Assessment of genetic variability within and among coffee progenies and cultivars using RAPD markers

Abstract

The RAPD technique associated with restriction digestion of genomic DNA was used to assess the genetic variability within and among nine populations of Coffea arabica, including six progenies belonging to the Sarchimor germplasm, the progeny PR 77054-40-10 (Catuaí Vermelho IAC 81 x Icatu), and two commercial cultivars (IAPAR 59 and Catuaí Vermelho IAC-81). These populations were evaluated using analysis of molecular variance (AMOVA), genetic similarity among progenies, and percentage of polymorphic loci. A total of 99 RAPD markers were evaluated of which 67 (67.67%) were polymorphic. AMOVA showed that 38.5% and 61.5% of the genetic variation was distributed among and within populations, respectively. The fixation index (F ST ) of the genotypes was 0.385. The mean genetic variability estimated within populations ranged from 15.58 (IAPAR 59) to 8.27 (Catuaí Vermelho IAC 81). A distinct level of genetic variability was revealed for each of the coffee progenies and varieties studied. The methodology used in this investigation was useful to determine the genetic variability within and among C. arabica L. populations providing significant information for coffee breeding.

Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

“…In wheat genotypes, Koebner (1995) observed that digestion of template DNA prior to PCR amplification allowed for more efficiency of primer annealing along shorter DNA fragment, where a simplified secondary structure was less likely to interfere with the process. Similar results were obtained by Sera et al (2003) in 14 accessions of arabica coffee and by Silveira et al (2003) in a study of genetic variability within and among Sarchimor progenies.…”

Genetic divergence in Conilon coffee revealed by RAPD markers

“…In the genus Coffea, several studies have shown the efficiency of RAPD markers for the genotype characterization, assessment and identification and for genetic mapping (Maluf et al 2005, Teixeira-Cabral et al 2004, Silveira et al 2003, Fontes et al 2000, Lashermes et al 1996, OrozcoCastillo et al 1994. A disadvantage of this marker type is its dominance, which impairs the distinction of polymorphism of heterozygous from homozygous dominant genotypes.…”

Genetic diversity among commercial coffee varieties, advanced selections and museum collections in Kenya using molecular markers

“…Different lines of HDT have been used worldwide to breed coffee varieties that are resistant to different pathogens. As would be expected, different accessions of HDT derivatives have different levels of introgressed C. canephora genome Silveira et al, 2003). This could explain the close relationship observed between HDT, Ruiru 11 and Catimor Line 90 to Robusta.…”