Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria

Shuaibu, Iliyasu Yunusa; Hadiza, J. A.; Yusha’u, M; Kabiru, M. Y.; Ahmad, Mani Malam; Garba, Lawal; Adamu, M.T.; Khairiyya, M

doi:10.17485/ijst/2016/v9i48/109624

Cited by 3 publications

(2 citation statements)

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“…It was concluded that there exists a rather high contamination level in home-made tiger nut beverages indicating the need to apply correct and strict hazard analysis and critical control point (HACCP) systems during manufacturing and storage. In Nigeria, many workers [46][47][48][49][50][51][52][53][54][55] have carried out a microbiological analysis on TNM and Aspergillus, Candida, and Saccharomyces were found to be the main fungal isolates, whereas Bacillus, Escherichia and Salmonella were the main bacterial isolates (Table 1). Salmonella can transmit typhoid fever and could occur from the water used for processing.…”

Section: Tiger Nut Milkmentioning

confidence: 99%

Traditional and Artisanal Beverages in Nigeria: Microbial Diversity and Safety Issues

et al. 2020

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A review of up to 90 articles on the microorganisms associated with important artisanal or traditional beverages in Nigeria was carried out. This resulted in an overview of the prevalent microorganisms associated with soymilk, nono (fermented cow milk), tiger nut milk, yoghurt, kunu, zobo, palm wine and the local beers pito and brukutu. The bacteria genera, namely Bacillus, Escherichia, Lactobacillus, Staphylococcus, and Streptococcus, were detected in all nine beverages. On the contrary, this survey resulted in finding that the genera Saccharomyces, Aspergillus, Candida, and Penicillium were the eukaryotic microorganisms isolated in all beverages. The occurrence of fungal isolates, which can be responsible for producing mycotoxins, is a concern and shows the need for post-production tests. Overall, there is a low prevalence of bacteria associated with hygiene, especially the Escherichia genus in alcoholic beverages such as palm wine, pito and burukutu, which may be due both to a low acidity and high ethanol content. However, the prevalence of hygiene indicator genera was higher in nonalcoholic drinks, probably because of incorrect practices during processing. The magnitude of the production and sales of unregulated local beverages in Nigeria has reached the stage where significant regulation and food safety standards are required to safeguard public health. An opportunity exists to monitor and characterize the microbial flora of the artisanal beverages using molecular methods at all stages of production and storage.

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Section: Tiger Nut Milkmentioning

confidence: 99%

Traditional and Artisanal Beverages in Nigeria: Microbial Diversity and Safety Issues

et al. 2020

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“…Identified risk factors for developing ESBL producers include prolonged hospitalization and indiscriminate use/abuse of antibiotics [25] [29]. Even though several studies have been carried out on the prevalence of ESBL, few have been associated with urinary tract infections, especially in north-eastern Nigeria where the prevalence of urinary tract infection is reported to be as high as 33.34% in Gombe [30]. It is in view of this, that this study was designed to evaluate the prevalence and molecular nature of ESBL in isolates obtained from patients with urinary tract infections in Gombe, Nigeria.…”

Section: Introductionmentioning

confidence: 99%

Occurrence of Extended Spectrum Beta Lactamase Encoding Genes among Urinary Pathogenic <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates Obtained from a Tertiary Hospital in Gombe Nigeria

