Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

Diniz, L. E. C.; Ruas, Claudete de Fátima; Carvalho, Valdemar de Paula; Torres, Fabrício Medeiros; Ruas, Eduardo Augusto; Santos, Mariana; Sera, Tumoru; Ruas, Paulo Maurício

doi:10.1590/s1516-89132005000500002

Cited by 23 publications

(20 citation statements)

References 16 publications

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“…RAPD have been used for different number of species, independent of the strategy of reproduction, with success as in Coffea arabica autogamous (Diniz et al 2005); C. canephora allogamous (Ferrão et al 2009), Aspidosperma allogamous (Torezan et al 2005), Zea mays self-fertilized (Souza et al 2008), Catharantus roseus (Shaw et al 2009), Cassava allogamous (Ferreira et al 2008). Chiari et al (2007) highlighted the importance of knowing the genetic variability in the Brachiaria germplasm bank accesses for successful plant breeding plans, planning of divergent crosses and guidance of new collections or germplasm exchange.…”

Section: Ac Ambiel Et Almentioning

confidence: 99%

Brachiaria germplasm dissimilarity as shown by RAPD markers

Ambiel¹,

Neto²,

Guaberto³

et al. 2010

CBAB

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Section: Ac Ambiel Et Almentioning

confidence: 99%

Brachiaria germplasm dissimilarity as shown by RAPD markers

Ambiel¹,

Neto²,

Guaberto³

et al. 2010

CBAB

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“…The first step in the search for heterosis in crop improvement is a full characterisation of available genetic diversity, which forms the basis for the analysis of inbred lines combining ability (Verbitskaya et al, 1999;Diniz et al, 2005). Genetic diversity is the most important factor limiting the average number of alleles identified per Simple Sequence Repeats (SSR) locus during screening programmes (Legesse, 2007).…”

Section: Introductionmentioning

confidence: 99%

Microsatellite analysis of the correlation between molecular and morphological traits in assorted maize inbred lines

Karanja¹,

Amugune²,

Ininda³

et al. 2010

African Crop Science Journal

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The success in identifying heterosis in hybrid maize (Zea mays L.) breeding depends on the availability of reliable genetic diversity among maize inbred lines. Conventional methods of breeding have been boosted by the availability and efficiency of molecular markers. Coupling simple sequence repeat (SSR) markers with morphological markers provides thorough starting information for new inbred lines, especially from different genetic backgrounds. Furthermore, recent evidences that the environment can influence the epigenetic structure of the genome have necessitated morphological screening of crops during breeding programmes. This study used 28 agronomic traits and 14 SSR markers which are distributed uniformly in ten (1-10) inbred lines, namely EM11-133, EM12-210, OSU23i, CML395, CML202, CML442, CML444, CML208, CML312 and CML204 from Kenya, International Centre for the Improvement of Maize and Wheat (CIMMYT), and another (OSU 23i) from USA. The aim was to investigate their morphological and genetic diversity, categorise the inbred lines into useful groups based on the molecular profiles and morphological traits, and lastly determine the level of phenotype-genotype correlation. The dissimilarity calculated using SSR markers had a mean morphological dissimilarity of 0.895403, an r value of -0.1421 and a p -0.9840. The dissimilarity between the molecular and morphological traits was 0.860465. Comparison between the molecular and morphological data had a dissimilarity matrix with an r -0.2323 and a p value of 0.0120. This was probably due to intrinsic synteny in maize genome. The dendrograms generated with hierarchical Unweighted Pair Group Method with Arithmetic mean (UPGMA) cluster analysis of the Jaccard's similarity coefficient matrices revealed four major clusters. The Co-ancestry distance showed six tied groups with the Kenya cluster showing some differentiation with Exact Tests for population differentiation with a p = 0.0513. The American inbred line (OSU 23i) segregated alone, while the Kenya lines (EM11-133 and EM12-210) had close homology with the CIMMYT inbred lines (CMLs). A total of 2.0 alleles were detected among the inbred lines using bulk DNA samples and 14 SSR loci. Clustering analysis based on the genetic similarity coefficients separated the inbred lines into 4 groups with the American inbred line seeming to be genotypically more diverse from the others.Key Words: Genetic diversity, molecular markers, SSR, Zea mays RÉSUMÉLe succès dans l'identification des hétérosis de maïs hybrides (Zea mays L.) dépend de la disponibilité d'une diversité génétique fiable dans les lignées endogames du maïs. Les méthodes conventionnelles de l'hybridation avaient été améliorées par la disponibilité et l'efficacité des marqueurs moléculaires. Le couplage des marqueurs simples de sequence répétée (SSR) avec les marqueurs morphologiques fournit des informations fondamentales précises pour les nouvelles lignées endogames, principalement de différente constitution génétique. En outre, les recentes évidences selon...

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“…The RAPD (random amplified polymorphic DNA) and AFLP (amplified fragment length polymorphism) are genetic fingerprinting techniques suitable for the genetic evaluation of flue-cured tobacco. The techniques have been successfully used to genetically analyze many different plant species (Crochemore et al, 2003;Diniz et al, 2005;Ni et al, 2006 andYang et al, 2006). In this work, the RAPD and AFLP procedures were used to assess the amount of polymorphisms detected among the flue-cured tobacco cultivars and to estimate the relationships.…”

Section: Introductionmentioning

confidence: 99%

Genetic Diversity among Flue-cured Tobacco Cultivars Based on RAPD and AFLP Markers

Zhang¹,

Liu²,

et al. 2008

Braz. arch. biol. technol.

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The aim of this work was to study the genetic diversity among flue-cured tobacco cultivars. RAPD and AFLP analyses were used to assess the genetic similarity among selected accessions of flue-cured tobacco. Seventy eight RAPD and 154 AFLP polymorphic bands were obtained and used to assess the genetic diversity among 28 tobacco accessions. The cultivar relationships were estimated through the cluster analysis (UPGMA) based on RAPD dataand AFLP data. The accessions were grouped into three major clusters and these shared common ancestry clustered together.

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Genetic diversity among forty coffee varieties assessed by RAPD markers associated with restriction digestion

Abstract: The genetic variability of 40 accessions of C. arabica was evaluated using a combination of the random amplified polymorphic DNA (RAPD

Cited by 23 publications

References 16 publications

Brachiaria germplasm dissimilarity as shown by RAPD markers

Brachiaria germplasm dissimilarity as shown by RAPD markers

Microsatellite analysis of the correlation between molecular and morphological traits in assorted maize inbred lines

Genetic Diversity among Flue-cured Tobacco Cultivars Based on RAPD and AFLP Markers

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