Assessment of genetic diversity in cultivated radishes ( Raphanus sativus ) by agronomic traits and SSR markers

Lee, O New; Park, Han Yong

doi:10.1016/j.scienta.2017.05.025

Cited by 28 publications

(24 citation statements)

References 31 publications

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“…Cruciferous vegetables represent a good source of active compounds as a base for further applications. From cruciferous vegetables, Raphanus sativus L. (radish) represents a very important vegetal crop all over the world, widely consumed as a condiment or a vegetable in human diets [25]. The aerial part of radish (leaves) has long been grown as a feed crop, but it also has various medicinal actions, harvesting their antimicrobial and antiviral [26], or antioxidant potential [27].…”

Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

Ungureanu

Fierăscu

et al. 2021

Materials

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The aim of the current paper is the development of phytosynthesized silver nanoparticles mediated by Raphanus sativus L. extracts obtained through two extraction methods (temperature and microwave) and to test their potential application for controlling apple crops pathogens. The phytosynthesized materials were characterized by X-ray diffraction, scanning electron microscopy, and transmission electron microscopy. All the materials were evaluated in terms of antioxidant and in vitro antimicrobial activity (against bacteria, molds, and yeast: Escherichia coli ATCC 8738, Staphylococcus aureus ATTC 25923, Pseudomonas aeruginosa ATCC 9027, Salmonella typhimurium ATCC 14028, Candida albicans ATCC 10231, Venturia inaequalis, Podosphaera leucotricha, Fusarium oxysporum ATCC 48112, Penicillium hirsutum ATCC 52323, and Aspergillus niger ATCC 15475). Considering the results obtained in the in vitro assays, formulations based on nanoparticles phytosynthesized using Raphanus sativus L. waste extracts (RS1N) were evaluated as potential antifungal agents for horticultural crops protection, against Venturia inaequalis and Podosphaera leucotricha through in vivo assays. For the DPPH assay, the inhibition (%) varied between 37.06% (for RS1N at 0.8 mg/mL concentration) and 83.72% (for RS1N at 7.2 mg/mL concentration) compared to 19.97% (for RS2N at 0.8 mg/mL) and only 28.91% (for RS2N at 7.2 mg/mL). Similar results were obtained for RS3N (85.42% inhibition at 7.2 mg/mL) compared with RS4N (21.76% inhibition at 7.2 mg/mL). Regarding the ABTS assay, the highest scavenger activity values were recorded for samples RS1N (91.43% at 1.6 mg/mL) and RS3N (96.62% at 1.6 mg/mL).

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Section: Introductionmentioning

confidence: 99%

In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

Ungureanu

Fierăscu

et al. 2021

Materials

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“…112 alleles were detected in the 129 wax gourd accessions using the 32 markers and the number of different alleles (Na) for each marker ranged from 2 to 9, and their PIC values ranged from 0.118 to 0.832 with an average of 0.370. The average of alleles and PIC values were lower than studies in bottle gourd [48], radish [46] and watermelon [47]. The lower levels of polymorphism may be caused by the narrow genetic background of 129 wax gourd accessions, which were all collected from China.…”

Section: Discussionmentioning

confidence: 57%

“…SSR markers have been used in the genetic analysis of many horticultural crops [12,21,[46][47][48]. Though there are a few SSR markers of wax gourd developed from the transcriptome [3], the genetic diversity of wax gourds has been rarely analyzed in previous studies.…”

Section: Discussionmentioning

confidence: 99%

Genome Wide SSR Development and Their Application in Genetic Diversity Analysis in Wax Gourd

Wang

Jiang

et al. 2021

Preprint

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Background: Wax gourd (Benincasa hispida Cong., 2n=2x=24) is one of the most important winter vegetables of the Cucurbitaceae family. There are only limited markers available for this crop and the draft genome of wax gourd provides a powerful tool for SSR marker development.Results: In this study, we developed genome-wide SSR markers from wax gourd genome and characterized their distribution and frequency of different motifs and repeats. A total of 52,431 microsatellites from wax gourd genome were identified, of which 39,319 SSR markers were developed. 1,152 non-wax gourd SSR markers were selected from cucumber, melon, watermelon and pumpkin to test their transferability in wax gourd. 580 SSR markers could be transferable in wax gourd, and 42 of them were detected with polymorphic in 11 tested accessions of wax gourd. In addition, 11 good polymorphic transferrable SSR markers and 21 SSR markers of wax gourd were selected to investigate the genetic diversity and population structure of 129 wax gourd accessions. 112 alleles were detected by these 32 SSR markers. The result of population structure showed that the 129 wax gourd accessions were divided into two main populations, and the genetic diversity analysis separated them into two clusters. Conclusions: The large number of wax gourd SSR markers developed in this study provides a valuable resource for genetic linkage map construction, molecular mapping, and marker-assisted selection (MAS) in wax gourd.

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“…Also, in the Zambezia province, 12 additional accessions were collected in three communities of the AEZ R7, a medium altitude zone spanning across Zambezia and Tete provinces in Central Mozambique and Nampula, Niassa and Cabo Delgado provinces in Northern Mozambique. In central Mozambique drylands, encompassing the AEZ R6, in the Manica province, 26 accessions were collected in the Tambara central Business District (23) and Sede Nova (3). In the Sofala province, three accessions were collected in Maringué, within AEZ R5, which covers mainly inland and coastal areas of the lower Sofala.…”

Section: Plant Materials and Morphological Characterizationmentioning

confidence: 99%

“…Morphological traits have for years formed the backbone of breeders' attempts to capture the phenotypic differences among genotypes not only in cowpea [22], but also in several other crops worldwide [23][24][25]. These traits are a straightforward tool to estimate diversity and select parental lines for crossing [23], particularly in financially constrained agricultural research and development contexts such as the ones occurring in Africa. Thus, despite the important value of cowpea in Mozambique, to our knowledge, our study provides the most comprehensive investigation of the morphological diversity existing in the landraces of that country.…”

Section: Importance Of Morphological Traits In Cowpea Landracesmentioning

confidence: 99%

Diversity of Cowpea [Vigna unguiculata (L.) Walp] Landraces in Mozambique: New Opportunities for Crop Improvement and Future Breeding Programs

et al. 2021

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Cowpea (Vigna unguiculata) is a neglected crop native to Africa, with an outstanding potential to contribute to the major challenges in food and nutrition security, as well as in agricultural sustainability. Two major issues regarding cowpea research have been highlighted in recent years—the establishment of core collections and the characterization of landraces—as crucial to the implementation of environmentally resilient and nutrition-sensitive production systems. In this work, we have collected, mapped, and characterized the morphological attributes of 61 cowpea genotypes, from 10 landraces spanning across six agro-ecological zones and three provinces in Mozambique. Our results reveal that local landraces retain a high level of morphological diversity without a specific geographical pattern, suggesting the existence of gene flow. Nevertheless, accessions from one landrace, i.e., Maringué, seem to be the most promising in terms of yield and nutrition-related parameters, and could therefore be integrated into the ongoing conservation and breeding efforts in the region towards the production of elite varieties of cowpea.

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Assessment of genetic diversity in cultivated radishes ( Raphanus sativus ) by agronomic traits and SSR markers

Cited by 28 publications

References 31 publications

In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

In Vitro and In Vivo Evaluation of Silver Nanoparticles Phytosynthesized Using Raphanus sativus L. Waste Extracts

Genome Wide SSR Development and Their Application in Genetic Diversity Analysis in Wax Gourd

Diversity of Cowpea [Vigna unguiculata (L.) Walp] Landraces in Mozambique: New Opportunities for Crop Improvement and Future Breeding Programs

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