Genetic divergence in Conilon coffee revealed by RAPD markers

Abstract

-This study aimed to evaluate the genetic variability of 49 Coffea canephora clones of the breeding program of the Capixaba Institute of Research, Technical Assistance and Rural Extension (Incaper) based on RAPD markers. Thirty-one primers were used with polymorphism patterns that generated 333 markers, of which 231 (69.4%) were polymorphic. The group of genotypes based on the UPGMA algorithm and Tocher optimization methods detected high divergence in the genotypes. It was found that the component clones of each clone variety recommended by Incaper are distributed in various genetically dissimilar groups, in spite of common phenotypic traits. The relatively wide genetic diversity observed here demonstrates the importance of hybridizations between these germplasms. The RAPD markers efficiently estimated the genetic divergence in the 49 C. canephora genotypes studied.

Brachiaria germplasm dissimilarity as shown by RAPD markers

“…RAPD have been used for different number of species, independent of the strategy of reproduction, with success as in Coffea arabica autogamous (Diniz et al 2005); C. canephora allogamous (Ferrão et al 2009), Aspidosperma allogamous (Torezan et al 2005), Zea mays self-fertilized (Souza et al 2008), Catharantus roseus (Shaw et al 2009), Cassava allogamous (Ferreira et al 2008). Chiari et al (2007) highlighted the importance of knowing the genetic variability in the Brachiaria germplasm bank accesses for successful plant breeding plans, planning of divergent crosses and guidance of new collections or germplasm exchange.…”

Genetic diversity in yellow passion fruit (Passiflora edulis Sims) based on RAPD

“…In these studies a variable number of polymorphic bands was observed, for example, 30 polymorphic bands by Leal et al (2008) and 231 polymorphic bands obtained and evaluated by Ferrão et al (2009). Variation in the number of RAPD markers used in diversity studies is also demonstrated and discussed by Dias et al (2004).…”

“…The application of RAPD markers is not restricted to the characterization of the genus Passiflora and the use efficiency of this technique in different genetic approaches is demonstrated by recent results in solving problems in different plant species, generating useful information for conservation and genetic improvement of plants (Juchum et al 2007, Leal et al 2008, Ferrão et al 2009). In these studies a variable number of polymorphic bands was observed, for example, 30 polymorphic bands by Leal et al (2008) and 231 polymorphic bands obtained and evaluated by Ferrão et al (2009).…”

Genetic gain in the productivity of processed coffee from the selection of clones of 'Conilon' coffee

“…A alta diversidade entre plantas de uma mesma lavoura é característica marcante dessa espécie que apresenta alta variabilidade genética natural e polinização cruzada entre gerações (FERRÃO et al, 2009;FONSECA et al, 2006). Essa heterogeneidade, causada pela segregação genética, dificulta os tratos culturais e diminui a produtividade média da lavoura, uma vez que resulta em uma distribuição normal de plantas com maior e menor produtividade de grãos.…”