in limiting hyperimmune and pro‐inflammatory responses under viral infection have been reviewed comprehensively. How certain phytochemicals can be effective in limiting hyper‐inflammatory response indirectly by favorably modulating gut microbiota and maintaining a functional intestinal barrier has also been presented. Finally, we have discussed improved systemic bioavailability of phytochemicals, efficient delivery strategies, and safety measures for effective antiinflammatory phytotherapies, in addition to emphasizing the requirement of tightly controlled clinical studies to establish the antiinflammatory efficacy of the phytochemicals. Collectively, the review provides a scooping overview on the potentials of bioactive phytochemicals to mitigate pro‐inflammatory injury associated with viral infections.

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Assessment of hepatoprotective potential of N. indicum leaf on haloalkane xenobiotic induced hepatic injury in Swiss albino mice

Cited by 22 publications

References 41 publications

Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

Haloalkane induced hepatic insult in murine model: amelioration by Oleander through antioxidant and anti-inflammatory activities, an in vitro and in vivo study

Wild raspberry: Antioxidant fruits from Eastern Himalaya

Antiinflammatory phytochemicals against virus‐induced hyperinflammatory responses: Scope, rationale, application, and limitations

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