Drought threatens the world's food production, particularly in Sub Saharan Africa low external input and rain fed agricultural systems, where cowpea (Vigna unguiculata (L.) Walp.) is an important food crop. In the context of growing concerns regarding climate changes implications on water availability, this study aimed at 1) to evaluate the drought responses in cowpea landraces with contrasting drought tolerance levels (A55high sensitivity; A80 -mild sensitivity; A116 -tolerant), 2) using an integrated physiological (leaf gas exchanges; chlorophyll a fluorescence) and biochemical (photoprotective pigments; RuBisCO activity; primary metabolite profiling) analysis to identify drought tolerance probes, in plants submitted to three water availability levels (well-watered, WW; mild drought, MD; severe drought, SD). A116 plants maintained a better water status under drought, what could justify the higher P n and P nmax values in MD, as well as higher photochemical use of energy (reflected in the photochemical quenching (q L ) and in the quantum yield of non-cyclic electron transport (Y (II) )), and the lower need of photoprotective thermal dissipation mechanisms (given by the non-photochemical quenching (q N ), and the quantum yield of regulated energy dissipation at photosystem PSII (Y (NPQ) )), in MD and SD plants. Greater declines of net (P n ) and potential (P nmax ) photosynthesis were observed in A55 plants, which frequently showed significant impacts already under MD conditions in most parameters, whereas A80 usually displayed and intermediate behaviour. Still, even A55 showed some acclimation response, regarding photoprotective mechanisms associated with high contents of zeaxanthin, lutein, and carotenes, and high Y (NPQ) , and q N values, supporting the absence of an increase in the non-regulated energy dissipation at PSII (Y (NO) did not increased) even in SD plants. Additionally, A55 was not significantly affected in RuBisCO activity, which showed to be quite resilient in cowpea. A primary metabolite profiling, complemented with a partial least square discrimination analysis (PLS-DA), allowed a better separation of A116 and A55 plants according to their degree of drought tolerance. In response to drought, A116 showed the greatest accumulation of most responsive metabolites, 14 in total, with sucrose, fucose, urea, alanine and putrescine being exclusively increased in this genotype, suggesting that they can be candidates as drought tolerance proxies. Other compounds, as proline, valine, isoleucine (among amino acids), and rhamnose and raffinose (among sugars) showed close increase patterns
Cowpea is a multiple-purpose drought-tolerant leguminous pulse crop grown in several dry tropical areas. Its domestication center is thought to be East or West Africa, where a high level of genetic diversity is apparently still found. However, detailed genetic information is lacking in many African countries, limiting the success of breeding programs. In this work, we assessed the genetic variation and gene flow in 59 Vigna unguiculata (cowpea) accessions from 10 landraces spanning across six agro-ecological zones of Mozambique, based on nuclear microsatellite markers. The results revealed the existence of high genetic diversity between the landraces, even in comparison to other world regions. Four genetic groups were found, with no specific geographic pattern, suggesting the presence of gene flow between landraces. In comparison, the two commercial varieties had lower values of genetic diversity, although still close to the ones found in local landraces. The high genetic diversity found in Mozambique sustains the importance of local genetic resources and farm protection to enhance genetic diversity in modern varieties of cowpea worldwide.
Cowpea (Vigna unguiculata) is a neglected crop native to Africa, with an outstanding potential to contribute to the major challenges in food and nutrition security, as well as in agricultural sustainability. Two major issues regarding cowpea research have been highlighted in recent years—the establishment of core collections and the characterization of landraces—as crucial to the implementation of environmentally resilient and nutrition-sensitive production systems. In this work, we have collected, mapped, and characterized the morphological attributes of 61 cowpea genotypes, from 10 landraces spanning across six agro-ecological zones and three provinces in Mozambique. Our results reveal that local landraces retain a high level of morphological diversity without a specific geographical pattern, suggesting the existence of gene flow. Nevertheless, accessions from one landrace, i.e., Maringué, seem to be the most promising in terms of yield and nutrition-related parameters, and could therefore be integrated into the ongoing conservation and breeding efforts in the region towards the production of elite varieties of cowpea.
Agriculture is threatened by ever increasing temperatures and this trend is predicted to continue for the near and distant future. The negative impact of rising temperatures on agri-food systems is also compounded by the erratic and highly variable rainfall in most parts of southern Africa. Minimum and maximum temperatures’ variability and trend analysis were undertaken using daily time series data derived from 23 meteorological stations spread across Malawi, Mozambique, South Africa and Zimbabwe. The modified Mann–Kendall and Theil–Sen slope models were used to assess temperature trends and their magnitudes. Temperature varied with location and minimum temperature was more variable than maximum temperature. Semi-arid regions had higher variation in minimum temperature compared to humid and coastal environments. The results showed an upward trend in minimum (0.01–0.83 °C over a 33–38 year period) and maximum (0.01–0.09 °C over a 38–57 year period) temperatures at 9 and15 locations, respectively. A downward trend in minimum temperature (0.03–0.20 °C over 38–41 years) occurred in South Africa at two locations and Dedza (Malawi), while a non-significant decline in maximum temperature (0.01 °C over 54 years) occurred at one location in coastal dry sub-humid Mozambique. The results confirm the increase in temperature over 33–79 years, and highlight the importance of including temperature when designing climate change adaption and mitigation strategies in southern Africa and similar environments.
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