Yarima¹,

Haroun²,

Bulus³

et al. 2020

JBM

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This study was conducted to assess the occurrence and nature of extended-spectrum beta lactamase (ESBL) producing Escherichia coli and Klebsiella pneumoniae isolates from patients who presented with urinary tract infection at Federal Teaching Hospital Gombe. Isolates collected were recovered on MacConkey agar at 35˚C and were identified as members of Enterobacteriaceae, and further screened for antimicrobial susceptibility and resistance by disc diffusion method. Isolates resistant to oxyimino-cephalosporins were confirmed as ESBL producers using Double Disks Synergy Test (DDST). The study shows 66% resistance to ceftriaxone (30 µg) in K. pneumoniae, which was the highest value recorded and a 51% resistance to cefpodoxime (10 µg) in E. coli. The sensitivity of E. coli and K. pneumoniae isolates to cefpodoxime (10 µg) were 49% and 33.9% respectively. ESBLs were detected among 40% (40/100) of E. coli and 54.13% (59/109) of K. pneumoniae isolates. Molecular characterization of ESBL encoding genes among E. coli isolates using multiplex-PCR showed 10% prevalence of SHV gene and 5% prevalence for CTX-M gene while TEM gene was not detected. In K. pneumoniae isolates, 5% prevalence was recorded for each of the three genes screened. The study revealed a co-occurrence of SHV and CTX-M in 75% of the E. coli and 70% of the K. pneumoniae isolates; the occurrence of all the three genes was seen in 10% and 5% of K.

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Potential in vitro inhibitory effects of Moringa oleifera leaf extracts on extended-spectrum β-lactamase-producing bacteria

Garba¹,

Muhammad²,

Adamu³

et al. 2021

J. Fundam and Appl Sci.

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Extended-spectrum β-lactamase (ESβL) enzymes are produced by certain bacteria as a mechanism of resistance to β-lactam and extended-spectrumβ-lactam antibiotics. Previous investigations have confirmed that M. oleifera contains several bioactive compounds. This study aimed at evaluating the in vitro antibacterial effects of ethanolic and methanolic leaf extracts of M. oleifera on extended-spectrum β-lactamase producing bacteria. The leaf extracts of the plant were prepared, screened for bioactive compounds and evaluated for in vitro inhibitory effects against the ESβL-producing bacteria using agar well diffusion method. Different phytochemicals were detected from the extracts. Both methanolic and ethanolic leaf extracts showed a high inhibitory activity, which increased with an increase in concentration, from lowest to highest based on the zones of growth inhibition produced. Amongst the test organisms, Klebsiella pneumoniae was the most sensitive isolate to methanolic extract at 200, 100, 50 and 25mg/mL followed by Escherichia coli, and then Proteus mirabilis. However, in terms of the ethanolic extract, using the same concentrations as those of methanol extracts, E. coli was found to be the most sensitive isolate followed by K. pneumoniae and then P. mirabilis.

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Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria

Cited by 3 publications

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Traditional and Artisanal Beverages in Nigeria: Microbial Diversity and Safety Issues

Traditional and Artisanal Beverages in Nigeria: Microbial Diversity and Safety Issues

Occurrence of Extended Spectrum Beta Lactamase Encoding Genes among Urinary Pathogenic <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates Obtained from a Tertiary Hospital in Gombe Nigeria

Potential in vitro inhibitory effects of Moringa oleifera leaf extracts on extended-spectrum β-lactamase-producing bacteria

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Assessment of Foods and Drinks for the Presence of Extended Spectrum Beta Lactamase (ESBL) Producing Bacteria in Gombe Metropolis, Nigeria

Cited by 3 publications

References 0 publications

Traditional and Artisanal Beverages in Nigeria: Microbial Diversity and Safety Issues

Traditional and Artisanal Beverages in Nigeria: Microbial Diversity and Safety Issues

Occurrence of Extended Spectrum Beta Lactamase Encoding Genes among Urinary Pathogenic &lt;i&gt;Escherichia coli&lt;/i&gt; and &lt;i&gt;Klebsiella pneumoniae&lt;/i&gt; Isolates Obtained from a Tertiary Hospital in Gombe Nigeria

Potential in vitro inhibitory effects of Moringa oleifera leaf extracts on extended-spectrum β-lactamase-producing bacteria

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Occurrence of Extended Spectrum Beta Lactamase Encoding Genes among Urinary Pathogenic <i>Escherichia coli</i> and <i>Klebsiella pneumoniae</i> Isolates Obtained from a Tertiary Hospital in Gombe Nigeria