Assessment of hemolytic effect of Cassia flower extracts on human RBCs

Phuse, Shital S.; Khan, Zia H

doi:10.22270/jddt.v8i6-s.2169

Cited by 3 publications

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“…The LC-TOF MS was carried out for further identification and characterization of isolated compounds by PLC and the isolated luteolin LC/MS chromatogram and ion mass fragmentation spectra are depicted in Figure (6). From the full scan mass spectra of the isolated luteolin, the [M+H] + ion with m/z was 287.05, was selected as a molecular ion peak, and the most abundant fragments were 252 m/z (C15H8O4 + ), 133 m/z(C8H5O2 + ), 109 m/z(C6H4O2 + ).…”

Section: Lc-ms/ms-q-tof Resultsmentioning

confidence: 99%

“…The amount of isolated proposed luteolin from H3 fraction and the percent of isolated proposed chlorogenic acid from H4 fraction are listed in Table (6). Table 6.The amount in (µg/g) of isolated proposed luteolin and the percent of the isolated proposed chlorogenic acid.…”

Section: Figure 5 Calibration Curve Of Proposed Luteolin By Hplcmentioning

confidence: 99%

“…The seeds oil is used in the indigenous system of medicine for the treatment of skin diseases and leucoderma (5) . In the case of traditional Brazilian medicine, it has been used for the treatment of flu, cold, fever, and headache (6) . while the decoction of the roots is commonly used to treat snake bites (7) .…”

Section: Introductionmentioning

confidence: 99%

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Isolation, Identification, and Quantification of Two Compounds from Cassia glauca Cultivated in Iraq

Kanaan M Abdlkareem,

Kadhim

2023

IJPS

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The Cassia glauca Lam. is the tree that belongs to the Fabaceae family and is native to India has many uses in indigenous systems of medicine, folk medicine, and traditional Brazilian medicine. Has many pharmacological activities such as anti-diabetic, antibacterial, antifungal, antioxidant, anti-hemolytic, anticancer, cardio-protective, and Hepato-protection. The aim of study is to Isolation, identification, and quantification of some compounds from aerial parts of Cassia glauca since no phytochemical investigation had previously been done in Iraq for this plant. The aerial parts were defatted in n. hexane for 48 hours. The defatted materials were extracted in 85% ethanol using the hot method (soxhlet), then the extract was fractionated using different solvents (chloroform, ethyl acetate, and n-butanol). High-performance liquid chromatography (HPLC), was used for identification and quantification by using authenticated standards, and preparative layer chromatography (PLC) was used for the isolation of the identified compounds. The isolated compounds were identified after isolation by liquid chromatography Mass Spectrometry LC-MS/MS-Q-TOF method. The different chromatographic and spectroscopic methods results indicate the presence of luteolin and chlorogenic acid in the ethyl acetate fraction and n-butanol fraction respectively and estimated the quantity as (130.77µg/1g) for luteolin, and (0.0006%) for chlorogenic acid from 50g of plant material.

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Section: Lc-ms/ms-q-tof Resultsmentioning

confidence: 99%

Section: Figure 5 Calibration Curve Of Proposed Luteolin By Hplcmentioning

confidence: 99%

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Isolation, Identification, and Quantification of Two Compounds from Cassia glauca Cultivated in Iraq

Kanaan M Abdlkareem,

Kadhim

2023

IJPS

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“…This assay is a very practical and useful method to test plant extract toxicity (Burci et al, 2019). Membranes of erythrocytes contain high level of polyunsaturated fatty acids, which explain their fragility towards oxidation (Phuse and Khan, 2018). Plant extract content in saponins may explain its toxicity (Zheng et al, 2019).…”

Section: Discussionmentioning

confidence: 99%

“…Membranes of erythrocytes are susceptible to oxidize. This is mainly due by their richness in polyunsaturated lipids (Phuse and Khan, 2018). Plant extracts can have either anti-hemolytic effect in protecting erythrocytes or hemolytic induction anemia.…”

Section: Introductionmentioning

confidence: 99%

Antioxidant Activity and Hemolytic Effect of Hydro-methanolic Extract and its Phenolic Enriched Fractions From Leaves and Stems of Salvia officinalis L. from Algeria

Mahdi¹,

Azzi²,

Lahfa³

2021

jnpra

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This study aimed to assess an in vitro antioxidant and hemolytic activities of crude hydromethanolic extract, ethyl acetate and n-butanol fractions from leaves and stems of Salvia officinalis L. Extract and fractions prepared by maceration from S. officinalis were determined for their phytochemical composition and their contents in total phenolic and flavonoid. Antioxidant activity was evaluated by the methods of DPPH radical scavenging, ferric reducing antioxidant power and total antioxidant capacity. Hemolysis assay was carried out to evaluate the toxicity of the studied samples. Crude extract and fractions from S. officinalis contain different secondary metabolites and considerable contents of phenolic and flavonoids. They exhibit high antioxidant activity and low hemolytic effect. However, ethyl acetate fraction is characterized by the highest total phenolic (362.75 ± 0.07 µg GAE/mg DE) and flavonoid (263.27 ± 0.1 µg CE/mg DE) contents. Furthermore, this fraction shows the best antioxidant activity tested by DPPH, FRAP and TAC assays. The antiradical activity of ethyl acetate fraction against DPPH (IC50 = 208.51 ± 5.77 μg/mL) is close to that of ascorbic acid (IC50 = 206.43 ± 4.16 μg/mL). It exhibits a powerful activity to reduce iron (EC50 = 250 ± 0.001 μg/mL) and the best total antioxidant capacity (817.33 ± 0.06 μg/mL). It reveals the lowest hemolysis rate of 3.77 ± 0.02% with 200 mg/mL of fraction, after one hour of incubation. The obtained results suggest that crude extract, ethyl acetate and n-butanol fractions from Salvia officinalis L. are considered efficient natural antioxidants and safe for human erythrocytes. REFERENCES: Andrei, M., Cadmiel, M., Simone, C., Marcello, L., Marina, S., & Gianina, C. (2018).Determination of phenolic compounds in different species of salvia from Romania(Salvia transsylvanica, Salvia glutinosa, Salvia officinalis) and their biologicalactivities. 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Evaluation of in vitro α-amylase and α-glucosidase inhibitory potential and hemolytic effect of phenolic enriched fractions of the aerial part of Salvia officinalis L.

Mahdi

Azzi

Lahfa

2020

Diabetes & Metabolic Syndrome: Clinical Research & Revi

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Assessment of hemolytic effect of Cassia flower extracts on human RBCs

Cited by 3 publications

References 0 publications

Isolation, Identification, and Quantification of Two Compounds from Cassia glauca Cultivated in Iraq

Isolation, Identification, and Quantification of Two Compounds from Cassia glauca Cultivated in Iraq

Antioxidant Activity and Hemolytic Effect of Hydro-methanolic Extract and its Phenolic Enriched Fractions From Leaves and Stems of Salvia officinalis L. from Algeria

Evaluation of in vitro α-amylase and α-glucosidase inhibitory potential and hemolytic effect of phenolic enriched fractions of the aerial part of Salvia officinalis L.

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